O54288 · KDGA_SACSO
- Protein2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids294 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the degradation of glucose and galactose via the Entner-Doudoroff pathway. Catalyzes the reversible cleavage of 2-keto-3-deoxy-6-phosphogluconate (KDPG) and 2-keto-3-deoxygluconate (KDG) forming pyruvate and glyceraldehyde 3-phosphate or glyceraldehyde, respectively. It is also able to catalyze the reversible cleavage of 2-keto-3-deoxy-6-phosphogalactonate (KDPGal) and 2-keto-3-deoxygalactonate (KDGal). It is equally active with both D- and L-glyceraldehyde.
Miscellaneous
The metabolic pathway in Sulfolobus species contrasts with the situation observed in other microorganisms where separate enzymes exist for the catabolism of the two sugars, glucose and galactose.
Catalytic activity
- 2-dehydro-3-deoxy-6-phospho-D-gluconate = D-glyceraldehyde 3-phosphate + pyruvate
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.1 mM | KDPG | 6 | 60 | |||
0.17 mM | KDPGal | 6 | 60 | |||
3.9 mM | pyruvate | 6 | 70 | |||
3.9 mM | D-glyceraldehyde | 6 | 70 | |||
5.2 mM | D,L-glyceraldehyde | 6 | 70 | |||
7.1 mM | L-glyceraldehyde | 6 | 70 | |||
9.9 mM | KDGal | 6 | 60 | |||
25.7 mM | KDG | 6 | 60 |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
12.3 μmol/min/mg | 6 | 60 | with KDGal as substrate | ||
15.7 μmol/min/mg | 6 | 70 | with pyruvate as substrate | ||
17.1 μmol/min/mg | 6 | 70 | with D,L-glyceraldehyde as substrate | ||
18 μmol/min/mg | 6 | 70 | with D-glyceraldehyde as substrate | ||
18 μmol/min/mg | 6 | 70 | with L-glyceraldehyde as substrate | ||
51.4 μmol/min/mg | 6 | 60 | with KDG as substrate |
kcat is 28.2 sec-1 for KDG and 6.8 sec-1 for KDGal.
Temperature Dependence
Extremely thermostable.
Pathway
Carbohydrate acid metabolism; 2-dehydro-3-deoxy-D-gluconate degradation; D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate: step 2/2.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 43-44 | substrate | |||
Site | 130 | Proton shuttle | |||
Binding site | 130-132 | substrate | |||
Active site | 155 | Schiff-base intermediate with substrate | |||
Binding site | 155-157 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity | |
Molecular Function | 2-dehydro-3-deoxy-phosphogluconate aldolase activity | |
Molecular Function | 4-hydroxy-tetrahydrodipicolinate synthase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase
- EC number
Organism names
- Strain
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Sulfolobales > Sulfolobaceae > Saccharolobus
Accessions
- Primary accessionO54288
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Initiator methionine | 1 | Removed | |||
Chain | PRO_0000422658 | 2-294 | 2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase | ||
Interaction
Subunit
Homotetramer; dimer of dimers.
Structure
Sequence
- Sequence statusComplete
- Length294
- Mass (Da)33,108
- Last updated1998-06-01 v1
- MD5 ChecksumB2CB04351F9B4C303E519B4ACE4B4A9F
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ224174 EMBL· GenBank· DDBJ | CAA11866.1 EMBL· GenBank· DDBJ | Genomic DNA |