O54785 · LIMK2_MOUSE
- ProteinLIM domain kinase 2
- GeneLimk2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids638 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP. Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (By similarity).
Catalytic activity
- ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]This reaction proceeds in the forward direction.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | centrosome | |
Cellular Component | cis-Golgi network | |
Cellular Component | cytoplasm | |
Cellular Component | mitotic spindle | |
Cellular Component | nucleus | |
Cellular Component | perinuclear region of cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | protein kinase activity | |
Molecular Function | protein serine kinase activity | |
Molecular Function | protein serine/threonine kinase activity | |
Biological Process | actin cytoskeleton organization | |
Biological Process | astral microtubule organization | |
Biological Process | cornea development in camera-type eye | |
Biological Process | establishment of vesicle localization | |
Biological Process | head development | |
Biological Process | negative regulation of cilium assembly | |
Biological Process | positive regulation of protein localization to nucleus | |
Biological Process | positive regulation of protein phosphorylation | |
Biological Process | protein phosphorylation | |
Biological Process | spermatogenesis |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLIM domain kinase 2
- EC number
- Short namesLIMK-2
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionO54785
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000075810 | 1-638 | LIM domain kinase 2 | |||
Sequence: MAALAGDEAWRCRGCGTYVPLSQRLYRTANEAWHGSCFRCSECQESLTNWYYEKDGKLYCHKDYWAKFGEFCHGCSLLMTGPAMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQDQLPYSVTLISMPATTECRRGFSVTVESASSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAIKQTSQTLQLLIEHDPVPQRLDQLRLDARLPPHMQSTGHTLMLSTLDTKENQEGTLRRRSLRRSNSISKSPGPSSPKEPLLLSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSVDPFPWQQKVRFAKGISSGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRPPVEKATTKKRTLRKSDRKKRYTVVGNPYWMAPEMLNGKSYDETVDVFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCKLEPESRPAFSKLEDSFEALSLFLGELAIPLPAELEDLDHTVSMEYGLTRDSPP | ||||||
Modified residue | 210 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 293 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 298 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 505 | Phosphothreonine; by ROCK1 and CDC42BP | ||||
Sequence: T |
Post-translational modification
Phosphorylated on serine and/or threonine residues by ROCK1.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 12-63 | LIM zinc-binding 1 | ||||
Sequence: CRGCGTYVPLSQRLYRTANEAWHGSCFRCSECQESLTNWYYEKDGKLYCHKD | ||||||
Domain | 72-124 | LIM zinc-binding 2 | ||||
Sequence: CHGCSLLMTGPAMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKC | ||||||
Domain | 152-239 | PDZ | ||||
Sequence: LISMPATTECRRGFSVTVESASSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAIKQTSQTLQLLIEHDP | ||||||
Region | 255-304 | Disordered | ||||
Sequence: PHMQSTGHTLMLSTLDTKENQEGTLRRRSLRRSNSISKSPGPSSPKEPLL | ||||||
Domain | 331-608 | Protein kinase | ||||
Sequence: LIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSVDPFPWQQKVRFAKGISSGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRPPVEKATTKKRTLRKSDRKKRYTVVGNPYWMAPEMLNGKSYDETVDVFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCKLEPESRPAFSKLEDSFEALSLFL |
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing.
O54785-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameLIMK2a
- Length638
- Mass (Da)72,202
- Last updated1998-08-01 v2
- Checksum54CFA5E7A6A79C39
O54785-2
- NameLIMK2b
- Differences from canonical
- 1-37: MAALAGDEAWRCRGCGTYVPLSQRLYRTANEAWHGSC → MGSYLSVPAYFTSRDP
O54785-3
- Name3
- SynonymsLIMK2t, tLIMK2
- Differences from canonical
- 1-187: Missing
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
Q5NC03 | Q5NC03_MOUSE | Limk2 | 623 |
Features
Showing features for alternative sequence.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB008117 EMBL· GenBank· DDBJ | BAA29035.1 EMBL· GenBank· DDBJ | mRNA | ||
AB005140 EMBL· GenBank· DDBJ | BAA24491.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB005131 EMBL· GenBank· DDBJ | BAA24488.1 EMBL· GenBank· DDBJ | mRNA | ||
AB005132 EMBL· GenBank· DDBJ | BAA24489.1 EMBL· GenBank· DDBJ | mRNA | ||
AB005134 EMBL· GenBank· DDBJ | BAA24490.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U88618 EMBL· GenBank· DDBJ | AAC39947.1 EMBL· GenBank· DDBJ | mRNA | ||
AB012291 EMBL· GenBank· DDBJ | BAA32437.1 EMBL· GenBank· DDBJ | mRNA | ||
AB012092 EMBL· GenBank· DDBJ | BAA31147.1 EMBL· GenBank· DDBJ | mRNA | ||
BC007129 EMBL· GenBank· DDBJ | AAH07129.1 EMBL· GenBank· DDBJ | mRNA |