O62657 · SDF1_FELCA
- ProteinStromal cell-derived factor 1
- GeneCXCL12
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Plays a protective role after myocardial infarction. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 41-51 | heparin (UniProtKB | ChEBI) | |||
Site | 45 | Important for integrin interaction and activation | |||
Site | 46 | Important for dimer formation | |||
Site | 48 | Important for integrin interaction and activation | |||
Binding site | 62 | heparin (UniProtKB | ChEBI) | |||
Site | 64 | Important for integrin interaction and activation | |||
Binding site | 69 | heparin (UniProtKB | ChEBI) | |||
Binding site | 85 | heparin (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | external side of plasma membrane | |
Cellular Component | extracellular space | |
Molecular Function | chemokine activity | |
Molecular Function | chemokine receptor binding | |
Molecular Function | CXCR chemokine receptor binding | |
Molecular Function | growth factor activity | |
Molecular Function | integrin binding | |
Biological Process | axon guidance | |
Biological Process | cell chemotaxis | |
Biological Process | chemokine-mediated signaling pathway | |
Biological Process | defense response | |
Biological Process | immune response | |
Biological Process | induction of positive chemotaxis | |
Biological Process | integrin activation |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameStromal cell-derived factor 1
- Short namesSDF-1
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Carnivora > Feliformia > Felidae > Felinae > Felis
Accessions
- Primary accessionO62657
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain, disulfide bond.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Signal | 1-21 | ||||
Chain | PRO_0000005108 | 22-93 | Stromal cell-derived factor 1 | ||
Disulfide bond | 30↔55 | ||||
Disulfide bond | 32↔71 | ||||
Keywords
- PTM
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
Monomer or homodimer; in equilibrium. Dimer formation is induced by non acidic pH and the presence of multivalent anions, and by binding to CXCR4 or heparin. Monomeric form is required for full chemotactic activity and resistance to ischemia/reperfusion injury, whereas the dimeric form acts as a partial agonist of CXCR4, stimulating Ca2+ mobilization but with no chemotactic activity and instead acts as a selective antagonist that blocks chemotaxis induced by the monomeric form. Interacts with the N-terminus of ACKR3. Interacts with integrin subunit ITGB3 (via the allosteric site (site 2)). Interacts with TNFAIP6 (via Link domain).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for motif, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Motif | 22-23 | Receptor activation motif | |||
Region | 29-33 | Receptor and heparin binding | |||
Region | 39-41 | Receptor binding | |||
Region | 48-50 | Receptor binding | |||
Region | 60-70 | Receptor binding | |||
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 2 isoforms produced by Alternative splicing.
O62657-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameBeta
- SynonymsSDF-1b
- Length93
- Mass (Da)10,581
- Last updated1998-08-01 v1
- Checksum44FC763711E9BE37
O62657-2
- NameAlpha
- SynonymsSDF-1a
- Differences from canonical
- 90-93: Missing
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A5F5Y290 | A0A5F5Y290_FELCA | CXCL12 | 143 | ||
A0A2I2U9C3 | A0A2I2U9C3_FELCA | CXCL12 | 119 |
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Alternative sequence | VSP_001055 | 90-93 | in isoform Alpha | ||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB011965 EMBL· GenBank· DDBJ | BAA28601.1 EMBL· GenBank· DDBJ | mRNA | ||
AB011966 EMBL· GenBank· DDBJ | BAA28602.1 EMBL· GenBank· DDBJ | mRNA |