O61608 · NOS_ANOST

Function

function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Nitric oxide limits plasmodium development in the midgut.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
heme b (UniProtKB | Rhea| CHEBI:60344 )

FAD (UniProtKB | Rhea| CHEBI:57692 )

Note: Binds 1 FAD.
FMN (UniProtKB | Rhea| CHEBI:58210 )

Note: Binds 1 FMN.

Activity regulation

Stimulated by calcium/calmodulin.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site146(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin (UniProtKB | ChEBI)
Binding site224Fe (UniProtKB | ChEBI) of heme b (UniProtKB | ChEBI); axial binding residue
Binding site287L-arginine (UniProtKB | ChEBI)
Binding site396L-arginine (UniProtKB | ChEBI)
Binding site397L-arginine (UniProtKB | ChEBI)
Binding site401L-arginine (UniProtKB | ChEBI)
Binding site406L-arginine (UniProtKB | ChEBI)
Binding site487(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin (UniProtKB | ChEBI)
Binding site500(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin (UniProtKB | ChEBI)
Binding site515heme b (UniProtKB | ChEBI)
Binding site712-743FMN (UniProtKB | ChEBI)
Binding site855-866FAD (UniProtKB | ChEBI)
Binding site998-1008FAD (UniProtKB | ChEBI)
Binding site1073-1091NADP+ (UniProtKB | ChEBI)
Binding site1170-1185NADP+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functioncalmodulin binding
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular Functionheme binding
Molecular Functionmetal ion binding
Molecular FunctionNADP binding
Molecular Functionnitric-oxide synthase activity
Biological Processnitric oxide biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Nitric oxide synthase
  • EC number
  • Short names
    NOS

Organism names

Accessions

  • Primary accession
    O61608

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001709501-1247Nitric oxide synthase

Expression

Induction

Detected in the midgut and carcass soon after invasion of the midgut by plasmodium. Early induction is also primed by bacterial growth in the blood meal.

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region13-33Disordered
Region537-557Calmodulin-binding
Domain567-766Flavodoxin-like
Domain795-1065FAD-binding FR-type

Sequence similarities

Belongs to the NOS family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,247
  • Mass (Da)
    141,651
  • Last updated
    1999-01-01 v2
  • Checksum
    5F435E5EAC3D3778
MADTTTVVVERREVAEGRESSKANHIGEERRGYDVSRKRCSISVHGGGTEGGGGNMRTNYRELSPASLRIHRKSSHDIRNTLLGPDGEVLHLHDPSGKGGDGMGKMPAVVKPIKLKSIVTKAESYDTMHGKASDVMSCSREVCMGSVMTPHVIGTETRKPEIVQQHAKDFLDQYYSSIRRLKSPAHDSRWQQVQKEVEATGSYHLTETELIYGAKLAWRNSSRCIGRIQWSKLQVFDCRYVTTTSGMFEAICNHIKYATNKGNLRSAITIFPQRTDGKHDYRIWNNQIISYAGYKNADGKIIGDPANVEFTDFCVKLGWKSKRTEWDILPLVVSANGHDPDYFDYPPELILEVPLSHPQFKWFAELNLRWYAVPMVSSMLFDCGGIQFTATAFSGWYMSTEIGCRNLCDANRRNLLEPIAIKMGLDTRNPTSLWKDKALVEINIAVLHSYQSRNITIVDHHTASESFMKHFENETKLRNGCPADWIWIVPPMSASVTPVFHQEMAVYYLRPSFEYQESAMKTHIWKKGRDSAKNKKPRRKFNFKQIARAVKFTSKLFGRALSRRIKATVLYATETGRSEQYARQLVELLGHAFNAQIYCMSDYDISSIEHEALLLVVASTFGNGDPPENGELFAQDLYAMKLHESGHHQAHSELTIAASSKSFIKANSRSDLGKFGPMGGRKIDRLDSLRGSTTDTLSEETFGPLSNVRFAVFALGSSAYPNFCAFGKYIDNILGELGGERLMKMATGDEICGQEQAFRKWAPEVFKIACETFCLDPEETLSDAAFALQSELSENTVRYAPVAEYESLDRALSKFHNKKSMECSVKRNPINLHCEMNGTERSTILVEIMAEGIDYEPGDHVGIFPANRKEIVDGIIERLTGVNDPDEMLQLQVLKEKQTQNGVYKSWEPHERLPVCTLRTLLTRFLDITTPPTRQLLTYLASCCGDKADEERLLMLANESSVYEDWRYWKLPHLLEVLEEFPSCRPPAAVFVAQLNALQPRFYSISSSPRKYSNEIHLTVAIVTYRAEDGEGAEHYGVCSNYLANLQSDDKIYLFVRSAPSFHMSKDRTKPVILIGPGTGIAPFRSFWQEWDHIKTEMVDCKIPKVWLFFGCRTKNVDLYRDEKEEMVQHGVLDRVFLALSREENIPKTYVQDLALKEAESISELIMQEKGHIYVCGDVTMAEHVYQTLRKILATREKRTETEMEKYMLTLRDENRYHEDIFGITLRTAEIHNKSRATARIRMASQP

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF130134
EMBL· GenBank· DDBJ
AAC68577.1
EMBL· GenBank· DDBJ
Genomic DNA
AF130124
EMBL· GenBank· DDBJ
AAC68577.1
EMBL· GenBank· DDBJ
Genomic DNA
AF130125
EMBL· GenBank· DDBJ
AAC68577.1
EMBL· GenBank· DDBJ
Genomic DNA
AF130126
EMBL· GenBank· DDBJ
AAC68577.1
EMBL· GenBank· DDBJ
Genomic DNA
AF130127
EMBL· GenBank· DDBJ
AAC68577.1
EMBL· GenBank· DDBJ
Genomic DNA
AF130128
EMBL· GenBank· DDBJ
AAC68577.1
EMBL· GenBank· DDBJ
Genomic DNA
AF130129
EMBL· GenBank· DDBJ
AAC68577.1
EMBL· GenBank· DDBJ
Genomic DNA
AF130130
EMBL· GenBank· DDBJ
AAC68577.1
EMBL· GenBank· DDBJ
Genomic DNA
AF130131
EMBL· GenBank· DDBJ
AAC68577.1
EMBL· GenBank· DDBJ
Genomic DNA
AF130132
EMBL· GenBank· DDBJ
AAC68577.1
EMBL· GenBank· DDBJ
Genomic DNA
AF130133
EMBL· GenBank· DDBJ
AAC68577.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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