O60356 · NUPR1_HUMAN

  • Protein
    Nuclear protein 1
  • Gene
    NUPR1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Transcription regulator that converts stress signals into a program of gene expression that empowers cells with resistance to the stress induced by a change in their microenvironment. Thereby participates in regulation of many process namely cell-cycle, apoptosis, autophagy and DNA repair responses (PubMed:11056169, PubMed:11940591, PubMed:16300740, PubMed:16478804, PubMed:18690848, PubMed:19650074, PubMed:19723804, PubMed:20181828, PubMed:22565310, PubMed:22858377, PubMed:30451898).
Controls cell cycle progression and protects cells from genotoxic stress induced by doxorubicin through the complex formation with TP53 and EP300 that binds CDKN1A promoter leading to transcriptional induction of CDKN1A (PubMed:18690848).
Protects pancreatic cancer cells from stress-induced cell death by binding the RELB promoter and activating its transcription, leading to IER3 transactivation (PubMed:22565310).
Negatively regulates apoptosis through interaction with PTMA (PubMed:16478804).
Inhibits autophagy-induced apoptosis in cardiac cells through FOXO3 interaction, inducing cytoplasmic translocation of FOXO3 thereby preventing the FOXO3 association with the pro-autophagic BNIP3 promoter (PubMed:20181828).
Inhibits cell growth and facilitates programmed cell death by apoptosis after adriamycin-induced DNA damage through transactivation of TP53 (By similarity).
Regulates methamphetamine-induced apoptosis and autophagy through DDIT3-mediated endoplasmic reticulum stress pathway (By similarity).
Participates in DNA repair following gamma-irradiation by facilitating DNA access of the transcription machinery through interaction with MSL1 leading to inhibition of histone H4' Lys-16' acetylation (H4K16ac) (PubMed:19650074).
Coactivator of PAX2 transcription factor activity, both by recruiting EP300 to increase PAX2 transcription factor activity and by binding PAXIP1 to suppress PAXIP1-induced inhibition on PAX2 (PubMed:11940591).
Positively regulates cell cycle progression through interaction with COPS5 inducing cytoplasmic translocation of CDKN1B leading to the CDKN1B degradation (PubMed:16300740).
Coordinates, through its interaction with EP300, the assiociation of MYOD1, EP300 and DDX5 to the MYOG promoter, leading to inhibition of cell-cycle progression and myogenic differentiation promotion (PubMed:19723804).
Negatively regulates beta cell proliferation via inhibition of cell-cycle regulatory genes expression through the suppression of their promoter activities (By similarity).
Also required for LHB expression and ovarian maturation (By similarity).
Exacerbates CNS inflammation and demyelination upon cuprizone treatment (By similarity).

Miscellaneous

Mediates resistance to anticancer drugs, namely taxol, doxorubicin, gemcitabine.

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentintercellular bridge
Cellular Componentnucleoplasm
Cellular Componentnucleus
Cellular Componentperinuclear region of cytoplasm
Cellular Componentprotein-DNA complex
Molecular Functionacetyltransferase activator activity
Molecular Functionchromatin binding
Molecular FunctionDNA binding
Molecular Functiontranscription coactivator activity
Biological Processacute inflammatory response
Biological Processfibroblast apoptotic process
Biological Processfibroblast proliferation
Biological Processintrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
Biological Processmale gonad development
Biological Processnegative regulation of apoptotic process
Biological Processnegative regulation of autophagosome assembly
Biological Processnegative regulation of autophagy
Biological Processnegative regulation of cardiac muscle cell apoptotic process
Biological Processnegative regulation of cell cycle
Biological Processnegative regulation of cell population proliferation
Biological Processnegative regulation of DNA-binding transcription factor activity
Biological Processnegative regulation of epithelial cell apoptotic process
Biological Processnegative regulation of epithelial cell proliferation
Biological Processnegative regulation of fibroblast proliferation
Biological Processnegative regulation of glycolytic process
Biological Processnegative regulation of programmed necrotic cell death
Biological Processnegative regulation of type B pancreatic cell proliferation
Biological Processpositive regulation of cell cycle
Biological Processpositive regulation of fibroblast apoptotic process
Biological Processpositive regulation of intrinsic apoptotic signaling pathway
Biological Processpositive regulation of neuroinflammatory response
Biological Processpositive regulation of neuron apoptotic process
Biological Processpositive regulation of oxidative phosphorylation
Biological Processpositive regulation of proteasomal protein catabolic process
Biological Processpositive regulation of protein modification process
Biological Processprotein-containing complex assembly
Biological Processregulation of autophagy
Biological Processregulation of female gonad development
Biological Processregulation of response to endoplasmic reticulum stress
Biological Processresponse to toxic substance
Biological Processskeletal muscle cell differentiation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Nuclear protein 1
  • Alternative names
    • Candidate of metastasis 1
    • Protein p8

Gene names

    • Name
      NUPR1
    • Synonyms
      COM1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo

Accessions

  • Primary accession
    O60356
  • Secondary accessions
    • B2R5C4
    • O60357
    • Q6FGG3

Proteomes

Organism-specific databases

Disease & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis33Impairs interaction with RNF2.
Mutagenesis68Impairs interaction with RNF2.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 124 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Organism-specific databases

Miscellaneous

Genetic variation databases

PTM/Processing

Features

Showing features for chain, modified residue (large scale data).

