O54329 · LANA_STRMG

  • Protein
    Lantibiotic mutacin-2
  • Gene
    mutA
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria including M.luteus, S.aureus, Streptococcus, P.micros, P.acidilactici, C.sporogenes, C.diphtheriae, A.viscosus, G.vaginalis, P.acnes, L.monocytogenes and M.smegmatis, and Gram-negative bacteria including C.jejuni, H.pylori and N.gonorrhoeae. Transiently and partially depolarizes the transmembrane electrical potential and pH gradient of susceptible cells, inhibits the uptake of amino acids and depletes the intracellular ATP pool.

pH Dependence

Stable from pH 2.0 to 4.0. Activity decreases gradually with increasing pH.

Temperature Dependence

Thermostable.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Molecular Functionsignaling receptor binding
Biological Processamino acid transport
Biological Processdefense response to Gram-negative bacterium
Biological Processdefense response to Gram-positive bacterium
Biological Processkilling of cells of another organism
Biological Processregulation of membrane potential

Keywords

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Lantibiotic mutacin-2
  • Alternative names
    • Lantibiotic mutacin H-29B
    • Mutacin II

Gene names

    • Name
      mutA

Organism names

  • Taxonomic identifier
  • Strains
    • T8
    • 29B
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Lactobacillales > Streptococcaceae > Streptococcus

Accessions

  • Primary accession
    O54329
  • Secondary accessions
    • P84110

Subcellular Location

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis14No loss of activity or protein production.
Mutagenesis14Reduced protein production to about 10% of wild-type level.
Mutagenesis15No loss of activity or protein production.
Mutagenesis15Reduced protein production to about 50% of wild-type level.
Mutagenesis16No loss of activity or protein production.
Mutagenesis19No loss of activity or protein production.
Mutagenesis19Reduced protein production to about 75% of wild-type level.
Mutagenesis20No mature protein is produced.
Mutagenesis20No loss of activity or protein production.
Mutagenesis23No mature protein is produced.
Mutagenesis23No loss of activity or protein production.
Mutagenesis25No mature protein is produced.
Mutagenesis26No mature protein is produced.
Mutagenesis27No protein is secreted.
Mutagenesis33No loss of activity.
Mutagenesis35Low activity, less than 10% of wild-type.
Mutagenesis36Loss of secretion.
Mutagenesis36No loss of activity.
Mutagenesis41Loss of activity.
Mutagenesis52Loss of activity.
Mutagenesis53Low activity, less than 10% of wild-type level.

PTM/Processing

Features

Showing features for propeptide, peptide, cross-link, modified residue.

TypeIDPosition(s)Description
PropeptidePRO_00000171281-26
PeptidePRO_000001712927-53Lantibiotic mutacin-2
Cross-link36↔41Beta-methyllanthionine (Thr-Cys)
Cross-link38↔52Lanthionine (Ser-Cys)
Cross-link45↔53Lanthionine (Ser-Cys)
Modified residue512,3-didehydrobutyrine

Post-translational modification

Maturation of lantibiotics involves the enzymatic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.
It is not established whether the 2,3-didehydrobutyrine is the E- or Z-isomer (PubMed:10821848, PubMed:16626493, PubMed:8021218, PubMed:8660519, PubMed:9647795).

Keywords

Family & Domains

Sequence similarities

Belongs to the type A lantibiotic family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    53
  • Mass (Da)
    6,020
  • Last updated
    1998-06-01 v1
  • Checksum
    6C3788E2C9EC6525
MNKLNSNAVVSLNEVSDSELDTILGGNRWWQGVVPTVSYECRMNSWQHVFTCC

Mass Spectrometry

Molecular mass is 3,244.64 Da. Determined by Electrospray.
Molecular mass is 3,245.4 Da. Determined by Electrospray.
Molecular mass is 3,246.08 Da. Determined by MALDI.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U40620
EMBL· GenBank· DDBJ
AAC38144.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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