O53585 · GLFT2_MYCTU

Function

function

Involved in the galactan polymerization of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacteria cell wall. Thus, successively transfers approximately 28 galactofuranosyl (Galf) residues from UDP-galactofuranose (UDP-Galf) onto the galactofuranosyl-galactofuranosyl-rhamnosyl-GlcNAc-diphospho-decaprenol (Galf-Galf-Rha-GlcNAc-PP-C50) acceptor produced by GlfT1, with alternating 1->5 and 1->6 links, forming a galactan domain with approximately 30 galactofuranosyl residues.

Miscellaneous

Was identified as a high-confidence drug target.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.38 mMUDP-Galf
0.6 mMbeta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-octyl
1.7 mMbeta-D-Galf-(1->5)-beta-D-Galf-octyl
0.635 mMbeta-D-Galf-(1->6)-beta-D-Galf-octyl
0.208 mMbeta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-octyl
0.204 mMbeta-D-Galf-(1->6)-beta-D-Galf-(1->5)-beta-D-Galf-octyl
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
4.4 μmol/min/mgfor galtactose transfer on beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-octyl
kcat is 430 min-1 for galtactose transfer on beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-octyl.

Pathway

Cell wall biogenesis; cell wall polysaccharide biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site171UDP-alpha-D-galactofuranose (UniProtKB | ChEBI)
Binding site200UDP-alpha-D-galactofuranose (UniProtKB | ChEBI)
Binding site229UDP-alpha-D-galactofuranose (UniProtKB | ChEBI)
Binding site256Mn2+ (UniProtKB | ChEBI)
Binding site256UDP-alpha-D-galactofuranose (UniProtKB | ChEBI)
Binding site258Mn2+ (UniProtKB | ChEBI)
Active site372Proton acceptor
Binding site396Mn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentplasma membrane
Molecular Functionglycosyltransferase activity
Molecular Functionlipopolysaccharide-1,5-galactosyltransferase activity
Molecular Functionlipopolysaccharide-1,6-galactosyltransferase activity
Molecular Functionmetal ion binding
Molecular Functiontransferase activity
Molecular FunctionUDP-galactosyltransferase activity
Biological Processcapsule polysaccharide biosynthetic process
Biological Processcell wall macromolecule biosynthetic process
Biological Processcell wall organization
Biological Processcell wall polysaccharide biosynthetic process
Biological Processlipopolysaccharide biosynthetic process
Biological Processmycolate cell wall layer assembly
Biological ProcessUDP-D-galactose metabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

    • GT2Glycosyltransferase Family 2

Names & Taxonomy

Protein names

  • Recommended name
    Galactofuranosyltransferase GlfT2
  • EC number
  • Short names
    GalTr 2
  • Alternative names
    • Arabinogalactan galactosyltransferase 2
    • Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase
    • Polymerizing galactofuranosyltransferase GlfT2

Gene names

    • Name
      glfT2
    • Synonyms
      glfT
    • Ordered locus names
      Rv3808c

Organism names

Accessions

  • Primary accession
    O53585
  • Secondary accessions
    • L0TFB5

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis3001400-fold decrease in transferase activity. No effect on affinity for the substrate UDP-Galf.
Mutagenesis371Loss of transferase activity.
Mutagenesis372Loss of transferase activity.
Mutagenesis3991200-fold decrease in transferase activity. No effect on affinity for the substrate UDP-Galf.
Mutagenesis4131700-fold decrease in transferase activity. No effect on affinity for the substrate UDP-Galf.

Chemistry

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003953561-637Galactofuranosyltransferase GlfT2

Proteomic databases

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Chemistry

Family & Domains

Sequence similarities

Belongs to the glycosyltransferase 2 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    637
  • Mass (Da)
    71,507
  • Last updated
    1998-06-01 v1
  • Checksum
    D7C9AB28B5005B66
MSELAASLLSRVILPRPGEPLDVRKLYLEESTTNARRAHAPTRTSLQIGAESEVSFATYFNAFPASYWRRWTTCKSVVLRVQVTGAGRVDVYRTKATGARIFVEGHDFTGTEDQPAAVETEVVLQPFEDGGWVWFDITTDTAVTLHSGGWYATSPAPGTANIAVGIPTFNRPADCVNALRELTADPLVDQVIGAVIVPDQGERKVRDHPDFPAAAARLGSRLSIHDQPNLGGSGGYSRVMYEALKNTDCQQILFMDDDIRLEPDSILRVLAMHRFAKAPMLVGGQMLNLQEPSHLHIMGEVVDRSIFMWTAAPHAEYDHDFAEYPLNDNNSRSKLLHRRIDVDYNGWWTCMIPRQVAEELGQPLPLFIKWDDADYGLRAAEHGYPTVTLPGAAIWHMAWSDKDDAIDWQAYFHLRNRLVVAAMHWDGPKAQVIGLVRSHLKATLKHLACLEYSTVAIQNKAIDDFLAGPEHIFSILESALPQVHRIRKSYPDAVVLPAASELPPPLHKNKAMKPPVNPLVIGYRLARGIMHNLTAANPQHHRRPEFNVPTQDARWFLLCTVDGATVTTADGCGVVYRQRDRAKMFALLWQSLRRQRQLLKRFEEMRRIYRDALPTLSSKQKWETALLPAANQEPEHG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL123456
EMBL· GenBank· DDBJ
CCP46637.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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