O61608 · NOS_ANOST
- ProteinNitric oxide synthase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1247 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Nitric oxide limits plasmodium development in the midgut.
Catalytic activity
- H+ + 2 L-arginine + 3 NADPH + 4 O2 = 4 H2O + 2 L-citrulline + 3 NADP+ + 2 nitric oxide
CHEBI:15378 + 2 CHEBI:32682 + 3 CHEBI:57783 + 4 CHEBI:15379 = 4 CHEBI:15377 + 2 CHEBI:57743 + 3 CHEBI:58349 + 2 CHEBI:16480
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 FAD.
Note: Binds 1 FMN.
Activity regulation
Stimulated by calcium/calmodulin.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 146 | (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 224 | Fe (UniProtKB | ChEBI) of heme b (UniProtKB | ChEBI); axial binding residue | ||||
Sequence: C | ||||||
Binding site | 287 | L-arginine (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 396 | L-arginine (UniProtKB | ChEBI) | ||||
Sequence: W | ||||||
Binding site | 397 | L-arginine (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 401 | L-arginine (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 406 | L-arginine (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 487 | (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin (UniProtKB | ChEBI) | ||||
Sequence: W | ||||||
Binding site | 500 | (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin (UniProtKB | ChEBI) | ||||
Sequence: F | ||||||
Binding site | 515 | heme b (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 712-743 | FMN (UniProtKB | ChEBI) | ||||
Sequence: VFALGSSAYPNFCAFGKYIDNILGELGGERLM | ||||||
Binding site | 855-866 | FAD (UniProtKB | ChEBI) | ||||
Sequence: YEPGDHVGIFPA | ||||||
Binding site | 998-1008 | FAD (UniProtKB | ChEBI) | ||||
Sequence: LQPRFYSISSS | ||||||
Binding site | 1073-1091 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: ILIGPGTGIAPFRSFWQEW | ||||||
Binding site | 1170-1185 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: KGHIYVCGDVTMAEHV |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | calmodulin binding | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | FMN binding | |
Molecular Function | heme binding | |
Molecular Function | metal ion binding | |
Molecular Function | NADP binding | |
Molecular Function | nitric-oxide synthase activity | |
Biological Process | nitric oxide biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNitric oxide synthase
- EC number
- Short namesNOS
Organism names
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Nematocera > Culicoidea > Culicidae > Anophelinae > Anopheles
Accessions
- Primary accessionO61608
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000170950 | 1-1247 | Nitric oxide synthase | |||
