O65202 · ACOX1_ARATH

Function

function

Catalyzes the desaturation of both long- and medium-chain acyl-CoAs to 2-trans-enoyl-CoAs. Most active with C14-CoA. Activity on long-chain mono-unsaturated substrates is 40% higher than with the corresponding saturated substrates. Seems to be an important factor in the general metabolism of root tips. May be involved in the biosynthesis of jasmonic acid.

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Note: Binds 1 FAD per subunit.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
5.3 μMC14-CoA

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site135FAD (UniProtKB | ChEBI)
Binding site137FAD (UniProtKB | ChEBI)
Binding site138FAD (UniProtKB | ChEBI)
Binding site144FAD (UniProtKB | ChEBI)
Binding site177FAD (UniProtKB | ChEBI)
Binding site310FAD (UniProtKB | ChEBI)
Binding site330FAD (UniProtKB | ChEBI)
Binding site333FAD (UniProtKB | ChEBI)
Binding site401FAD (UniProtKB | ChEBI)
Binding site422FAD (UniProtKB | ChEBI)
Active site424Proton acceptor
Binding site426FAD (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentperoxisome
Cellular Componentplasmodesma
Molecular Functionacyl-CoA oxidase activity
Molecular FunctionFAD binding
Biological Processfatty acid beta-oxidation
Biological Processjasmonic acid biosynthetic process
Biological Processlong-chain fatty acid metabolic process
Biological Processresponse to fungus
Biological Processresponse to wounding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Peroxisomal acyl-coenzyme A oxidase 1
  • EC number
  • Short names
    AOX 1
  • Alternative names
    • Long-chain acyl-CoA oxidase
      (AtCX1
      )

Gene names

    • Name
      ACX1
    • ORF names
      dl4405c
      , FCAALL.119
    • Ordered locus names
      At4g16760

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    O65202
  • Secondary accessions
    • O23518

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00002046891-664Peroxisomal acyl-coenzyme A oxidase 1
Disulfide bond467↔576

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed mainly in flowers and young seedlings. Lower expression in roots, leaves and bracts.

Induction

Induced by dehydration, abscisic acid (ABA) and jasmonic acid (JA), and locally and systemically by wounding.

Developmental stage

Induced by seed imbibition with a peak at day 2 and then declines to reach a basal level 4 days after sowing.

Gene expression databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif662-664Microbody targeting signal

Sequence similarities

Belongs to the acyl-CoA oxidase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete

This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.

O65202-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    664
  • Mass (Da)
    74,302
  • Last updated
    1998-08-01 v1
  • Checksum
    44AFD139D5434636
MEGIDHLADERNKAEFDVEDMKIVWAGSRHAFEVSDRIARLVASDPVFEKSNRARLSRKELFKSTLRKCAHAFKRIIELRLNEEEAGRLRHFIDQPAYVDLHWGMFVPAIKGQGTEEQQKKWLSLANKMQIIGCYAQTELGHGSNVQGLETTATFDPKTDEFVIHTPTQTASKWWPGGLGKVSTHAVVYARLITNGKDYGIHGFIVQLRSLEDHSPLPNITVGDIGTKMGNGAYNSMDNGFLMFDHVRIPRDQMLMRLSKVTREGEYVPSDVPKQLVYGTMVYVRQTIVADASNALSRAVCIATRYSAVRRQFGAHNGGIETQVIDYKTQQNRLFPLLASAYAFRFVGEWLKWLYTDVTERLAASDFATLPEAHACTAGLKSLTTTATADGIEECRKLCGGHGYLWCSGLPELFAVYVPACTYEGDNVVLQLQVARFLMKTVAQLGSGKVPVGTTAYMGRAAHLLQCRSGVQKAEDWLNPDVVLEAFEARALRMAVTCAKNLSKFENQEQGFQELLADLVEAAIAHCQLIVVSKFIAKLEQDIGGKGVKKQLNNLCYIYALYLLHKHLGDFLSTNCITPKQASLANDQLRSLYTQVRPNAVALVDAFNYTDHYLNSVLGRYDGNVYPKLFEEALKDPLNDSVVPDGYQEYLRPVLQQQLRTARL

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
F4JMK8F4JMK8_ARATHACX1651

Sequence caution

The sequence CAB10450.1 differs from that shown. Reason: Erroneous gene model prediction
The sequence CAB78718.1 differs from that shown. Reason: Erroneous gene model prediction

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF057044
EMBL· GenBank· DDBJ
AAC13498.1
EMBL· GenBank· DDBJ
mRNA
Z97341
EMBL· GenBank· DDBJ
CAB10450.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AL161544
EMBL· GenBank· DDBJ
CAB78718.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002687
EMBL· GenBank· DDBJ
AEE83798.1
EMBL· GenBank· DDBJ
Genomic DNA
AY058849
EMBL· GenBank· DDBJ
AAL24237.1
EMBL· GenBank· DDBJ
mRNA
BT001067
EMBL· GenBank· DDBJ
AAN46824.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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