O53585 · GLFT2_MYCTU
- ProteinGalactofuranosyltransferase GlfT2
- GeneglfT2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids637 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the galactan polymerization of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacteria cell wall. Thus, successively transfers approximately 28 galactofuranosyl (Galf) residues from UDP-galactofuranose (UDP-Galf) onto the galactofuranosyl-galactofuranosyl-rhamnosyl-GlcNAc-diphospho-decaprenol (Galf-Galf-Rha-GlcNAc-PP-C50) acceptor produced by GlfT1, with alternating 1->5 and 1->6 links, forming a galactan domain with approximately 30 galactofuranosyl residues.
Miscellaneous
Was identified as a high-confidence drug target.
Catalytic activity
- beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octa-cis-decaprenol + 28 UDP-alpha-D-galactofuranose = [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octa-cis-decaprenol + 28 H+ + 28 UDP
Cofactor
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.38 mM | UDP-Galf | |||||
0.6 mM | beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-octyl | |||||
1.7 mM | beta-D-Galf-(1->5)-beta-D-Galf-octyl | |||||
0.635 mM | beta-D-Galf-(1->6)-beta-D-Galf-octyl | |||||
0.208 mM | beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-octyl | |||||
0.204 mM | beta-D-Galf-(1->6)-beta-D-Galf-(1->5)-beta-D-Galf-octyl |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
4.4 μmol/min/mg | for galtactose transfer on beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-octyl |
kcat is 430 min-1 for galtactose transfer on beta-D-Galf-(1->5)-beta-D-Galf-(1->6)-beta-D-Galf-octyl.
Pathway
Cell wall biogenesis; cell wall polysaccharide biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 171 | UDP-alpha-D-galactofuranose (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 200 | UDP-alpha-D-galactofuranose (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 229 | UDP-alpha-D-galactofuranose (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 256 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 256 | UDP-alpha-D-galactofuranose (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 258 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 372 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 396 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | plasma membrane | |
Molecular Function | glycosyltransferase activity | |
Molecular Function | lipopolysaccharide-1,5-galactosyltransferase activity | |
Molecular Function | lipopolysaccharide-1,6-galactosyltransferase activity | |
Molecular Function | metal ion binding | |
Molecular Function | transferase activity | |
Molecular Function | UDP-galactosyltransferase activity | |
Biological Process | capsule polysaccharide biosynthetic process | |
Biological Process | cell wall macromolecule biosynthetic process | |
Biological Process | cell wall organization | |
Biological Process | cell wall polysaccharide biosynthetic process | |
Biological Process | lipopolysaccharide biosynthetic process | |
Biological Process | mycolate cell wall layer assembly | |
Biological Process | UDP-D-galactose metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameGalactofuranosyltransferase GlfT2
- EC number
- Short namesGalTr 2
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis complex
Accessions
- Primary accessionO53585
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 300 | 1400-fold decrease in transferase activity. No effect on affinity for the substrate UDP-Galf. | ||||
Sequence: E → S | ||||||
Mutagenesis | 371 | Loss of transferase activity. | ||||
Sequence: D → S | ||||||
Mutagenesis | 372 | Loss of transferase activity. | ||||
Sequence: D → S | ||||||
Mutagenesis | 399 | 1200-fold decrease in transferase activity. No effect on affinity for the substrate UDP-Galf. | ||||
Sequence: W → S | ||||||
Mutagenesis | 413 | 1700-fold decrease in transferase activity. No effect on affinity for the substrate UDP-Galf. | ||||
Sequence: H → S |
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000395356 | 1-637 | Galactofuranosyltransferase GlfT2 | |||
Sequence: MSELAASLLSRVILPRPGEPLDVRKLYLEESTTNARRAHAPTRTSLQIGAESEVSFATYFNAFPASYWRRWTTCKSVVLRVQVTGAGRVDVYRTKATGARIFVEGHDFTGTEDQPAAVETEVVLQPFEDGGWVWFDITTDTAVTLHSGGWYATSPAPGTANIAVGIPTFNRPADCVNALRELTADPLVDQVIGAVIVPDQGERKVRDHPDFPAAAARLGSRLSIHDQPNLGGSGGYSRVMYEALKNTDCQQILFMDDDIRLEPDSILRVLAMHRFAKAPMLVGGQMLNLQEPSHLHIMGEVVDRSIFMWTAAPHAEYDHDFAEYPLNDNNSRSKLLHRRIDVDYNGWWTCMIPRQVAEELGQPLPLFIKWDDADYGLRAAEHGYPTVTLPGAAIWHMAWSDKDDAIDWQAYFHLRNRLVVAAMHWDGPKAQVIGLVRSHLKATLKHLACLEYSTVAIQNKAIDDFLAGPEHIFSILESALPQVHRIRKSYPDAVVLPAASELPPPLHKNKAMKPPVNPLVIGYRLARGIMHNLTAANPQHHRRPEFNVPTQDARWFLLCTVDGATVTTADGCGVVYRQRDRAKMFALLWQSLRRQRQLLKRFEEMRRIYRDALPTLSSKQKWETALLPAANQEPEHG |
Proteomic databases
Structure
Sequence
- Sequence statusComplete
- Length637
- Mass (Da)71,507
- Last updated1998-06-01 v1
- ChecksumD7C9AB28B5005B66
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL123456 EMBL· GenBank· DDBJ | CCP46637.1 EMBL· GenBank· DDBJ | Genomic DNA |