O45583 · GMD2_CAEEL
- ProteinGDP-mannose 4,6 dehydratase 2
- Genegmd-2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids382 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the conversion of GDP-D-mannose to GDP-4-dehydro-6-deoxy-D-mannose.
Catalytic activity
- GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H2O
Cofactor
Temperature Dependence
Optimum temperature is 16-23 degrees Celsius.
Pathway
Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 40-45 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: GITGQD | ||||||
Binding site | 97-98 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: DM | ||||||
Binding site | 119-123 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: LAAQS | ||||||
Binding site | 134 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Active site | 166 | |||||
Sequence: T | ||||||
Active site | 168 | Nucleophile | ||||
Sequence: E | ||||||
Active site | 190 | Nucleophile | ||||
Sequence: Y | ||||||
Binding site | 194 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 220 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 225 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | GDP-mannose 4,6-dehydratase activity | |
Biological Process | 'de novo' GDP-L-fucose biosynthetic process | |
Biological Process | GDP-L-fucose biosynthetic process | |
Biological Process | GDP-mannose metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGDP-mannose 4,6 dehydratase 2
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionO45583
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000201709 | 1-382 | GDP-mannose 4,6 dehydratase 2 | |||
Sequence: MEARNAEGLESCIEKIQEVKLSSFAELKAFRERKVALITGITGQDGSYLAELLLSKGYKVHGIIRRSSSFNTARIEHLYGNPVTHNGSASFSLHYGDMTDSSCLIKLISTIEPTEIYHLAAQSHVKVSFDLPEYTAEVDAVGTLRLLDAIHACRLTEKVRFYQASTSELYGKVQEIPQSELTPFYPRSPYAVAKMYGYWIVVNYREAYKMFACNGILFNHESPRRGETFVTRKITRSVAKISLRQQEHIELGNLSALRDWGHAKEYVEAMWRILQQDTPDDFVIATGKQFSVREFCNLAFAEIGEQLVWEGEGVDEVGKNQDGVVRVKVSPKYYRPTEVETLLGNPAKARKTLGWEPKITVPELVKEMVASDIALMEADPMA |
Proteomic databases
Expression
Structure
Sequence
- Sequence statusComplete
- Length382
- Mass (Da)43,071
- Last updated1998-06-01 v1
- Checksum1A3437F87872B457
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z81553 EMBL· GenBank· DDBJ | CAB04494.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AM231685 EMBL· GenBank· DDBJ | CAJ77754.1 EMBL· GenBank· DDBJ | mRNA |