O64437 · RINO1_ORYSJ
- ProteinInositol-3-phosphate synthase 1
- GeneRINO1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids510 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Key enzyme in myo-inositol biosynthesis pathway that catalyzes the conversion of glucose 6-phosphate to 1-myo-inositol 1-phosphate in a NAD-dependent manner (By similarity).
Is a key enzyme in the phytic acid biosynthesis pathway in seeds (PubMed:19021878).
Is a key enzyme in the phytic acid biosynthesis pathway in seeds (PubMed:19021878).
Miscellaneous
Plants silencing RINO1 show a low phytic acid phenotype in seeds, and high accumulation of inorganic phosphate (Pi) in seeds.
Catalytic activity
- D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate
Cofactor
Pathway
Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 1/2.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 70 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 71 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 72 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 73 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 143 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 180 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 190 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 193 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 230 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 231 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 232 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 233 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 281 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 282 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 306 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 309 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 340 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 341 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 342 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 355 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 393 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 394 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 422 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 423 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | nucleus | |
Molecular Function | inositol-3-phosphate synthase activity | |
Biological Process | inositol biosynthetic process | |
Biological Process | phospholipid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameInositol-3-phosphate synthase 1
- EC number
- Short namesMIP synthase 1
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionO64437
- Secondary accessions
Proteomes
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000195195 | 1-510 | UniProt | Inositol-3-phosphate synthase 1 | |||
Sequence: MFIESFRVESPHVRYGAAEIESDYQYDTTELVHESHDGASRWIVRPKSVRYNFRTTTTVPKLGVMLVGWGGNNGSTLTAGVIANREGISWATKDKVQQANYYGSLTQASTIRVGSYNGEEIYAPFKSLLPMVNPDDLVFGGWDISNMNLADAMTRAKVLDIDLQKQLRPYMESMVPLPGIYDPDFIAANQGSRANNVIKGTKKEQMEQIIKDIREFKEKSKVDKVVVLWTANTERYSNVCVGLNDTMENLLASVDKNEAEISPSTLYAIACVMEGIPFINGSPQNTFVPGLIDLAIKNNCLIGGDDFKSGQTKMKSVLVDFLVGAGIKPTSIVSYNHLGNNDGMNLSAPQTFRSKEISKSNVVDDMVSSNAILYELGEHPDHVVVIKYVPYVGDSKRAMDEYTSEIFMGGKSTIVLHNTCEDSLLAAPIILDLVLLAELSTRIQLKAEGEEKFHSFHPVATILSYLTKAPLVPPGTPVVNALAKQRAMLENIMRACVGLAPENNMILEYK | |||||||
Modified residue (large scale data) | 476 | PTMeXchange | Phosphothreonine | ||||
Sequence: T |
Proteomic databases
Expression
Tissue specificity
Highly expressed in embryos, but transcripts are undetectable in roots, leaves, flowers and anthers.
Induction
Induced by sucrose and glucose (PubMed:11975732).
Induced by abscisic acid (ABA) (PubMed:11975732, PubMed:24467907).
Induced by abscisic acid (ABA) (PubMed:11975732, PubMed:24467907).
Developmental stage
Expressed in the apical region of globular-stage embryos 2 days after anthesis (DAA). Expressed in the scutellum and aleurone layer 4 DAA. Expression in these cells increases until 7 DAA, and then gradually decreases.
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length510
- Mass (Da)56,264
- Last updated2006-05-16 v2
- Checksum20DD8DFAD199F937
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 185 | in Ref. 1; BAA25729 | ||||
Sequence: F → V |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB012107 EMBL· GenBank· DDBJ | BAA25729.1 EMBL· GenBank· DDBJ | mRNA | ||
EU267961 EMBL· GenBank· DDBJ | ACA50483.1 EMBL· GenBank· DDBJ | mRNA | ||
AC116604 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
DP000009 EMBL· GenBank· DDBJ | ABF94419.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP008209 EMBL· GenBank· DDBJ | BAF11156.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP014959 EMBL· GenBank· DDBJ | BAS82735.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CM000140 EMBL· GenBank· DDBJ | EEE58482.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK058750 EMBL· GenBank· DDBJ | BAG86795.1 EMBL· GenBank· DDBJ | mRNA | ||
AK103501 EMBL· GenBank· DDBJ | BAG96116.1 EMBL· GenBank· DDBJ | mRNA |