O48661 · SPDS2_ARATH
- ProteinSpermidine synthase 2
- GeneSPDSYN2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids340 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
Catalytic activity
- S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine + H+
Pathway
Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 80 | S-adenosyl 3-(methylsulfanyl)propylamine (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 110 | putrescine (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 111 | S-adenosyl 3-(methylsulfanyl)propylamine (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 135 | S-adenosyl 3-(methylsulfanyl)propylamine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 155 | S-adenosyl 3-(methylsulfanyl)propylamine (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 186-187 | S-adenosyl 3-(methylsulfanyl)propylamine (UniProtKB | ChEBI) | ||||
Sequence: DG | ||||||
Active site | 205 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 205 | S-adenosyl 3-(methylsulfanyl)propylamine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 205-208 | putrescine (UniProtKB | ChEBI) | ||||
Sequence: DSSD | ||||||
Binding site | 274 | putrescine (UniProtKB | ChEBI) | ||||
Sequence: Y |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | mitochondrion | |
Cellular Component | plasma membrane | |
Molecular Function | spermidine synthase activity | |
Biological Process | spermidine biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSpermidine synthase 2
- EC number
- Short namesSPDSY 2
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionO48661
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylserine | ||||
Sequence: S | ||||||
Chain | PRO_0000156449 | 2-340 | Spermidine synthase 2 | |||
Sequence: SSTQEASVTDLPVKRPREAEEDNNGGAMETENGGGEIKEPSCMSSIIPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSISNPKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQYFPNVAVGYEDPRVNLIIGDGVAFLKNAAEGTYDAVIVDSSDPIGPAKELFEKPFFESVNRALRPGGVVCTQAESLWLHMDIIEDIVSNCRDIFKGSVNYAWTSVPTYPSGVIGFMLCSSEGPQVDFKKPVSLIDTDESSIKSHCPLKYYNAEIHSAAFCLPSFAKKVIDSKAN |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Heterodimer. Component of a multiprotein complex. Interacts with SPMS and SPDSYN1.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | O48661 | SPDSYN1 Q9ZUB3 | 7 | EBI-1770100, EBI-1770123 | |
BINARY | O48661 | SPMS Q94BN2 | 4 | EBI-1770100, EBI-1770109 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-41 | Disordered | ||||
Sequence: MSSTQEASVTDLPVKRPREAEEDNNGGAMETENGGGEIKEP | ||||||
Domain | 49-286 | PABS | ||||
Sequence: PGWFSEISPMWPGEAHSLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSISNPKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQYFPNVAVGYEDPRVNLIIGDGVAFLKNAAEGTYDAVIVDSSDPIGPAKELFEKPFFESVNRALRPGGVVCTQAESLWLHMDIIEDIVSNCRDIFKGSVNYAWTSVPTYPSGVIGFMLCSS |
Sequence similarities
Belongs to the spermidine/spermine synthase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length340
- Mass (Da)37,140
- Last updated2000-12-01 v2
- Checksum35704CE45ED4128F
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 48-50 | in Ref. 5; BAA24536 | ||||
Sequence: IPG → TRP |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ251297 EMBL· GenBank· DDBJ | CAB61615.1 EMBL· GenBank· DDBJ | mRNA | ||
AC003671 EMBL· GenBank· DDBJ | AAC18808.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE35044.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF375409 EMBL· GenBank· DDBJ | AAK52993.1 EMBL· GenBank· DDBJ | mRNA | ||
AB006693 EMBL· GenBank· DDBJ | BAA24536.1 EMBL· GenBank· DDBJ | mRNA |