O57404 · O57404_PLEWA

Function

function

The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the reticulum endoplasmic. Once cleaved, ShhC is degraded in the endoplasmic reticulum.

Protein hedgehog N-product

The dually lipidated hedgehog protein N-product is a morphogen which is essential for a variety of patterning events during development.

Protein hedgehog

The C-terminal part of the hedgehog protein precursor displays an autoproteolysis activity that results in the cleavage of the full-length protein into two parts (N-product and C-product). In addition, the C-terminal part displays a cholesterol transferase activity that results by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product.

Catalytic activity

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site92Ca2+ 1 (UniProtKB | ChEBI)
Binding site93Ca2+ 1 (UniProtKB | ChEBI)
Binding site93Ca2+ 2 (UniProtKB | ChEBI)
Binding site98Ca2+ 1 (UniProtKB | ChEBI)
Binding site128Ca2+ 1 (UniProtKB | ChEBI)
Binding site129Ca2+ 2 (UniProtKB | ChEBI)
Binding site129Ca2+ 1 (UniProtKB | ChEBI)
Binding site132Ca2+ 2 (UniProtKB | ChEBI)
Binding site134Ca2+ 2 (UniProtKB | ChEBI)
Binding site143Zn2+ (UniProtKB | ChEBI)
Binding site150Zn2+ (UniProtKB | ChEBI)
Binding site185Zn2+ (UniProtKB | ChEBI)
Site200-201Cleavage; by autolysis
Site246Involved in cholesterol transfer
Site270Involved in auto-cleavage
Site273Essential for auto-cleavage

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Cellular Componentextracellular space
Cellular ComponentGolgi membrane
Cellular Componentplasma membrane
Molecular Functioncalcium ion binding
Molecular Functionpatched binding
Molecular Functionpeptidase activity
Molecular Functiontransferase activity
Biological Processanimal organ development
Biological Processcell development
Biological Processcell fate specification
Biological Processcell-cell signaling
Biological Processcentral nervous system development
Biological Processintein-mediated protein splicing
Biological Processneuron differentiation
Biological Processpattern specification process
Biological Processprotein autoprocessing
Biological Processregulation of cell population proliferation
Biological Processregulation of developmental process
Biological Processregulation of gene expression
Biological Processsmoothened signaling pathway
Biological Processtissue development
Biological Processtube development

Keywords

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Hedgehog protein

Gene names

    • Name
      Pw-shh

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Caudata > Salamandroidea > Salamandridae > Pleurodelinae > Pleurodeles

Accessions

  • Primary accession
    O57404

Subcellular Location

Endoplasmic reticulum membrane

Sonic hedgehog protein

Endoplasmic reticulum membrane
Golgi apparatus membrane

Protein hedgehog N-product

Cell membrane
; Lipid-anchor

Keywords

PTM/Processing

Keywords

Interaction

Subunit

Multimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain199-317Hint
Domain320-364Hint

Sequence similarities

Belongs to the hedgehog family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    434
  • Mass (Da)
    48,421
  • Last updated
    1998-06-01 v1
  • Checksum
    A9495E367151AE74
MGEMILLRRDLLGGFISTLLVPLGLGCGPGRGIGQRRRPQKLTPLAYKQFIPNVSEKTLGASGRYEVKITRNSERFQELTPNYNPDIIFKVEENTGADRLMTQRCKDKLNSLAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYFESKAHIHCSVKAENSVAVKSGGCFPGSATVTLEQGVRIPVKGLKPGNRVLAVDVEGRLIYSDFLLFMDEEETARKVFYVIETSLPRERLRLTAAHLLFVAQEHPGNASAGNFRSKFGHRRFRSMFASSVRPGHRVLTEDREGRGLREATVDRVYLEEATGAYAPVTAHGTVVIDRVLASCYAVIEEHSWAHWAFAPLRVGFGILSFFSPQDYSSHSPPAPSQAEGVHWYSEILYRIGTWVLQADTIHPLGMAAKSS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF003532
EMBL· GenBank· DDBJ
AAB94412.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp