O50146 · LYSY_THET2
- Protein[LysW]-L-2-aminoadipate 6-phosphate reductase
- GenelysY
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids344 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the NADPH-dependent reduction of [LysW]-aminoadipate 6-phosphate to yield [LysW]-aminoadipate 6-semialdehyde.
Catalytic activity
- [amino-group carrier protein]-C-terminal-N-(1-carboxy-5-oxopentan-1-yl)-L-glutamine + NADP+ + phosphate = [amino-group carrier protein]-C-terminal-N-(1-carboxy-5-phosphooxy-5-oxopentan-1-yl)-L-glutamine + H+ + NADPH
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
9.8 μM | [LysW]-aminoadipate 6-semialdehyde | |||||
14 μM | NADP+ | |||||
12000 μM | phosphate |
kcat is 0.96 sec-1.
Pathway
Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 3/5.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 12-15 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: SGYA | ||||||
Binding site | 36-38 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: SRR | ||||||
Binding site | 75 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Active site | 148 | |||||
Sequence: C | ||||||
Binding site | 180 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 184 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 312 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | N-acetyl-gamma-aminoadipyl-phosphate reductase activity | |
Molecular Function | N-acetyl-gamma-glutamyl-phosphate reductase activity | |
Molecular Function | NAD binding | |
Molecular Function | NADP+ binding | |
Biological Process | arginine biosynthetic process | |
Biological Process | lysine biosynthetic process via aminoadipic acid |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name[LysW]-L-2-aminoadipate 6-phosphate reductase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Deinococcota > Deinococci > Thermales > Thermaceae > Thermus
Accessions
- Primary accessionO50146
- Secondary accessions
Proteomes
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 102 | Strong decrease in activity. | ||||
Sequence: R → A | ||||||
Mutagenesis | 148 | Lack of activity. | ||||
Sequence: C → A | ||||||
Mutagenesis | 195 | 5-fold decrease in kcat and 40-fold increase in Km for [LysW]-aminoadipate 6-semialdehyde. | ||||
Sequence: R → A | ||||||
Mutagenesis | 209 | Does not affect activity under basic conditions. Strong decrease of activity under neutral conditions. | ||||
Sequence: H → A | ||||||
Mutagenesis | 258 | Slight decrease in kcat and 17-fold increase in Km for [LysW]-aminoadipate 6-semialdehyde. | ||||
Sequence: R → A | ||||||
Mutagenesis | 271 | 5-fold decrease in kcat and 70-fold increase in Km for [LysW]-aminoadipate 6-semialdehyde. | ||||
Sequence: K → A | ||||||
Mutagenesis | 278 | Lack of activity. | ||||
Sequence: R → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000112501 | 1-344 | [LysW]-L-2-aminoadipate 6-phosphate reductase | |||
Sequence: MDKKTLSIVGASGYAGGEFLRLALSHPYLEVKQVTSRRFAGEPVHFVHPNLRGRTNLKFIPPEKLEPADILVLALPHGVFAREFDRYSALAPILIDLSADFRLKDPELYRRYYGEHPRPDLLGCFVYAVPELYREALKGADWIAGAGCNATATLLGLYPLLKAGVLKPTPIFVTLLISTSAAGAEASPASHHPERAGSIRVYKPTGHRHTAEVVENLPGRPEVHLTAIATDRVRGILMTAQCFVQDGWSERDVWQAYREAYAGEPFIRLVKQKKGVHRYPDPRFVQGTNYADIGFELEEDTGRLVVMTAIDNLVKGTAGHALQALNVRMGWPETLGLDFPGLHP |
Structure
Sequence
- Sequence statusComplete
- Length344
- Mass (Da)38,051
- Last updated2000-12-01 v2
- ChecksumB11CE66CB2F04C15
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE017221 EMBL· GenBank· DDBJ | AAS81884.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AB017109 EMBL· GenBank· DDBJ | BAA74767.1 EMBL· GenBank· DDBJ | mRNA | ||
AB006681 EMBL· GenBank· DDBJ | BAA23878.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation |