O60507 · TPST1_HUMAN
- ProteinProtein-tyrosine sulfotransferase 1
- GeneTPST1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids370 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.
Catalytic activity
- 3'-phosphoadenylyl sulfate + L-tyrosyl-[protein] = adenosine 3',5'-bisphosphate + H+ + O-sulfo-L-tyrosine-[protein]
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 79-83 | 3'-phosphoadenylyl sulfate (UniProtKB | ChEBI) | ||||
Sequence: RSGTT | ||||||
Active site | 100 | Proton donor/acceptor | ||||
Sequence: E | ||||||
Site | 159 | Transition state stabilizer | ||||
Sequence: K | ||||||
Binding site | 184 | 3'-phosphoadenylyl sulfate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 192 | 3'-phosphoadenylyl sulfate (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 196 | 3'-phosphoadenylyl sulfate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 239 | 3'-phosphoadenylyl sulfate (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Site | 286 | Transition state stabilizer | ||||
Sequence: S | ||||||
Binding site | 286-295 | 3'-phosphoadenylyl sulfate (UniProtKB | ChEBI) | ||||
Sequence: STDQVIKPVN | ||||||
Binding site | 301 | 3'-phosphoadenylyl sulfate (UniProtKB | ChEBI) | ||||
Sequence: K |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | Golgi apparatus | |
Cellular Component | Golgi membrane | |
Cellular Component | membrane | |
Molecular Function | protein homodimerization activity | |
Molecular Function | protein-tyrosine sulfotransferase activity | |
Biological Process | 3'-phosphoadenosine 5'-phosphosulfate metabolic process | |
Biological Process | post-translational protein modification |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein-tyrosine sulfotransferase 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionO60507
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Single-pass type II membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-8 | Cytoplasmic | ||||
Sequence: MVGKLKQN | ||||||
Transmembrane | 9-25 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: LLLACLVISSVTVFYLG | ||||||
Topological domain | 26-370 | Lumenal | ||||
Sequence: QHAMECHHRIEERSQPVKLESTRTTVRTGLDLKANKTFAYHKDMPLIFIGGVPRSGTTLMRAMLDAHPDIRCGEETRVIPRILALKQMWSRSSKEKIRLDEAGVTDEVLDSAMQAFLLEIIVKHGEPAPYLCNKDPFALKSLTYLSRLFPNAKFLLMVRDGRASVHSMISRKVTIAGFDLNSYRDCLTKWNRAIETMYNQCMEVGYKKCMLVHYEQLVLHPERWMRTLLKFLQIPWNHSVLHHEEMIGKAGGVSLSKVERSTDQVIKPVNVGALSKWVGKIPPDVLQDMAVIAPMLAKLGYDPYANPPNYGKPDPKIIENTRRVYKGEFQLPDFLKEKPQTEQVE |
Keywords
- Cellular component
Disease & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 60 | Loss of one glycosylation site. Loss of N-glycosylation; when associated with A-262. | ||||
Sequence: N → A | ||||||
Mutagenesis | 262 | Loss of one glycosylation site. Loss of N-glycosylation; when associated with A-60. | ||||
Sequence: N → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 355 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Genetic variation databases
PTM/Processing
Features
Showing features for chain, glycosylation, disulfide bond, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000189826 | 1-370 | UniProt | Protein-tyrosine sulfotransferase 1 | |||
Sequence: MVGKLKQNLLLACLVISSVTVFYLGQHAMECHHRIEERSQPVKLESTRTTVRTGLDLKANKTFAYHKDMPLIFIGGVPRSGTTLMRAMLDAHPDIRCGEETRVIPRILALKQMWSRSSKEKIRLDEAGVTDEVLDSAMQAFLLEIIVKHGEPAPYLCNKDPFALKSLTYLSRLFPNAKFLLMVRDGRASVHSMISRKVTIAGFDLNSYRDCLTKWNRAIETMYNQCMEVGYKKCMLVHYEQLVLHPERWMRTLLKFLQIPWNHSVLHHEEMIGKAGGVSLSKVERSTDQVIKPVNVGALSKWVGKIPPDVLQDMAVIAPMLAKLGYDPYANPPNYGKPDPKIIENTRRVYKGEFQLPDFLKEKPQTEQVE | |||||||
Glycosylation | 60 | UniProt | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | |||||||
Disulfide bond | 97↔157 | UniProt | |||||
Sequence: CGEETRVIPRILALKQMWSRSSKEKIRLDEAGVTDEVLDSAMQAFLLEIIVKHGEPAPYLC | |||||||
Disulfide bond | 226↔234 | UniProt | |||||
Sequence: CMEVGYKKC | |||||||
Glycosylation | 262 | UniProt | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | |||||||
Modified residue (large scale data) | 279 | PRIDE | Phosphoserine | ||||
Sequence: S |
Post-translational modification
N-glycosylated.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Gene expression databases
Organism-specific databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 102-106 | Interaction with peptide substrate | ||||
Sequence: RVIPR |
Sequence similarities
Belongs to the protein sulfotransferase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length370
- Mass (Da)42,188
- Last updated1998-08-01 v1
- ChecksumFB9EB341AFE36407
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF038009 EMBL· GenBank· DDBJ | AAC13552.1 EMBL· GenBank· DDBJ | mRNA | ||
AK313098 EMBL· GenBank· DDBJ | BAG35922.1 EMBL· GenBank· DDBJ | mRNA | ||
CR542060 EMBL· GenBank· DDBJ | CAG46857.1 EMBL· GenBank· DDBJ | mRNA | ||
CR542080 EMBL· GenBank· DDBJ | CAG46877.1 EMBL· GenBank· DDBJ | mRNA | ||
CH236961 EMBL· GenBank· DDBJ | EAL23739.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CH471140 EMBL· GenBank· DDBJ | EAX07937.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC013188 EMBL· GenBank· DDBJ | AAH13188.1 EMBL· GenBank· DDBJ | mRNA |