O65351 · SBT17_ARATH

Function

function

Serine protease. Has a substrate preference for the hydrophobic residues Phe and Ala and the basic residue Asp in the P1 position, and for Asp, Leu or Ala in the P1' position (PubMed:12413398).
Essential for mucilage release from seed coats. Triggers the accumulation and/or activation of cell wall modifying enzymes necessary either for the loosening of the outer primary cell wall, or to facilitate swelling of the mucilage (PubMed:18266922).

Activity regulation

Activated by calcium. Inhibited by the serine protease inhibitors 4-(2-aminoethyl)benzenesulphonyl fluoride (AEBSF), PMSF, di-isopropyl phosphofluoridate (DFP) and soybean trypsin inhibitor (SBTI). Not inhibited by benzamidine or iodoacetamide. Leupeptin and pepstatin A have a minor inhibitory action.

pH Dependence

Optimum pH is 5.0.

Temperature Dependence

Optimum temperature is 80 degrees Celsius. Thermostable.

Features

Showing features for active site.

1757100200300400500600700
TypeIDPosition(s)Description
Active site139Charge relay system
Active site212Charge relay system
Active site542Charge relay system

GO annotations

AspectTerm
Cellular Componentapoplast
Cellular Componentextracellular region
Cellular Componentsecretory vesicle
Molecular Functionserine-type endopeptidase activity
Biological Processmucilage extrusion from seed coat
Biological Processmucilage metabolic process involved in seed coat development
Biological Processproteolysis
Biological Processseed coat development

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Subtilisin-like protease SBT1.7
  • EC number
  • Alternative names
    • Cucumisin-like serine protease
    • Subtilase subfamily 1 member 7
      (AtSBT1.7
      )
    • Subtilisin-like serine protease 1
      (At-SLP1
      )

Gene names

    • Name
      SBT1.7
    • Synonyms
      ARA12, ASP48
      , SLP1
    • ORF names
      K8K14.8
    • Ordered locus names
      At5g67360

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Columbia
    • cv. Landsberg erecta
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    O65351
  • Secondary accessions
    • P80854
    • Q39007
    • Q8RWQ7

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

No visible phenotype under normal growth conditions, but mutant seeds are defective in mucilage extrusion.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 47 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for signal, propeptide, chain, glycosylation.

TypeIDPosition(s)Description
Signal1-24
PropeptidePRO_000004284625-106
ChainPRO_0000042847107-757Subtilisin-like protease SBT1.7
Glycosylation170N-linked (GlcNAc...) asparagine
Glycosylation352N-linked (GlcNAc...) asparagine
Glycosylation376N-linked (GlcNAc...) asparagine
Glycosylation379N-linked (GlcNAc...) asparagine
Glycosylation631N-linked (GlcNAc...) asparagine
Glycosylation644N-linked (GlcNAc...) asparagine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in immature siliques and at lower levels in stems and flowers (PubMed:11055401, PubMed:12413398, PubMed:7647567).
Widely expressed at low levels (PubMed:18266922).

Induction

By methyl jasmonate.

Developmental stage

Highest levels of expression detected during silique development (PubMed:7647567).
Hihghly expressed in the seed coat during seed development (PubMed:18266922).

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, compositional bias, region.

TypeIDPosition(s)Description
Domain31-106Inhibitor I9
Domain102-610Peptidase S8
Compositional bias196-214Basic and acidic residues
Region196-219Disordered

Sequence similarities

Belongs to the peptidase S8 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    757
  • Mass (Da)
    79,415
  • Last updated
    1998-08-01 v1
  • Checksum
    E7F68FCAD16700AB
MSSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT

Features

Showing features for sequence conflict, compositional bias.

TypeIDPosition(s)Description
Sequence conflict41in Ref. 5; CAA59963
Compositional bias196-214Basic and acidic residues
Sequence conflict242-247in Ref. 5; CAA59963
Sequence conflict562in Ref. 4; AAM10321/AAN46863
Sequence conflict670in Ref. 5; CAA59963

Mass Spectrometry

Molecular mass is 76,102.8 Da. Determined by MALDI.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF065639
EMBL· GenBank· DDBJ
AAC18851.1
EMBL· GenBank· DDBJ
Genomic DNA
AB007645
EMBL· GenBank· DDBJ
BAB09021.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED98332.1
EMBL· GenBank· DDBJ
Genomic DNA
AF360285
EMBL· GenBank· DDBJ
AAK25995.1
EMBL· GenBank· DDBJ
mRNA
AY091773
EMBL· GenBank· DDBJ
AAM10321.1
EMBL· GenBank· DDBJ
mRNA
AY142612
EMBL· GenBank· DDBJ
AAN13181.1
EMBL· GenBank· DDBJ
mRNA
BT001082
EMBL· GenBank· DDBJ
AAN46863.1
EMBL· GenBank· DDBJ
mRNA
X85974
EMBL· GenBank· DDBJ
CAA59963.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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