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O54288 · KDGA_SACSO

Function

function

Involved in the degradation of glucose and galactose via the Entner-Doudoroff pathway. Catalyzes the reversible cleavage of 2-keto-3-deoxy-6-phosphogluconate (KDPG) and 2-keto-3-deoxygluconate (KDG) forming pyruvate and glyceraldehyde 3-phosphate or glyceraldehyde, respectively. It is also able to catalyze the reversible cleavage of 2-keto-3-deoxy-6-phosphogalactonate (KDPGal) and 2-keto-3-deoxygalactonate (KDGal). It is equally active with both D- and L-glyceraldehyde.

Miscellaneous

The metabolic pathway in Sulfolobus species contrasts with the situation observed in other microorganisms where separate enzymes exist for the catabolism of the two sugars, glucose and galactose.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.1 mMKDPG660
0.17 mMKDPGal660
3.9 mMpyruvate670
3.9 mMD-glyceraldehyde670
5.2 mMD,L-glyceraldehyde670
7.1 mML-glyceraldehyde670
9.9 mMKDGal660
25.7 mMKDG660
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
12.3 μmol/min/mg660with KDGal as substrate
15.7 μmol/min/mg670with pyruvate as substrate
17.1 μmol/min/mg670with D,L-glyceraldehyde as substrate
18 μmol/min/mg670with D-glyceraldehyde as substrate
18 μmol/min/mg670with L-glyceraldehyde as substrate
51.4 μmol/min/mg660with KDG as substrate
kcat is 28.2 sec-1 for KDG and 6.8 sec-1 for KDGal.

Temperature Dependence

Extremely thermostable.

Pathway

Carbohydrate acid metabolism; 2-dehydro-3-deoxy-D-gluconate degradation; D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate: step 2/2.

Features

Showing features for binding site, site, active site.

Type
IDPosition(s)Description
Binding site43-44substrate
Site130Proton shuttle
Binding site130-132substrate
Active site155Schiff-base intermediate with substrate
Binding site155-157substrate

GO annotations

AspectTerm
Molecular Function2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
Molecular Function2-dehydro-3-deoxy-phosphogluconate aldolase activity
Molecular Function4-hydroxy-tetrahydrodipicolinate synthase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase
  • EC number

Organism names

Accessions

  • Primary accession
    O54288

PTM/Processing

Features

Showing features for initiator methionine, chain.

Type
IDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00004226582-2942-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase

Interaction

Subunit

Homotetramer; dimer of dimers.

Family & Domains

Sequence similarities

Belongs to the DapA family. KDPG aldolase subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    294
  • Mass (Da)
    33,108
  • Last updated
    1998-06-01 v1
  • MD5 Checksum
    B2CB04351F9B4C303E519B4ACE4B4A9F
MPEIITPIITPFTKDNRIDKEKLKIHAENLIRKGIDKLFVNGTTGLGPSLSPEEKLENLKAVYDVTNKIIFQVGGLNLDDAIRLAKLSKDFDIVGIASYAPYYYPRMSEKHLVKYFKTLCEVSPHPVYLYNYPTATGKDIDAKVAKEIGCFTGVKDTIENIIHTLDYKRLNPNMLVYSGSDMLIATVASTGLDGNVAAGSNYLPEVTVTIKKLAMERKIDEALKLQFLHDEVIEASRIFGSLSSNYVLTKYFQGYDLGYPRPPIFPLDDEEERQLIKKVEGIRAKLVELKILKE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AJ224174
EMBL· GenBank· DDBJ
CAA11866.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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