O48500 · DUT_BPT5
- ProteinDeoxyuridine 5'-triphosphate nucleotidohydrolase
- GeneDUT
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids148 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
This enzyme decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into viral progeny DNA. This activity is sufficient to exclude uracil from the DNA during phage replication. In the case of dUTPase mutant phages, the host dUTPase activity is not sufficient to exclude uracil from T5 DNA and uracil are incorporated, leading to decreased phage viability.
Catalytic activity
- dUTP + H2O = diphosphate + dUMP + H+
Cofactor
Pathway
Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | dUTP diphosphatase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | dUMP biosynthetic process | |
Biological Process | dUTP catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDeoxyuridine 5'-triphosphate nucleotidohydrolase
- EC number
- Short namesdUTPase
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Demerecviridae > Markadamsvirinae > Tequintavirus > Tequintavirus T5
- Virus hosts
Accessions
- Primary accessionO48500
- Secondary accessions
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000182970 | 1-148 | Deoxyuridine 5'-triphosphate nucleotidohydrolase | |||
Sequence: MIKIKLTHPDCMPKIGSEDAAGMDLRAFFGTNPAADLRAIAPGKSLMIDTGVAVEIPRGWFGLVVPRSSLGKRHLMIANTAGVIDSDYRGTIKMNLYNYGSEMQTLENFERLCQLVVLPHYSTHNFKIVDELEETIRGEGGFGSSGSK |
Expression
Induction
Expressed in the early phase of the viral replicative cycle.
Keywords
- Developmental stage
Sequence
- Sequence statusComplete
- Length148
- Mass (Da)16,204
- Last updated1998-06-01 v1
- Checksum4BD56DB1456EF430
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY543070 EMBL· GenBank· DDBJ | AAS77177.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY692264 EMBL· GenBank· DDBJ | AAU05268.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY587007 EMBL· GenBank· DDBJ | AAX12059.1 EMBL· GenBank· DDBJ | Genomic DNA |