O54909 · RDH16_MOUSE
- ProteinRetinol dehydrogenase 16
- GeneRdh16
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids317 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Oxidoreductase with a preference for NAD. Oxidizes all-trans-retinol, 9-cis-retinol, 11-cis-retinol and 13-cis-retinol to the corresponding aldehydes. Has higher activity towards CRBP-bound retinol than with free retinol. Oxidizes 3-alpha-hydroxysteroids. Oxidizes androstanediol and androsterone to dihydrotestosterone and androstanedione. Can also catalyze the reverse reaction.
Catalytic activity
- all-trans-retinol--[retinol-binding protein] + NAD+ = all-trans-retinal--[retinol-binding protein] + H+ + NADH
- 9-cis-retinol + NAD+ = 9-cis-retinal + H+ + NADH
- 13-cis-retinol + NAD+ = 13-cis-retinal + H+ + NADH
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.2 μM | 3alpha-hydroxy-5alpha-androstan-17-one (androsterone) |
Pathway
Cofactor metabolism; retinol metabolism.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum lumen | |
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | intracellular membrane-bounded organelle | |
Cellular Component | organelle membrane | |
Molecular Function | 11-cis-retinol dehydrogenase | |
Molecular Function | all-trans-retinol dehydrogenase (NAD+) activity | |
Molecular Function | androstan-3-alpha,17-beta-diol dehydrogenase activity | |
Molecular Function | androsterone dehydrogenase activity | |
Molecular Function | identical protein binding | |
Biological Process | 9-cis-retinoic acid biosynthetic process | |
Biological Process | positive regulation of retinoic acid biosynthetic process | |
Biological Process | retinoic acid metabolic process | |
Biological Process | retinoid metabolic process | |
Biological Process | retinol metabolic process | |
Biological Process | steroid metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameRetinol dehydrogenase 16
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionO54909
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 289-309 | Helical | ||||
Sequence: LFYLPLSYLPTFLVDALLYWT |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000446674 | 1-317 | Retinol dehydrogenase 16 | |||
Sequence: MWLYLVALVGLWTLLRFFRVRQVVSHLQDKYVFITGCDSGFGTLLARQLDRRGMRVLAACLTEKGAEELRNKTSDRLETVILDVTKTESIVTATQWVKEHVGNRGLWGLVNNAGISTPSGPNEWMKKQDFAHVLDVNLLGMIEVTLSMLPLVRKARGRVVNVSSVMGRVSLFGGGYCISKYGVEAFSDSLRRELSYFGVKVAIIEPGFFLTGVTSSARLCSNTQMLWDQTSSEIREIYGEKYLASYLKRLNKLDKRCNKDLSGVTDCMEHALTACHPRTRYSAGWDAKLFYLPLSYLPTFLVDALLYWTSLKPEKAL |
Post-translational modification
Not glycosylated.
Proteomic databases
PTM databases
Expression
Gene expression databases
Structure
Family & Domains
Domain
The C-terminal region plays a crucial role in controlling the activity of RDH16 and its required for endoplasmic reticulum (ER) retention.
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length317
- Mass (Da)35,744
- Last updated1998-06-01 v1
- ChecksumB3D82F3F1D5D57C8
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF030513 EMBL· GenBank· DDBJ | AAB97166.1 EMBL· GenBank· DDBJ | mRNA | ||
AC131690 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH466578 EMBL· GenBank· DDBJ | EDL24525.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC089612 EMBL· GenBank· DDBJ | AAH89612.1 EMBL· GenBank· DDBJ | mRNA |