O43324 · MCA3_HUMAN

  • Protein
    Eukaryotic translation elongation factor 1 epsilon-1
  • Gene
    EEF1E1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Positive modulator of ATM response to DNA damage.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentaminoacyl-tRNA synthetase multienzyme complex
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentnucleolus
Cellular Componentnucleoplasm
Cellular Componentnucleus
Biological Processcellular response to leukemia inhibitory factor
Biological Processnegative regulation of cell population proliferation
Biological Processpositive regulation of apoptotic process
Biological Processpositive regulation of apoptotic signaling pathway
Biological Processpositive regulation of cellular senescence
Biological Processpositive regulation of DNA damage response, signal transduction by p53 class mediator
Biological Processtranslation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Eukaryotic translation elongation factor 1 epsilon-1
  • Alternative names
    • Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3
    • Elongation factor p18
    • Multisynthase complex auxiliary component p18

Gene names

    • Name
      EEF1E1
    • Synonyms
      AIMP3, P18

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo

Accessions

  • Primary accession
    O43324
  • Secondary accessions
    • C9JLK5
    • Q5THS2

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Cytoplasm, cytosol
Nucleus
Note: Cytoplasmic under growth arrest conditions. Translocated into the nucleus when growth resumes (S phase) and following DNA damage.

Keywords

Disease & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis69Disrupts interaction with MARS1.
Mutagenesis73Disrupts interaction with MARS1.
Mutagenesis144Disrupts interaction with EPRS1.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 190 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Organism-specific databases

Miscellaneous

Genetic variation databases

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain, modified residue (large scale data).

TypeIDPosition(s)SourceDescription
Initiator methionine1UniProtRemoved
Modified residue2UniProtN-acetylalanine
ChainPRO_00002211322-174UniProtEukaryotic translation elongation factor 1 epsilon-1
Modified residue (large scale data)8PRIDEPhosphoserine
Modified residue138UniProtN6-acetyllysine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Down-regulated in various cancer tissues.

Induction

By DNA damaging agents such as UV, adriamycin, actinomycin-D and cisplatin.

Gene expression databases

Organism-specific databases

Interaction

Subunit

Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18 (PubMed:19131329, PubMed:19289464).
Can interact simultaneously with MARS1 and EPRS1 (PubMed:26472928).
Forms a linear complex that contains MARS1, EEF1E1, EPRS1 and AIMP2 that is at the core of the multisubunit complex (PubMed:26472928).
Interacts with ATM and ATR. The interaction with ATM, which takes place independently of TP53, is induced by DNA damage that may occur during genotoxic stress or cell growth. The interaction with ATR is enhanced by UV irradiation

Binary interactions

View interactors in UniProtKB
View CPX-2469 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region, domain, coiled coil.

TypeIDPosition(s)Description
Region2-56N-terminal
Domain50-173GST C-terminal
Region57-63Linker
Region64-152C-terminal
Coiled coil153-169

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

O43324-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    174
  • Mass (Da)
    19,811
  • Last updated
    1998-06-01 v1
  • Checksum
    58AAE4BD9E1684E2
MAAAAELSLLEKSLGLSKGNKYSAQGERQIPVLQTNNGPSLTGLTTIAAHLVKQANKEYLLGSTAEEKAIVQQWLEYRVTQVDGHSSKNDIHTLLKDLNSYLEDKVYLTGYNFTLADILLYYGLHRFIVDLTVQEKEKYLNVSRWFCHIQHYPGIRQHLSSVVFIKNRLYTNSH

O43324-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 129-174: VDLTVQEKEKYLNVSRWFCHIQHYPGIRQHLSSVVFIKNRLYTNSH → IRKLRHTEVGN

Computationally mapped potential isoform sequences

There are 4 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
D6RBD7D6RBD7_HUMANEEF1E1150
H0YAL7H0YAL7_HUMANEEF1E1136
H0YAH9H0YAH9_HUMANEEF1E156
D6RCQ0D6RCQ0_HUMANEEF1E194

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_045088129-174in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB011079
EMBL· GenBank· DDBJ
BAA24926.1
EMBL· GenBank· DDBJ
mRNA
AF054186
EMBL· GenBank· DDBJ
AAC39916.1
EMBL· GenBank· DDBJ
mRNA
BT007306
EMBL· GenBank· DDBJ
AAP35970.1
EMBL· GenBank· DDBJ
mRNA
AL355499
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AL023694
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AL451187
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BC005291
EMBL· GenBank· DDBJ
AAH05291.1
EMBL· GenBank· DDBJ
mRNA
CK002875
EMBL· GenBank· DDBJ
-mRNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

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