O65404 · ERG11_ARATH
- ProteinSqualene epoxidase 5
- GeneSQE5
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids516 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis.
Miscellaneous
SEQ4 or SEQ5 are unable to complement seq1 mutants.
Catalytic activity
- squalene + reduced [NADPH--hemoprotein reductase] + O2 = (S)-2,3-epoxysqualene + oxidized [NADPH--hemoprotein reductase] + H2O + H+
Cofactor
Pathway
Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 2/3.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 55-56 | FAD (UniProtKB | ChEBI) | |||
Binding site | 75-76 | FAD (UniProtKB | ChEBI) | |||
Binding site | 83 | FAD (UniProtKB | ChEBI) | |||
Binding site | 88 | FAD (UniProtKB | ChEBI) | |||
Binding site | 156 | FAD (UniProtKB | ChEBI) | |||
Binding site | 172 | FAD (UniProtKB | ChEBI) | |||
Binding site | 335 | FAD (UniProtKB | ChEBI) | |||
Binding site | 348 | FAD (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | squalene monooxygenase activity | |
Biological Process | sterol biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSqualene epoxidase 5
- EC number
- Short namesAtSQE5
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionO65404
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 3-23 | Helical | |||
Transmembrane | 45-65 | Helical | |||
Transmembrane | 446-466 | Helical | |||
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000209841 | 1-516 | Squalene epoxidase 5 | ||
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in seedlings, leaves, stems and inflorescences. Detected in siliques.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.
O65404-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length516
- Mass (Da)56,606
- Last updated2000-12-01 v2
- MD5 Checksum14975343D6E0261FC09A8F41E4F7EA37
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F4KFQ9 | F4KFQ9_ARATH | SQP1 | 490 |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB016883 EMBL· GenBank· DDBJ | BAB08406.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED93262.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AJ005930 EMBL· GenBank· DDBJ | CAA06772.1 EMBL· GenBank· DDBJ | mRNA |