TypeIDPosition(s)SourceDescription
ChainPRO_00000580071-82UniProtNuclear protein 1
Modified residue (large scale data)22PRIDEPhosphoserine
Modified residue (large scale data)23PRIDEPhosphoserine
Modified residue (large scale data)58PRIDEPhosphoserine

Post-translational modification

Phosphorylated in vitro by PKA and CK. Phosphorylation promotes DNA-binding activity.
Acetylated by EP300 in vitro.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Widely expressed, with high levels in liver, pancreas, prostate, ovary, colon, thyroid, spinal cord, trachea and adrenal gland, moderate levels in heart, placenta, lung, skeletal muscle, kidney, testis, small intestine, stomach and lymph node, and low levels in brain, spleen, thymus and bone marrow. Not detected in peripheral blood leukocytes.

Induction

Up-regulated by stress agents, such as nutrient deprivation (at protein level) (PubMed:22565310).
Up-regulation by gamma-irradiation is eventually followed by down-regulation (PubMed:19650074).
Expression increases with the tumor aggressiveness. up-regulated by DNA-damaging agent such as doxorubicin (PubMed:18690848).

Gene expression databases

Organism-specific databases

Interaction

Subunit

Monomer. Directly interacts with MSL1 and binds MORF4L1, two components of histone acetyltransferase complex; the interaction with MORF4L1 may be mediated by MSL1 (PubMed:19650074).
Interacts with EP300; this interaction enhances the effect of EP300 on PAX2 transcription factor activity (PubMed:11940591).
Interacts with PAXIP1; this interaction prevents PAXIP1 inhibition of PAX2 transcription factor activity (PubMed:11940591).
Interacts with COPS5; this interaction allows COPS5-dependent CDKN1B nuclear to cytoplasm translocation (PubMed:16300740).
Interacts with RNF2 (PubMed:28720707).
Interacts with FOXO3; this interaction represses FOXO3 transactivation (PubMed:20181828).
Interacts with PTMA; negatively regulates apoptotic process (PubMed:16478804).
Interacts with MYOD1, EP300 and DDX5; this interaction coordinates the association of anti-proliferative and pro-myogenic proteins at the myogenin promoter (By similarity) (PubMed:19723804).
Interacts with TP53; interaction is stress-dependent (PubMed:18690848).
Forms a complex with EP300 and TP53; this complex binds CDKN1A promoter leading to transcriptional induction of CDKN1A (PubMed:18690848).

Binary interactions

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region, motif.

TypeIDPosition(s)Description
Region1-82Disordered
Motif65-82Nuclear localization signal

Sequence similarities

Belongs to the NUPR family.

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

O60356-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    82
  • Mass (Da)
    8,873
  • Last updated
    1998-08-01 v1
  • Checksum
    596E342D10F87AF3
MATFPPATSAPQQPPGPEDEDSSLDESDLYSLAHSYLGGGGRKGRTKREAAANTNRPSPGGHERKLVTKLQNSERKKRGARR

O60356-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 37-37: L → LGPLIMPMPTSPLTPALVT

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
H3BS92H3BS92_HUMANNUPR143

Sequence caution

The sequence AAC05335.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for alternative sequence, sequence conflict.

TypeIDPosition(s)Description
Alternative sequenceVSP_05381637in isoform 2
Sequence conflict47in Ref. 3; BAG35071

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF069073
EMBL· GenBank· DDBJ
AAC19384.1
EMBL· GenBank· DDBJ
mRNA
AF069074
EMBL· GenBank· DDBJ
AAC19385.1
EMBL· GenBank· DDBJ
Genomic DNA
AF135266
EMBL· GenBank· DDBJ
AAD49221.1
EMBL· GenBank· DDBJ
mRNA
AK312135
EMBL· GenBank· DDBJ
BAG35071.1
EMBL· GenBank· DDBJ
mRNA
BM792961
EMBL· GenBank· DDBJ
-mRNA No translation available.
BT006896
EMBL· GenBank· DDBJ
AAP35542.1
EMBL· GenBank· DDBJ
mRNA
CR542144
EMBL· GenBank· DDBJ
CAG46941.1
EMBL· GenBank· DDBJ
mRNA
AC002425
EMBL· GenBank· DDBJ
AAC05336.1
EMBL· GenBank· DDBJ
Genomic DNA
AC002425
EMBL· GenBank· DDBJ
AAC05335.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CH471279
EMBL· GenBank· DDBJ
EAW52274.1
EMBL· GenBank· DDBJ
Genomic DNA
BC002434
EMBL· GenBank· DDBJ
AAH02434.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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