Sequence: MADTTTVVVERREVAEGRESSKANHIGEERRGYDVSRKRCSISVHGGGTEGGGGNMRTNYRELSPASLRIHRKSSHDIRNTLLGPDGEVLHLHDPSGKGGDGMGKMPAVVKPIKLKSIVTKAESYDTMHGKASDVMSCSREVCMGSVMTPHVIGTETRKPEIVQQHAKDFLDQYYSSIRRLKSPAHDSRWQQVQKEVEATGSYHLTETELIYGAKLAWRNSSRCIGRIQWSKLQVFDCRYVTTTSGMFEAICNHIKYATNKGNLRSAITIFPQRTDGKHDYRIWNNQIISYAGYKNADGKIIGDPANVEFTDFCVKLGWKSKRTEWDILPLVVSANGHDPDYFDYPPELILEVPLSHPQFKWFAELNLRWYAVPMVSSMLFDCGGIQFTATAFSGWYMSTEIGCRNLCDANRRNLLEPIAIKMGLDTRNPTSLWKDKALVEINIAVLHSYQSRNITIVDHHTASESFMKHFENETKLRNGCPADWIWIVPPMSASVTPVFHQEMAVYYLRPSFEYQESAMKTHIWKKGRDSAKNKKPRRKFNFKQIARAVKFTSKLFGRALSRRIKATVLYATETGRSEQYARQLVELLGHAFNAQIYCMSDYDISSIEHEALLLVVASTFGNGDPPENGELFAQDLYAMKLHESGHHQAHSELTIAASSKSFIKANSRSDLGKFGPMGGRKIDRLDSLRGSTTDTLSEETFGPLSNVRFAVFALGSSAYPNFCAFGKYIDNILGELGGERLMKMATGDEICGQEQAFRKWAPEVFKIACETFCLDPEETLSDAAFALQSELSENTVRYAPVAEYESLDRALSKFHNKKSMECSVKRNPINLHCEMNGTERSTILVEIMAEGIDYEPGDHVGIFPANRKEIVDGIIERLTGVNDPDEMLQLQVLKEKQTQNGVYKSWEPHERLPVCTLRTLLTRFLDITTPPTRQLLTYLASCCGDKADEERLLMLANESSVYEDWRYWKLPHLLEVLEEFPSCRPPAAVFVAQLNALQPRFYSISSSPRKYSNEIHLTVAIVTYRAEDGEGAEHYGVCSNYLANLQSDDKIYLFVRSAPSFHMSKDRTKPVILIGPGTGIAPFRSFWQEWDHIKTEMVDCKIPKVWLFFGCRTKNVDLYRDEKEEMVQHGVLDRVFLALSREENIPKTYVQDLALKEAESISELIMQEKGHIYVCGDVTMAEHVYQTLRKILATREKRTETEMEKYMLTLRDENRYHEDIFGITLRTAEIHNKSRATARIRMASQP |
Expression
Induction
Detected in the midgut and carcass soon after invasion of the midgut by plasmodium. Early induction is also primed by bacterial growth in the blood meal.
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 13-33 | Disordered | ||||
Sequence: EVAEGRESSKANHIGEERRGY | ||||||
Region | 537-557 | Calmodulin-binding | ||||
Sequence: PRRKFNFKQIARAVKFTSKLF | ||||||
Domain | 567-766 | Flavodoxin-like | ||||
Sequence: ATVLYATETGRSEQYARQLVELLGHAFNAQIYCMSDYDISSIEHEALLLVVASTFGNGDPPENGELFAQDLYAMKLHESGHHQAHSELTIAASSKSFIKANSRSDLGKFGPMGGRKIDRLDSLRGSTTDTLSEETFGPLSNVRFAVFALGSSAYPNFCAFGKYIDNILGELGGERLMKMATGDEICGQEQAFRKWAPEVF | ||||||
Domain | 795-1065 | FAD-binding FR-type | ||||
Sequence: NTVRYAPVAEYESLDRALSKFHNKKSMECSVKRNPINLHCEMNGTERSTILVEIMAEGIDYEPGDHVGIFPANRKEIVDGIIERLTGVNDPDEMLQLQVLKEKQTQNGVYKSWEPHERLPVCTLRTLLTRFLDITTPPTRQLLTYLASCCGDKADEERLLMLANESSVYEDWRYWKLPHLLEVLEEFPSCRPPAAVFVAQLNALQPRFYSISSSPRKYSNEIHLTVAIVTYRAEDGEGAEHYGVCSNYLANLQSDDKIYLFVRSAPSFHMS |
Sequence similarities
Belongs to the NOS family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,247
- Mass (Da)141,651
- Last updated1999-01-01 v2
- Checksum5F435E5EAC3D3778
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF130134 EMBL· GenBank· DDBJ | AAC68577.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF130124 EMBL· GenBank· DDBJ | AAC68577.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF130125 EMBL· GenBank· DDBJ | AAC68577.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF130126 EMBL· GenBank· DDBJ | AAC68577.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF130127 EMBL· GenBank· DDBJ | AAC68577.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF130128 EMBL· GenBank· DDBJ | AAC68577.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF130129 EMBL· GenBank· DDBJ | AAC68577.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF130130 EMBL· GenBank· DDBJ | AAC68577.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF130131 EMBL· GenBank· DDBJ | AAC68577.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF130132 EMBL· GenBank· DDBJ | AAC68577.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF130133 EMBL· GenBank· DDBJ | AAC68577.1 EMBL· GenBank· DDBJ | Genomic DNA |