O61309 · NOS_LYMST
- ProteinNitric oxide synthase
- GeneNOS
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1153 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body.
Catalytic activity
- H+ + 2 L-arginine + 3 NADPH + 4 O2 = 4 H2O + 2 L-citrulline + 3 NADP+ + 2 nitric oxide
CHEBI:15378 + 2 CHEBI:32682 + 3 CHEBI:57783 + 4 CHEBI:15379 = 4 CHEBI:15377 + 2 CHEBI:57743 + 3 CHEBI:58349 + 2 CHEBI:16480
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 FAD.
Note: Binds 1 FMN.
Activity regulation
Stimulated by calcium/calmodulin.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 3 | (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 82 | Fe (UniProtKB | ChEBI) of heme b (UniProtKB | ChEBI); axial binding residue | ||||
Sequence: C | ||||||
Binding site | 145 | L-arginine (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 254 | L-arginine (UniProtKB | ChEBI) | ||||
Sequence: W | ||||||
Binding site | 264 | L-arginine (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 358 | (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin (UniProtKB | ChEBI) | ||||
Sequence: F | ||||||
Binding site | 556-587 | FMN (UniProtKB | ChEBI) | ||||
Sequence: VFGLGSKAYPYYAAYGKYIYLMLQELGAERLV | ||||||
Binding site | 697-708 | FAD (UniProtKB | ChEBI) | ||||
Sequence: YAPGDHVAIFPA | ||||||
Binding site | 836-846 | FAD (UniProtKB | ChEBI) | ||||
Sequence: LQPRYYSISSS | ||||||
Binding site | 911-929 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: IMIGPGSGIAPFRSFWQQR | ||||||
Binding site | 1089-1104 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: GGHFYVSGDVSMAHDV |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | calmodulin binding | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | FMN binding | |
Molecular Function | heme binding | |
Molecular Function | metal ion binding | |
Molecular Function | NADP binding | |
Molecular Function | nitric-oxide synthase activity | |
Biological Process | nitric oxide biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNitric oxide synthase
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Spiralia > Lophotrochozoa > Mollusca > Gastropoda > Heterobranchia > Euthyneura > Panpulmonata > Hygrophila > Lymnaeoidea > Lymnaeidae > Lymnaea
Accessions
- Primary accessionO61309
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000170949 | 1-1153 | Nitric oxide synthase | |||
Sequence: