O41933 · MIR1_MHV68

Function

function

E3 ubiquitin-protein ligase that mediates ubiquitination of host surface class I (MHC-I) H-2D(b)/H2-D1 and H-2K(b)/H2-K1 molecules before they exit the endoplasmic reticulum, leading to their degradation by the endoplasmic reticulum-associated degradation (ERAD) system, thus blocking the immune detection of virus-infected cells. Mediates ubiquitination of lysine, as well as serine and threonine residues present in the cytoplasmic tail of surface class I molecules. Promotes ubiquitination of hydroxylated serine or threonine residues via ester bonds instead of the classical isopeptide linkage.

Miscellaneous

Murine herpesvirus 76 (MHV76) is identical to murine herpesvirus 68 except for a large deletion at the genome left end of the unique region.

Catalytic activity

  • [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine.
    EC:2.3.2.36 (UniProtKB | ENZYME | Rhea)

Pathway

Protein modification; protein ubiquitination.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site8Zn2+ 1 (UniProtKB | ChEBI)
Binding site11Zn2+ 1 (UniProtKB | ChEBI)
Binding site22Zn2+ 2 (UniProtKB | ChEBI)
Binding site24Zn2+ 2 (UniProtKB | ChEBI)
Binding site32Zn2+ 1 (UniProtKB | ChEBI)
Binding site35Zn2+ 1 (UniProtKB | ChEBI)
Binding site48Zn2+ 2 (UniProtKB | ChEBI)
Binding site51Zn2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenthost cell endoplasmic reticulum membrane
Cellular Componentmembrane
Molecular Functiontransferase activity
Molecular Functionzinc ion binding
Biological Processprotein ubiquitination
Biological Processsymbiont-mediated suppression of host antigen processing and presentation of peptide antigen via MHC class I
Biological Processvirus-mediated perturbation of host defense response

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    E3 ubiquitin-protein ligase MIR1
  • EC number
  • Alternative names
    • MK3
    • Modulator of immune recognition 1 homolog
    • ORF K3
    • RING-type E3 ubiquitin transferase MIR1

Gene names

    • Name
      K3

Organism names

Accessions

  • Primary accession
    O41933

Proteomes

Subcellular Location

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-81Cytoplasmic
Transmembrane82-102Helical
Topological domain103-113Extracellular
Transmembrane114-134Helical
Topological domain135-201Cytoplasmic

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis9Abolishes E3 ligase activity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002213911-201E3 ubiquitin-protein ligase MIR1

Expression

Keywords

Interaction

Subunit

Interacts with host UBE2J2.

Structure

3D structure databases

Family & Domains

Features

Showing features for zinc finger, region.

TypeIDPosition(s)Description
Zinc finger1-58RING-CH-type
Region52-79DIRT

Domain

The DIRT (domain in between the RING-CH-type and the first transmembrane) region is essential to determine substrate residues that can be ubiquitinated.
The RING-CH-type zinc finger (also named RINGV-type zinc-finger) mediates the E3 ubiquitin ligase activity.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    201
  • Mass (Da)
    22,214
  • Last updated
    1998-01-01 v1
  • Checksum
    0953B5BDACDF8367
MDSTGEFCWICHQPEGPLKRFCGCKGSCAVSHQDCLRGWLETSRRQTCALCGTPYSMKWKTKPLREWTWGEEEVLAAMEACLPLVLIPLAVLMIVMGTWLLVNHNGFLSPRMQVVLVVIVLLAMIVFSASASYVMVEGPGCLDTCTAKNSTVTVNSIDEAIATQQPTKTDLGLARETLSTRFRRGKCRSCCRLGCVRLCCV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U97553
EMBL· GenBank· DDBJ
AAB66429.1
EMBL· GenBank· DDBJ
Genomic DNA
AF105037
EMBL· GenBank· DDBJ
AAF19280.1
EMBL· GenBank· DDBJ
Genomic DNA
AF324455
EMBL· GenBank· DDBJ
AAK16705.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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