O53168 · RIPA_MYCTU

Function

function

Peptidoglycan endopeptidase that cleaves the bond between D-glutamate and meso-diaminopimelate. Binds and degrades high-molecular weight peptidoglycan from a number of Actinobacteria; activity is increased in the presence of RpfB and inhibited by PBP1A (ponA1). Required for normal separation of daughter cells after cell division and for cell wall integrity. Required for host cell invasion and intracellular survival in host macrophages.

Miscellaneous

Was identified as a high-confidence drug target.

Caution

The role of the N-terminal domain is controversial. A recent paper (PubMed:21864539) found it had no effect on enzyme activity, while another (PubMed:20826344) reported that it had an inhibitory role and had to be cleaved off for full enzyme activity.

Activity regulation

MoxR1-mediated folding is critical for secretion via the TAT system (PubMed:26933057).
The synergistic effects on peptidoglycan degradation of RipA plus RpfB are inhibited by addition of PBP1A (ponA1) (PubMed:20686708).

pH Dependence

Optimum pH is between 5 and 5.5.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site383Nucleophile
Active site432Proton acceptor
Active site444

GO annotations

AspectTerm
Cellular Componentextracellular region
Cellular Componentpeptidoglycan-based cell wall
Molecular Functioncysteine-type peptidase activity
Molecular FunctionN-acetylmuramoyl-L-alanine amidase activity
Biological Processcell wall organization
Biological Processcell wall organization or biogenesis
Biological Processproteolysis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Peptidoglycan endopeptidase RipA
  • EC number
  • Alternative names
    • Macrophage invasion and intracellular persistence protein A
    • Resuscitation-promoting factor interaction partner A (Rpf-interacting protein A)

Gene names

    • Name
      ripA
    • Ordered locus names
      Rv1477

Organism names

Accessions

  • Primary accession
    O53168
  • Secondary accessions
    • L0T6Z1

Proteomes

Organism-specific databases

Subcellular Location

Secreted
Note: Secreted by the TAT secretion pathway (PubMed:26933057).
Localizes to the septa upon expression in M.bovis or M.smegmatis. Remains associated with the cell

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis382-385Abolishes host cell invasion and survival in host macrophages.
Mutagenesis383Loss of enzyme activity.
Mutagenesis419-421Abolishes host cell invasion.

Miscellaneous

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-39Tat-type signal
ChainPRO_000041376240-472Peptidoglycan endopeptidase RipA

Post-translational modification

Exported by the Tat system (PubMed:26933057).
The position of the signal peptide cleavage has not been experimentally proven (PubMed:26933057).

Proteomic databases

Interaction

Subunit

Monomer. Interacts with RpfB and PBP1A (ponA1) via residues 448-472 of RipA, interacts with RpfE (PubMed:17919286, PubMed:20686708, PubMed:20826344, PubMed:21864539).
Interacts with the chaperone MoxR1 (PubMed:26933057).
RipA-MoxR1 interaction in the cytoplasm leads to proper folding of RipA, resulting in its secretion (PubMed:26933057).
Also interacts with Mce2B (PubMed:26933057).

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain340-472NlpC/P60

Sequence similarities

Belongs to the peptidase C40 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    472
  • Mass (Da)
    49,808
  • Last updated
    1998-06-01 v1
  • Checksum
    3B7D83D29E3631DD
MRRNRRGSPARPAARFVRPAIPSALSVALLVCTPGLATADPQTDTIAALIADVAKANQRLQDLSDEVQAEQESVNKAMVDVETARDNAAAAEDDLEVSQRAVKDANAAIAAAQHRFDTFAAATYMNGPSVSYLSASSPDEIIATVTAAKTLSASSQAVMANLQRARTERVNTESAARLAKQKADKAAADAKASQDAAVAALTETRRKFDEQREEVQRLAAERDAAQARLQAARLVAWSSEGGQGAPPFRMWDPGSGPAGGRAWDGLWDPTLPMIPSANIPGDPIAVVNQVLGISATSAQVTANMGRKFLEQLGILQPTDTGITNAPAGSAQGRIPRVYGRQASEYVIRRGMSQIGVPYSWGGGNAAGPSKGIDSGAGTVGFDCSGLVLYSFAGVGIKLPHYSGSQYNLGRKIPSSQMRRGDVIFYGPNGSQHVTIYLGNGQMLEAPDVGLKVRVAPVRTAGMTPYVVRYIEY

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL123456
EMBL· GenBank· DDBJ
CCP44237.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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