MGSLSQQAHGPPDAPRSKEELLIHAKDFINQYFTSFQMNKTRAHFHRLGEINDLIEKSGTYDLTMAELTFGAKHAWRNAPGCIGRSQWSKLQVFDAREIGTPREMFEALCSHIRYATNEGKIRSTITIFPQRKEGRPDFRVWNTQLISYAGYKLGDGKVIGDPANVEFTEMCVEMGWKPKHGMFDLLPLVLSAAENSPEYFELPTELVLEVTLKHPEYPWFAEMGLKWYALPTDSGMLLDCGGLEFPSCPFNGWFMGTMIGSRNLCDPHRYNMLEPIGLKMGLNTETASSLWKDRVLIEVNVAVLYSFESANVTIVNHHDASTDFISHMDKEIKLRGGCPSDWVRMVPPMSGSTLEVFHQEMLLYNLHPAFVRQDVKPWKKHVWKSDQSVPINSCNPKRKLGFKALARAVEFSASLMSKALSSRVKCSIFYATETGRSERFARRLSEIFKPVFHSRVVCMDDYAVETLEHESLVMVITSTFGNGEPPENGKQFAQSLLDMKRKYDCDLGFLESCSSISTCIKSSILTEGPLAADVIGDRQSLAMGTGPLCNVRFAVFGLGSKAYPYYAAYGKYIYLMLQELGAERLVNYCAGDALYGQEQSFRAWSEEVFKASCEAFCLDNRNDAPGPQTKGDCSKVRIVPVENCQEPDLCQVLRNIHGKEVMPLILAERIQLQAKDSDQQTILIKLDAHNATDLKYAPGDHVAIFPANSPEIVDAILVRLDTSKGPSPDQVVKTEISTQLGTNDTWRSHLPICTSRTAFSFLLDVTTPPSQEILQVLATQASSDMDKHKLEQLASNSEAYEKWRLDLSPNILEILDEFPSLKIPPSLLLTQLPLLQPRYYSISSSQQKNPNEVHATIAVVRFKTQDGDGPVHEGVCSSWLNRSPIGTVVPCFLRSAPHFHLPEDPSLPIIMIGPGSGIAPFRSFWQQRLGEIENTMPSCENTMLSCETTIPSCENSMPSCENTMPSCENTMPSCENTIPSCENTIPSCENTMPSCENTIPSWERTMQPCQIILPSQTKKHFGEMVLYTGCRTAKHMIYAAELEEMKRLGVLSNYHVALSREAALPKMYVQDIIIKNAAAVYEIVMKKGGHFYVSGDVSMAHDVTRALELVLCQQGGREASQQVMSLRDENLFHEDIFGSFVRKAGGQRSEDE |
Structure
Family & Domains
Features
Showing features for region, domain, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 397-417 | Calmodulin-binding | ||||
Sequence: PKRKLGFKALARAVEFSASLM | ||||||
Domain | 427-610 | Flavodoxin-like | ||||
Sequence: CSIFYATETGRSERFARRLSEIFKPVFHSRVVCMDDYAVETLEHESLVMVITSTFGNGEPPENGKQFAQSLLDMKRKYDCDLGFLESCSSISTCIKSSILTEGPLAADVIGDRQSLAMGTGPLCNVRFAVFGLGSKAYPYYAAYGKYIYLMLQELGAERLVNYCAGDALYGQEQSFRAWSEEVF | ||||||
Domain | 660-903 | FAD-binding FR-type | ||||
Sequence: KEVMPLILAERIQLQAKDSDQQTILIKLDAHNATDLKYAPGDHVAIFPANSPEIVDAILVRLDTSKGPSPDQVVKTEISTQLGTNDTWRSHLPICTSRTAFSFLLDVTTPPSQEILQVLATQASSDMDKHKLEQLASNSEAYEKWRLDLSPNILEILDEFPSLKIPPSLLLTQLPLLQPRYYSISSSQQKNPNEVHATIAVVRFKTQDGDGPVHEGVCSSWLNRSPIGTVVPCFLRSAPHFHLP | ||||||
Repeat | 934-940 | 1 | ||||
Sequence: ENTMPSC | ||||||
Region | 934-1010 | 11 X 7 AA tandem repeats of E-[NTR]-[ST]-[IM]-[PLQ]-[SP]-[CW] | ||||
Sequence: ENTMPSCENTMLSCETTIPSCENSMPSCENTMPSCENTMPSCENTIPSCENTIPSCENTMPSCENTIPSWERTMQPC | ||||||
Repeat | 941-947 | 2 | ||||
Sequence: ENTMLSC | ||||||
Repeat | 948-954 | 3 | ||||
Sequence: ETTIPSC | ||||||
Repeat | 955-961 | 4 | ||||
Sequence: ENSMPSC | ||||||
Repeat | 962-968 | 5 | ||||
Sequence: ENTMPSC | ||||||
Repeat | 969-975 | 6 | ||||
Sequence: ENTMPSC | ||||||
Repeat | 976-982 | 7 | ||||
Sequence: ENTIPSC | ||||||
Repeat | 983-989 | 8 | ||||
Sequence: ENTIPSC | ||||||
Repeat | 990-996 | 9 | ||||
Sequence: ENTMPSC | ||||||
Repeat | 997-1003 | 10 | ||||
Sequence: ENTIPSW | ||||||
Repeat | 1004-1010 | 11 | ||||
Sequence: ERTMQPC |
Sequence similarities
Belongs to the NOS family.
Keywords
- Domain
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
O61309-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameLong
- Length1,153
- Mass (Da)129,086
- Last updated1998-08-01 v1
- Checksum101B77D02B66B109
O61309-2
- NameShort
- Differences from canonical
- 276-309: Missing
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_003584 | 276-309 | in isoform Short | |||
Sequence: Missing |
Keywords
- Coding sequence diversity