O55012 · PICAL_RAT

  • Protein
    Phosphatidylinositol-binding clathrin assembly protein
  • Gene
    Picalm
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcell surface
Cellular Componentclathrin coat of coated pit
Cellular Componentclathrin-coated pit
Cellular Componentclathrin-coated vesicle
Cellular Componentearly endosome
Cellular Componentendosome
Cellular Componentendosome to plasma membrane transport vesicle
Cellular Componentextrinsic component of presynaptic endocytic zone membrane
Cellular ComponentGolgi apparatus
Cellular Componentmembrane
Cellular Componentneurofibrillary tangle
Cellular Componentneuronal cell body
Cellular Componentnucleus
Cellular Componentparallel fiber to Purkinje cell synapse
Cellular Componentperinuclear region of cytoplasm
Cellular Componentplasma membrane
Cellular Componentpostsynapse
Cellular Componentpostsynaptic density
Cellular Componentpostsynaptic endocytic zone
Cellular Componentpostsynaptic membrane
Cellular Componentpresynaptic membrane
Cellular ComponentSchaffer collateral - CA1 synapse
Cellular Componentsynaptic vesicle
Cellular Componentvesicle
Molecular Function1-phosphatidylinositol binding
Molecular Functionclathrin binding
Molecular Functionclathrin heavy chain binding
Molecular Functionidentical protein binding
Molecular Functionlow-density lipoprotein particle receptor binding
Molecular Functionphosphatidylinositol-4,5-bisphosphate binding
Molecular FunctionSH3 domain binding
Molecular Functionsmall GTPase binding
Molecular FunctionSNARE binding
Molecular Functiontau protein binding
Biological Processamyloid-beta clearance by transcytosis
Biological Processaxonogenesis
Biological Processclathrin coat assembly
Biological Processclathrin-dependent endocytosis
Biological Processdendrite morphogenesis
Biological Processendocytosis
Biological Processendosomal transport
Biological Processhemopoiesis
Biological Processintracellular iron ion homeostasis
Biological Processlearning or memory
Biological Processmembrane bending
Biological Processmulticellular organismal-level iron ion homeostasis
Biological Processnegative regulation of gene expression
Biological Processnegative regulation of protein localization to cell surface
Biological Processnegative regulation of protein localization to plasma membrane
Biological Processnegative regulation of receptor-mediated endocytosis
Biological Processpositive regulation of amyloid precursor protein catabolic process
Biological Processpositive regulation of amyloid-beta formation
Biological Processpositive regulation of axonogenesis
Biological Processpositive regulation of dendrite extension
Biological Processpositive regulation of DNA-templated transcription
Biological Processpositive regulation of Ras protein signal transduction
Biological Processpositive regulation of synaptic vesicle clustering
Biological Processpositive regulation of synaptic vesicle endocytosis
Biological Processreceptor internalization
Biological Processreceptor-mediated endocytosis
Biological Processregulation of amyloid precursor protein catabolic process
Biological Processregulation of endocytosis
Biological Processregulation of protein localization
Biological Processregulation of protein transport
Biological Processregulation of synaptic vesicle endocytosis
Biological Processregulation of synaptic vesicle transport
Biological Processregulation of terminal button organization
Biological Processregulation of vesicle size
Biological Processsynaptic vesicle budding from presynaptic endocytic zone membrane
Biological Processsynaptic vesicle endocytosis
Biological Processsynaptic vesicle maturation
Biological Processvesicle budding from membrane
Biological Processvesicle cargo loading
Biological Processvesicle-mediated transport

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphatidylinositol-binding clathrin assembly protein
  • Alternative names
    • Clathrin assembly lymphoid myeloid leukemia protein (rCALM)

Gene names

    • Name
      Picalm
    • Synonyms
      Calm

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Sprague-Dawley
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    O55012
  • Secondary accessions
    • O55011

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain, cross-link.

TypeIDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylserine
ChainPRO_00001870642-640Phosphatidylinositol-binding clathrin assembly protein
Modified residue16Phosphoserine
Modified residue20Phosphoserine
Cross-link238Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)
Modified residue303Phosphoserine
Modified residue315Phosphoserine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Isoform 2 was found in most tissues examined. Isoform 1 has an overlapping expression pattern but is absent from lung, heart and pancreas. Both isoforms are widely expressed in the brain, higher levels are seen in hippocampus, dentate gyrus, medial habenula nucleus and cerebellar granule cells.

Interaction

Subunit

Binds to clathrin; involves primarily the C-terminal sequences, but the full-length protein is required for full binding capacity. Binds phosphatidylinositol 4,5- bisphosphate. Interacts with PIMREG; this interaction may change the subcellular location into the nucleus. Interacts with AP2A1 (via its alpha-appendage domain). Interacts (via N-terminus) with VAMP2; VAMP3; VAMP7 and VAMP8 (Via N-terminus). Interacts with LC3/MAP1LC3A.

Binary interactions

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain14-145ENTH
Region221-294Interaction with PIMREG
Region543-568Disordered

Sequence similarities

Belongs to the PICALM/SNAP91 family.

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

O55012-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Name
    1
  • Synonyms
    Long
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Length
    640
  • Mass (Da)
    69,286
  • Last updated
    1998-06-01 v1
  • Checksum
    7395A92C285FA10A
MSGQSLTDRITAAQHSVTGSAVSKTVCKATTHEIMGPKKKHLDYLIQCTNEMNVNIPQLADSLFERTTNSSWVVVFKSLITTHHLMVYGNERFIQYLASRNTLFNLSNFLDKSGLQGYDMSTFIRRYSRYLNEKAVSYRQVAFDFTKVKRGADGVMRTMNTEKLLKTVPIIQNQMDALLDFNVNSNELTNGVINAAFMLLFKDAIRLFAAYNEGIINLLEKYFDMKKNQCKEGLDIYKKFLTRMTRISEFLKVAEQVGIDRGDIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRLKELAKKPHTSLTTAASPVSTSAGGIMTAPAIDIFSTPSSSNSTSKLPNDLLDLQQPTFHPSVHAMSAAPQVASTWGDAVDDAIPSLNPFLTKSSGDVHLPISSDVSTFTTRTPTHEMFVGFSPSPVTQPHPSAGLNVDFESVFGNKSTNVAVDSGGGLLKPTVASQNQSLPVAKLPPNKLVSDDLDSSLANLVGNLGIGNGTTKNDVSCSQPGEKKLTGGSNWQPKVAPTTAWSAATMAPPVMAYPATTPTGMIGYGIPPQMGSVPVMTQPTLIYSQPVMRPPNPFGPVPGAQIQFM

O55012-2

  • Name
    2
  • Synonyms
    Short
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There are 4 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A0G2JTT2A0A0G2JTT2_RATPicalm660
A0A0G2JUQ5A0A0G2JUQ5_RATPicalm81
A0A1B0GWY4A0A1B0GWY4_RATPicalm652
A0A8I6G718A0A8I6G718_RATPicalm550

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_050687420-462in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF041373
EMBL· GenBank· DDBJ
AAB97078.1
EMBL· GenBank· DDBJ
mRNA
AF041374
EMBL· GenBank· DDBJ
AAB97079.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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