O50235 · CARA_ZYMMO

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site41L-glutamine (UniProtKB | ChEBI)
Binding site235L-glutamine (UniProtKB | ChEBI)
Binding site237L-glutamine (UniProtKB | ChEBI)
Active site264Nucleophile
Binding site265L-glutamine (UniProtKB | ChEBI)
Binding site268L-glutamine (UniProtKB | ChEBI)
Binding site306L-glutamine (UniProtKB | ChEBI)
Binding site308L-glutamine (UniProtKB | ChEBI)
Binding site309L-glutamine (UniProtKB | ChEBI)
Active site348
Active site350

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • Ordered locus names
      ZMO1618

Organism names

Accessions

  • Primary accession
    O50235
  • Secondary accessions
    • Q5NM18

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001123541-374Carbamoyl phosphate synthase small chain

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-183CPSase
Domain187-374Glutamine amidotransferase type-1

Sequence similarities

Belongs to the CarA family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    374
  • Mass (Da)
    40,808
  • Last updated
    2002-07-11 v2
  • Checksum
    4C6BFA63818FE09E
MVLADGQMIWGRGFGATGAKVGEVCFHTAMTGYEEIMTDPSFASQIINFTFPHIGNVGANREDVEAKTLHALGCIVREDVTAPSNFRSLWRFDSWLKDHDRIGLAGVDTRALTGLLRKKGAQNAVIAHDPKGQFDIPALIETARSWAGLQGMDLARSVSTHQSYNWQEGIWSLQNGYSVVDNQKGPHVVAIDYGLKHNILRNLVEAGARVTVVKATASFDEVMAHKPDGVFLSNGPGDPAATAEYAVPVIRQLLDIKMPIFGICLGHQLLALAVGATTYKMHQGHRGANHPVKRLDDSKVEITSMNHGFAVATDSLPEQARPTHLSLFDGSLAGLELTDRPAFSVQYHPEASPGPQDSYYLFTKFIDLIAKERP

Sequence caution

The sequence AAC70355.1 differs from that shown. Reason: Erroneous initiation
The sequence AAV90242.1 differs from that shown. Reason: Erroneous initiation

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF086791
EMBL· GenBank· DDBJ
AAC70355.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
AE008692
EMBL· GenBank· DDBJ
AAV90242.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation

Genome annotation databases

Similar Proteins

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