O65896 · CDA1_ARATH

Function

function

This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Functions as a conventional cytidine deaminase. Has no affinity for RNA and is not involved in RNA-editing by C-to-U deamination.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Activity regulation

Inhibited by uridine, CMP and dCMP.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
150 μMcytidine7.537
250 μMcytidine7.525
226.1 μMcytidine8.025
75 μM2'-deoxycytidine7.537
120 μM2'-deoxycytidine7.525
49.3 μM2'-deoxycytidine8.025
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
59.5 μmol/min/mg7.537toward cytidine
58 μmol/min/mg7.525toward cytidine
39.7 μmol/min/mg8.025toward cytidine
49 μmol/min/mg7.537toward 2'-deoxycytidine
38 μmol/min/mg7.525toward 2'-deoxycytidine
24.4 μmol/min/mg8.025toward 2'-deoxycytidine, Ref.3

pH Dependence

Optimum pH is 7.5-8.0.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site64-66substrate
Binding site77Zn2+ (UniProtKB | ChEBI); catalytic
Active site79Proton donor
Binding site104Zn2+ (UniProtKB | ChEBI); catalytic
Binding site107Zn2+ (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentmitochondrion
Molecular Functioncytidine deaminase activity
Molecular Functionprotein homodimerization activity
Molecular Functionzinc ion binding
Biological Processcytidine catabolic process
Biological Processcytidine deamination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytidine deaminase 1
  • EC number
  • Short names
    At-CDA1

Gene names

    • Name
      CDA1
    • Synonyms
      CDD, DESZ
    • ORF names
      F3P11
    • Ordered locus names
      At2g19570

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Columbia
    • cv. Landsberg erecta
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    O65896
  • Secondary accessions
    • Q8LEF2
    • Q9XEX5

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004291431-301Cytidine deaminase 1

Proteomic databases

Expression

Tissue specificity

Expressed in roots, rosette leaves, stems and flowers.

Gene expression databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain23-156CMP/dCMP-type deaminase 1
Domain188-301CMP/dCMP-type deaminase 2

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    301
  • Mass (Da)
    32,582
  • Last updated
    1998-08-01 v1
  • Checksum
    91C17F09AA70CBB2
MDKPSFVIQSKEAESAAKQLGVSVIQLLPSLVKPAQSYARTPISKFNVAVVGLGSSGRIFLGVNVEFPNLPLHHSIHAEQFLVTNLTLNGERHLNFFAVSAAPCGHCRQFLQEIRDAPEIKILITDPNNSADSDSAADSDGFLRLGSFLPHRFGPDDLLGKDHPLLLESHDNHLKISDLDSICNGNTDSSADLKQTALAAANRSYAPYSLCPSGVSLVDCDGKVYRGWYMESAAYNPSMGPVQAALVDYVANGGGGGYERIVGAVLVEKEDAVVRQEHTARLLLETISPKCEFKVFHCYEA

Sequence caution

The sequence AAD30449.1 differs from that shown. Reason: Miscellaneous discrepancy Sequencing errors.

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict270in Ref. 8; AAM62679
Sequence conflict293in Ref. 8; AAM62679

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AJ005261
EMBL· GenBank· DDBJ
CAA06460.1
EMBL· GenBank· DDBJ
mRNA
AJ005687
EMBL· GenBank· DDBJ
CAA06671.1
EMBL· GenBank· DDBJ
Genomic DNA
AF134487
EMBL· GenBank· DDBJ
AAF03358.1
EMBL· GenBank· DDBJ
mRNA
AF121878
EMBL· GenBank· DDBJ
AAD30449.1
EMBL· GenBank· DDBJ
mRNA Sequence problems.
AC005917
EMBL· GenBank· DDBJ
AAD10156.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002685
EMBL· GenBank· DDBJ
AEC06896.1
EMBL· GenBank· DDBJ
Genomic DNA
AY128366
EMBL· GenBank· DDBJ
AAM91569.1
EMBL· GenBank· DDBJ
mRNA
BT008708
EMBL· GenBank· DDBJ
AAP42721.1
EMBL· GenBank· DDBJ
mRNA
AY085453
EMBL· GenBank· DDBJ
AAM62679.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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