O60763 · USO1_HUMAN
- ProteinGeneral vesicular transport factor p115
- GeneUSO1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids962 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | endoplasmic reticulum | |
Cellular Component | ER to Golgi transport vesicle membrane | |
Cellular Component | fibrillar center | |
Cellular Component | Golgi apparatus | |
Cellular Component | Golgi membrane | |
Cellular Component | Golgi stack | |
Cellular Component | membrane | |
Cellular Component | microtubule organizing center | |
Cellular Component | perinuclear region of cytoplasm | |
Cellular Component | transport vesicle | |
Molecular Function | cadherin binding | |
Molecular Function | RNA binding | |
Biological Process | endoplasmic reticulum to Golgi vesicle-mediated transport | |
Biological Process | Golgi vesicle docking | |
Biological Process | intracellular protein transport | |
Biological Process | membrane fusion | |
Biological Process | regulation of cellular response to insulin stimulus | |
Biological Process | secretory granule localization | |
Biological Process | small GTPase-mediated signal transduction | |
Biological Process | transcytosis | |
Biological Process | vesicle fusion with Golgi apparatus |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGeneral vesicular transport factor p115
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionO60763
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Peripheral membrane protein
Note: Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.
Keywords
- Cellular component
Disease & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 942 | Loss of phosphorylation. Promotes association with Golgi membranes. | ||||
Sequence: S → A | ||||||
Mutagenesis | 942 | Decreased association with Golgi membranes. | ||||
Sequence: S → D |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 950 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000065774 | 1-962 | UniProt | General vesicular transport factor p115 | |||
Sequence: MNFLRGVMGGQSAGPQHTEAETIQKLCDRVASSTLLDDRRNAVRALKSLSKKYRLEVGIQAMEHLIHVLQTDRSDSEIIGYALDTLYNIISNEEEEEVEENSTRQSEDLGSQFTEIFIKQQENVTLLLSLLEEFDFHVRWPGVKLLTSLLKQLGPQVQQIILVSPMGVSRLMDLLADSREVIRNDGVLLLQALTRSNGAIQKIVAFENAFERLLDIISEEGNSDGGIVVEDCLILLQNLLKNNNSNQNFFKEGSYIQRMKPWFEVGDENSGWSAQKVTNLHLMLQLVRVLVSPTNPPGATSSCQKAMFQCGLLQQLCTILMATGVPADILTETINTVSEVIRGCQVNQDYFASVNAPSNPPRPAIVVLLMSMVNERQPFVLRCAVLYCFQCFLYKNQKGQGEIVSTLLPSTIDATGNSVSAGQLLCGGLFSTDSLSNWCAAVALAHALQENATQKEQLLRVQLATSIGNPPVSLLQQCTNILSQGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNSLESYMKEKLKQLIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTLEQHDNIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRLREEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAFAKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLADQDQKILSLKNKLKDLGHPVEEEDELESGDQEDEDDESEDPGKDLDHI | |||||||
Modified residue | 50 | UniProt | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue | 202 | UniProt | N6-acetyllysine | ||||
Sequence: K | |||||||
Modified residue (large scale data) | 717 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 851 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue | 942 | UniProt | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 942 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue | 952 | UniProt | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 952 | PRIDE | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Homodimer. Dimerizes by parallel association of the tails, resulting in an elongated structure with two globular head domains side by side, and a long rod-like tail structure (Probable). Interacts with MIF.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | O60763 | AKAP9 Q99996-2 | 3 | EBI-356164, EBI-9641546 | |
BINARY | O60763 | DUSP12 Q9UNI6 | 3 | EBI-356164, EBI-715161 | |
XENO | O60763 | N P0DTC9 | 4 | EBI-356164, EBI-25475856 | |
BINARY | O60763 | PHYKPL Q8IUZ5 | 3 | EBI-356164, EBI-751947 | |
BINARY | O60763 | SETD9 Q8NE22 | 2 | EBI-356164, EBI-10698970 | |
BINARY | O60763 | XPO7 Q9UIA9 | 3 | EBI-356164, EBI-286668 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, repeat, coiled coil, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-637 | Globular head | ||||
