O43100 · CBF5_EMENI
- ProteinH/ACA ribonucleoprotein complex subunit CBF5
- Genecbf5
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids481 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs and play a central role in ribosomal RNA processing. The H/ACA snoRNP complex also mediates pseudouridylation of other types of RNAs. Catalyzes pseudouridylation at position 93 in U2 snRNA. Also catalyzes pseudouridylation of mRNAs; H/ACA-type snoRNAs probably guide pseudouridylation of mRNAs.
Catalytic activity
- uridine in 5S rRNA = pseudouridine in 5S rRNA
- uridine in snRNA = pseudouridine in snRNA
- a uridine in mRNA = a pseudouridine in mRNA
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 96 | Nucleophile | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | box H/ACA snoRNP complex | |
Cellular Component | microtubule | |
Molecular Function | DNA binding | |
Molecular Function | pseudouridine synthase activity | |
Molecular Function | pseudouridylate synthase activity | |
Molecular Function | RNA binding | |
Molecular Function | snRNA pseudouridine synthase activity | |
Biological Process | anisotropic cell growth | |
Biological Process | box H/ACA sno(s)RNA 3'-end processing | |
Biological Process | conidium formation | |
Biological Process | endocytosis | |
Biological Process | establishment of cell polarity | |
Biological Process | mRNA pseudouridine synthesis | |
Biological Process | rRNA pseudouridine synthesis | |
Biological Process | snRNA pseudouridine synthesis |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameH/ACA ribonucleoprotein complex subunit CBF5
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Nidulantes
Accessions
- Primary accessionO43100
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000121979 | 1-481 | H/ACA ribonucleoprotein complex subunit CBF5 | |||
Sequence: MAKEVDYTIKPEATASNINTEDWPLLLKNYDKLMVRTGHFTPIPAGSSPLKRDLKSYINSGVINLDKPSNPSSHEVVAWMKRILRAEKTGHSGTLDPKVTGCLIVCIDRATRLVKSQQGAGKEYVCVIRLHDKIPGGEAQFKRALETLTGALFQRPPLISAVKRQLRIRTIHESKLYEFDNERHLGVFWVSCEAGTYIRTLCVHLGLLLGVGAHMQELRRVRSGAMSENEGMVTLHDVLDAQWLYDNQRDESYLRKVIKPLESLLTTYKRIVVKDSAVNAVCYGAKLMIPGLLRFEAGIELGEEVVLMTTKGEAIAIGIAQMSTVELSTCDHGVVAKVKRCIMERDLYPRRWGLGPVALEKKKLKSSGKLDKYGRANEATPAKWKSEYKDYSAPDGDSSQQAVDVVAKEEASPKEEPSLEANESKMDIDDAQDDEDKKKRKRHEGETPEERAERKRKKKEKKEKKERRKSKQEKDDSDDSD |
Interaction
Subunit
Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 268-343 | PUA | ||||
Sequence: YKRIVVKDSAVNAVCYGAKLMIPGLLRFEAGIELGEEVVLMTTKGEAIAIGIAQMSTVELSTCDHGVVAKVKRCIM | ||||||
Region | 387-481 | Disordered | ||||
Sequence: EYKDYSAPDGDSSQQAVDVVAKEEASPKEEPSLEANESKMDIDDAQDDEDKKKRKRHEGETPEERAERKRKKKEKKEKKERRKSKQEKDDSDDSD | ||||||
Compositional bias | 408-456 | Basic and acidic residues | ||||
Sequence: KEEASPKEEPSLEANESKMDIDDAQDDEDKKKRKRHEGETPEERAERKR | ||||||
Compositional bias | 466-481 | Basic and acidic residues | ||||
Sequence: ERRKSKQEKDDSDDSD |
Sequence similarities
Belongs to the pseudouridine synthase TruB family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length481
- Mass (Da)54,204
- Last updated1998-06-01 v1
- ChecksumA671BB6C57CE9590
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 408-456 | Basic and acidic residues | ||||
Sequence: KEEASPKEEPSLEANESKMDIDDAQDDEDKKKRKRHEGETPEERAERKR | ||||||
Compositional bias | 466-481 | Basic and acidic residues | ||||
Sequence: ERRKSKQEKDDSDDSD |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U59148 EMBL· GenBank· DDBJ | AAB94296.1 EMBL· GenBank· DDBJ | mRNA | ||
AY057454 EMBL· GenBank· DDBJ | AAL23694.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AACD01000163 EMBL· GenBank· DDBJ | EAA60139.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
BN001303 EMBL· GenBank· DDBJ | CBF77883.1 EMBL· GenBank· DDBJ | Genomic DNA |