O65896 · CDA1_ARATH
- ProteinCytidine deaminase 1
- GeneCDA1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids301 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Functions as a conventional cytidine deaminase. Has no affinity for RNA and is not involved in RNA-editing by C-to-U deamination.
Catalytic activity
- cytidine + H2O + H+ = uridine + NH4+This reaction proceeds in the forward direction.
Cofactor
Note: Binds 1 zinc ion per subunit.
Activity regulation
Inhibited by uridine, CMP and dCMP.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
150 μM | cytidine | 7.5 | 37 | |||
250 μM | cytidine | 7.5 | 25 | |||
226.1 μM | cytidine | 8.0 | 25 | |||
75 μM | 2'-deoxycytidine | 7.5 | 37 | |||
120 μM | 2'-deoxycytidine | 7.5 | 25 | |||
49.3 μM | 2'-deoxycytidine | 8.0 | 25 |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
59.5 μmol/min/mg | 7.5 | 37 | toward cytidine | ||
58 μmol/min/mg | 7.5 | 25 | toward cytidine | ||
39.7 μmol/min/mg | 8.0 | 25 | toward cytidine | ||
49 μmol/min/mg | 7.5 | 37 | toward 2'-deoxycytidine | ||
38 μmol/min/mg | 7.5 | 25 | toward 2'-deoxycytidine | ||
24.4 μmol/min/mg | 8.0 | 25 | toward 2'-deoxycytidine, Ref.3 |
pH Dependence
Optimum pH is 7.5-8.0.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 64-66 | substrate | |||
Binding site | 77 | Zn2+ (UniProtKB | ChEBI); catalytic | |||
Active site | 79 | Proton donor | |||
Binding site | 104 | Zn2+ (UniProtKB | ChEBI); catalytic | |||
Binding site | 107 | Zn2+ (UniProtKB | ChEBI); catalytic | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | mitochondrion | |
Molecular Function | cytidine deaminase activity | |
Molecular Function | protein homodimerization activity | |
Molecular Function | zinc ion binding | |
Biological Process | cytidine catabolic process | |
Biological Process | cytidine deamination |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytidine deaminase 1
- EC number
- Short namesAt-CDA1
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionO65896
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000429143 | 1-301 | Cytidine deaminase 1 | ||
Proteomic databases
Expression
Tissue specificity
Expressed in roots, rosette leaves, stems and flowers.
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 23-156 | CMP/dCMP-type deaminase 1 | |||
Domain | 188-301 | CMP/dCMP-type deaminase 2 | |||
Sequence similarities
Belongs to the cytidine and deoxycytidylate deaminase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length301
- Mass (Da)32,582
- Last updated1998-08-01 v1
- Checksum91C17F09AA70CBB2
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 270 | in Ref. 8; AAM62679 | |||
Sequence conflict | 293 | in Ref. 8; AAM62679 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ005261 EMBL· GenBank· DDBJ | CAA06460.1 EMBL· GenBank· DDBJ | mRNA | ||
AJ005687 EMBL· GenBank· DDBJ | CAA06671.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF134487 EMBL· GenBank· DDBJ | AAF03358.1 EMBL· GenBank· DDBJ | mRNA | ||
AF121878 EMBL· GenBank· DDBJ | AAD30449.1 EMBL· GenBank· DDBJ | mRNA | Sequence problems. | |
AC005917 EMBL· GenBank· DDBJ | AAD10156.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | AEC06896.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY128366 EMBL· GenBank· DDBJ | AAM91569.1 EMBL· GenBank· DDBJ | mRNA | ||
BT008708 EMBL· GenBank· DDBJ | AAP42721.1 EMBL· GenBank· DDBJ | mRNA | ||
AY085453 EMBL· GenBank· DDBJ | AAM62679.1 EMBL· GenBank· DDBJ | mRNA |