O41933 · MIR1_MHV68
- ProteinE3 ubiquitin-protein ligase MIR1
- GeneK3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids201 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
E3 ubiquitin-protein ligase that mediates ubiquitination of host surface class I (MHC-I) H-2D(b)/H2-D1 and H-2K(b)/H2-K1 molecules before they exit the endoplasmic reticulum, leading to their degradation by the endoplasmic reticulum-associated degradation (ERAD) system, thus blocking the immune detection of virus-infected cells. Mediates ubiquitination of lysine, as well as serine and threonine residues present in the cytoplasmic tail of surface class I molecules. Promotes ubiquitination of hydroxylated serine or threonine residues via ester bonds instead of the classical isopeptide linkage.
Miscellaneous
Murine herpesvirus 76 (MHV76) is identical to murine herpesvirus 68 except for a large deletion at the genome left end of the unique region.
Catalytic activity
Pathway
Protein modification; protein ubiquitination.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 8 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 11 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 22 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 24 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 32 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 35 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 48 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 51 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell endoplasmic reticulum membrane | |
Cellular Component | membrane | |
Molecular Function | transferase activity | |
Molecular Function | zinc ion binding | |
Biological Process | protein ubiquitination | |
Biological Process | symbiont-mediated suppression of host antigen processing and presentation of peptide antigen via MHC class I | |
Biological Process | virus-mediated perturbation of host defense response |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameE3 ubiquitin-protein ligase MIR1
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Peploviricota > Herviviricetes > Herpesvirales > Orthoherpesviridae > Gammaherpesvirinae > Rhadinovirus > Rhadinovirus muridgamma4
- Virus hosts
Accessions
- Primary accessionO41933
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Host endoplasmic reticulum membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-81 | Cytoplasmic | ||||
Sequence: MDSTGEFCWICHQPEGPLKRFCGCKGSCAVSHQDCLRGWLETSRRQTCALCGTPYSMKWKTKPLREWTWGEEEVLAAMEAC | ||||||
Transmembrane | 82-102 | Helical | ||||
Sequence: LPLVLIPLAVLMIVMGTWLLV | ||||||
Topological domain | 103-113 | Extracellular | ||||
Sequence: NHNGFLSPRMQ | ||||||
Transmembrane | 114-134 | Helical | ||||
Sequence: VVLVVIVLLAMIVFSASASYV | ||||||
Topological domain | 135-201 | Cytoplasmic | ||||
Sequence: MVEGPGCLDTCTAKNSTVTVNSIDEAIATQQPTKTDLGLARETLSTRFRRGKCRSCCRLGCVRLCCV |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 9 | Abolishes E3 ligase activity. | ||||
Sequence: W → R |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000221391 | 1-201 | E3 ubiquitin-protein ligase MIR1 | |||
Sequence: MDSTGEFCWICHQPEGPLKRFCGCKGSCAVSHQDCLRGWLETSRRQTCALCGTPYSMKWKTKPLREWTWGEEEVLAAMEACLPLVLIPLAVLMIVMGTWLLVNHNGFLSPRMQVVLVVIVLLAMIVFSASASYVMVEGPGCLDTCTAKNSTVTVNSIDEAIATQQPTKTDLGLARETLSTRFRRGKCRSCCRLGCVRLCCV |
Expression
Keywords
- Developmental stage
Interaction
Subunit
Interacts with host UBE2J2.
Family & Domains
Features
Showing features for zinc finger, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Zinc finger | 1-58 | RING-CH-type | ||||
Sequence: MDSTGEFCWICHQPEGPLKRFCGCKGSCAVSHQDCLRGWLETSRRQTCALCGTPYSMK | ||||||
Region | 52-79 | DIRT | ||||
Sequence: GTPYSMKWKTKPLREWTWGEEEVLAAME |
Domain
The DIRT (domain in between the RING-CH-type and the first transmembrane) region is essential to determine substrate residues that can be ubiquitinated.
The RING-CH-type zinc finger (also named RINGV-type zinc-finger) mediates the E3 ubiquitin ligase activity.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length201
- Mass (Da)22,214
- Last updated1998-01-01 v1
- Checksum0953B5BDACDF8367
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U97553 EMBL· GenBank· DDBJ | AAB66429.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF105037 EMBL· GenBank· DDBJ | AAF19280.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF324455 EMBL· GenBank· DDBJ | AAK16705.1 EMBL· GenBank· DDBJ | Genomic DNA |