Sequence: MNFLRGVMGGQSAGPQHTEAETIQKLCDRVASSTLLDDRRNAVRALKSLSKKYRLEVGIQAMEHLIHVLQTDRSDSEIIGYALDTLYNIISNEEEEEVEENSTRQSEDLGSQFTEIFIKQQENVTLLLSLLEEFDFHVRWPGVKLLTSLLKQLGPQVQQIILVSPMGVSRLMDLLADSREVIRNDGVLLLQALTRSNGAIQKIVAFENAFERLLDIISEEGNSDGGIVVEDCLILLQNLLKNNNSNQNFFKEGSYIQRMKPWFEVGDENSGWSAQKVTNLHLMLQLVRVLVSPTNPPGATSSCQKAMFQCGLLQQLCTILMATGVPADILTETINTVSEVIRGCQVNQDYFASVNAPSNPPRPAIVVLLMSMVNERQPFVLRCAVLYCFQCFLYKNQKGQGEIVSTLLPSTIDATGNSVSAGQLLCGGLFSTDSLSNWCAAVALAHALQENATQKEQLLRVQLATSIGNPPVSLLQQCTNILSQGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNSLESYMKEKLKQLIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKK | ||||||
Repeat | 20-60 | ARM 1 | ||||
Sequence: AETIQKLCDRVASSTLLDDRRNAVRALKSLSKKYRLEVGIQ | ||||||
Repeat | 61-121 | ARM 2 | ||||
Sequence: AMEHLIHVLQTDRSDSEIIGYALDTLYNIISNEEEEEVEENSTRQSEDLGSQFTEIFIKQQ | ||||||
Repeat | 123-163 | ARM 3 | ||||
Sequence: NVTLLLSLLEEFDFHVRWPGVKLLTSLLKQLGPQVQQIILV | ||||||
Repeat | 166-207 | ARM 4 | ||||
Sequence: MGVSRLMDLLADSREVIRNDGVLLLQALTRSNGAIQKIVAFE | ||||||
Repeat | 208-253 | ARM 5 | ||||
Sequence: NAFERLLDIISEEGNSDGGIVVEDCLILLQNLLKNNNSNQNFFKEG | ||||||
Repeat | 255-310 | ARM 6 | ||||
Sequence: YIQRMKPWFEVGDENSGWSAQKVTNLHLMLQLVRVLVSPTNPPGATSSCQKAMFQC | ||||||
Repeat | 311-354 | ARM 7 | ||||
Sequence: GLLQQLCTILMATGVPADILTETINTVSEVIRGCQVNQDYFASV | ||||||
Repeat | 363-408 | ARM 8 | ||||
Sequence: PAIVVLLMSMVNERQPFVLRCAVLYCFQCFLYKNQKGQGEIVSTLL | ||||||
Repeat | 420-459 | ARM 9 | ||||
Sequence: SAGQLLCGGLFSTDSLSNWCAAVALAHALQENATQKEQLL | ||||||
Repeat | 473-513 | ARM 10 | ||||
Sequence: SLLQQCTNILSQGSKIQTRVGLLMLLCTWLSNCPIAVTHFL | ||||||
Repeat | 518-571 | ARM 11 | ||||
Sequence: NVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNSLESYMKEKLKQLIEKR | ||||||
Repeat | 573-630 | ARM 12 | ||||
Sequence: GKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYK | ||||||
Coiled coil | 638-930 | |||||
Sequence: EEEVKKTLEQHDNIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRLREEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAFAKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLADQDQKILSLKNKLKDL | ||||||
Compositional bias | 763-782 | Polar residues | ||||
Sequence: MKSSQTSGTNEQSSAIVSAR | ||||||
Region | 763-783 | Disordered | ||||
Sequence: MKSSQTSGTNEQSSAIVSARD | ||||||
Region | 926-962 | Disordered | ||||
Sequence: KLKDLGHPVEEEDELESGDQEDEDDESEDPGKDLDHI | ||||||
Compositional bias | 936-955 | Acidic residues | ||||
Sequence: EEDELESGDQEDEDDESEDP |
Domain
Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
Sequence similarities
Belongs to the VDP/USO1/EDE1 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
O60763-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length962
- Mass (Da)107,895
- Last updated2007-03-06 v2
- ChecksumC963652209031008
O60763-2
- Name2
Sequence caution
Features
Showing features for sequence conflict, alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 75 | in Ref. 3; CAD89917 | ||||
Sequence: D → G | ||||||
Sequence conflict | 85 | in Ref. 1; BAA25300 | ||||
Sequence: T → I | ||||||
Sequence conflict | 93 | in Ref. 5; AAH32654 | ||||
Sequence: Missing | ||||||
Alternative sequence | VSP_039120 | 98 | in isoform 2 | |||
Sequence: V → VDDVE | ||||||
Sequence conflict | 248 | in Ref. 3; CAD89917 | ||||
Sequence: N → Y | ||||||
Alternative sequence | VSP_039121 | 484 | in isoform 2 | |||
Sequence: Q → QGDKIDRR | ||||||
Sequence conflict | 650 | in Ref. 3; CAD89917 | ||||
Sequence: N → D | ||||||
Compositional bias | 763-782 | Polar residues | ||||
Sequence: MKSSQTSGTNEQSSAIVSAR | ||||||
Sequence conflict | 877 | in Ref. 2; BAG36947 | ||||
Sequence: Q → R | ||||||
Compositional bias | 936-955 | Acidic residues | ||||
Sequence: EEDELESGDQEDEDDESEDP |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D86326 EMBL· GenBank· DDBJ | BAA25300.1 EMBL· GenBank· DDBJ | mRNA | ||
AK314289 EMBL· GenBank· DDBJ | BAG36947.1 EMBL· GenBank· DDBJ | mRNA | ||
AL832010 EMBL· GenBank· DDBJ | CAD89917.1 EMBL· GenBank· DDBJ | mRNA | ||
AC110615 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AC104828 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC006398 EMBL· GenBank· DDBJ | AAH06398.1 EMBL· GenBank· DDBJ | mRNA | Sequence problems. | |
BC032654 EMBL· GenBank· DDBJ | AAH32654.1 EMBL· GenBank· DDBJ | mRNA |