O45717 · NUD2_CAEEL
- ProteinProtein nud-2
- Genenud-2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids293 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Part of a complex with lis-1, which is recruited to the nuclear envelope by unc-83, where, in turn, it recruits dynein to the nuclear surface and regulates nuclear migration in hypodermal precursor cells (PubMed:20005871, PubMed:27697906).
Plays a role in GABAergic synaptic vesicle localization in the ventral nerve cord (PubMed:16996038).
Plays a role in GABAergic synaptic vesicle localization in the ventral nerve cord (PubMed:16996038).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | centrosome | |
Cellular Component | kinesin complex | |
Cellular Component | kinetochore | |
Cellular Component | microtubule associated complex | |
Cellular Component | nuclear envelope | |
Cellular Component | synapse | |
Molecular Function | microtubule binding | |
Biological Process | cell migration | |
Biological Process | centrosome localization | |
Biological Process | chromosome segregation | |
Biological Process | establishment of chromosome localization | |
Biological Process | establishment of mitotic spindle orientation | |
Biological Process | microtubule nucleation | |
Biological Process | mitotic centrosome separation | |
Biological Process | nuclear migration | |
Biological Process | nuclear migration along microfilament | |
Biological Process | synaptic transmission, GABAergic | |
Biological Process | synaptic vesicle transport | |
Biological Process | vesicle transport along microtubule |
Names & Taxonomy
Protein names
- Recommended nameProtein nud-2
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionO45717
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Recruited to the nuclear envelope by unc-83.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
RNAi-mediated knockdown results in viable animals, but a small, but significant number of animals have nuclear migration defects in hyp7 hypodermal precursor cells (PubMed:20005871).
RNAi-mediated knockdown results in an abnormal distribution of GABAergic synaptic vesicles at synaptic termini of the ventral nerve cord (PubMed:16996038).
RNAi-mediated knockdown in combination with exposure to pentylenetetrazole, a GABA antagonist that induces seizures, results in impaired locomotion, stiffened appearance and an increased convulsion incidence as compared to wild-type animals (PubMed:16996038).
RNAi-mediated knockdown results in an abnormal distribution of GABAergic synaptic vesicles at synaptic termini of the ventral nerve cord (PubMed:16996038).
RNAi-mediated knockdown in combination with exposure to pentylenetetrazole, a GABA antagonist that induces seizures, results in impaired locomotion, stiffened appearance and an increased convulsion incidence as compared to wild-type animals (PubMed:16996038).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 239-293 | Abolishes interaction with unc-83 isoform c. | ||||
Sequence: Missing |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000443516 | 1-293 | Protein nud-2 | |||
Sequence: MDLSEDQIRGLPHHELLGHFLQMREEFNEFQTSSAEIEKMMDSELDDLKTQLKKAETRVQQMTTEQIRNKDRQDDSRVQFAQVEEQLRRENSHLHEQCESQRERIRKLEQRNDVLETSERNKEYLASDLGSKLDHAIEKIAMLESELYERQVAAEEMHRLREEQLRTTERPRLIVEPLRNDPEILPDEPSPGPSKEEFKMSSEDVFMEDVQHHEDVRMEETIAKIDEVRIDDNKNIQEKSQRVSTGTGAGACINRIVKDLMTKVERLDSILSTIRVSNNSSNNNSSHLTTTRA |
Proteomic databases
Expression
Tissue specificity
Expressed in ventral cord neurons, the pharynx, seam cells of the hypodermis and in vulval muscle cells.
Gene expression databases
Interaction
Subunit
Component of a dynein-regulating complex composed of at least lis-1 and nud-2 (PubMed:20005871).
Interacts with lis-1; the interaction is direct (PubMed:16996038).
Interacts (via C-terminus) with unc-83; the interaction is direct, and is required for recruitment of nud-2 to the nuclear envelope (PubMed:20005871).
Interacts with lis-1; the interaction is direct (PubMed:16996038).
Interacts (via C-terminus) with unc-83; the interaction is direct, and is required for recruitment of nud-2 to the nuclear envelope (PubMed:20005871).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | O45717 | magu-4 Q20398 | 4 | EBI-326083, EBI-322716 | |
BINARY | O45717 | unc-83 Q23064-3 | 5 | EBI-326083, EBI-2902257 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for coiled coil, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Coiled coil | 36-147 | |||||
Sequence: EIEKMMDSELDDLKTQLKKAETRVQQMTTEQIRNKDRQDDSRVQFAQVEEQLRRENSHLHEQCESQRERIRKLEQRNDVLETSERNKEYLASDLGSKLDHAIEKIAMLESEL | ||||||
Region | 239-293 | Required for interaction with unc-83 isoform c | ||||
Sequence: KSQRVSTGTGAGACINRIVKDLMTKVERLDSILSTIRVSNNSSNNNSSHLTTTRA |
Sequence similarities
Belongs to the nudE family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length293
- Mass (Da)34,265
- Last updated1998-06-01 v1
- Checksum84E075C3DC96C3D9
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX284601 EMBL· GenBank· DDBJ | CAB05597.1 EMBL· GenBank· DDBJ | Genomic DNA |