O61660 · TOP3_CAEEL
- ProteinDNA topoisomerase 3
- Genetop-3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids759 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Component of the BTR double Holliday Junction dissolution complex, which is involved in homologous recombination during meiotic double strand break in the germline (Probable). Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Catalytic activity
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 334 | O-(5'-phospho-DNA)-tyrosine intermediate | |||
Binding site | 716 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 718 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 743 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 753 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Cellular Component | RecQ family helicase-topoisomerase III complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA topoisomerase type I (single strand cut, ATP-independent) activity | |
Molecular Function | zinc ion binding | |
Biological Process | DNA recombination | |
Biological Process | DNA repair | |
Biological Process | DNA topological change |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA topoisomerase 3
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionO61660
- Secondary accessions
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000145194 | 1-759 | DNA topoisomerase 3 | ||
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
Component of the BTR double Holliday Junction dissolution complex composed of at least him-6, top-3, rmh-1 and rmif-2, which is involved in double strand break repair in the germline (Probable). May interact with rmh-1 (PubMed:27011106).
Complex viewer
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, zinc finger.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 3-147 | Toprim | |||
Domain | 165-590 | Topo IA-type catalytic | |||
Region | 609-715 | Disordered | |||
Zinc finger | 716-759 | GRF-type | |||
Sequence similarities
Belongs to the type IA topoisomerase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length759
- Mass (Da)85,438
- Last updated1998-08-01 v1
- Checksum3D862412D72946BD
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF057032 EMBL· GenBank· DDBJ | AAC13567.1 EMBL· GenBank· DDBJ | mRNA | ||
BX284603 EMBL· GenBank· DDBJ | CAB60518.2 EMBL· GenBank· DDBJ | Genomic DNA |