O36966 · POLN_DCVEB
- ProteinReplicase polyprotein
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1759 (go to sequence)
- Protein existencePredicted
- Annotation score5/5
Function
function
Protein 1A functions as a suppressor of RNA-mediated gene silencing, an antiviral defense mechanism of insect cells. Binds to long dsRNA and to a lesser extent, to siRNA.
RNA-directed RNA polymerase replicates genomic and antigenomic RNA.
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Features
Showing features for site, binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 97-98 | Cleavage; by ribosomal skip | ||||
Sequence: GP | ||||||
Binding site | 449-456 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GESGVGKT | ||||||
Site | 701-702 | Cleavage; by 3C-like protease | ||||
Sequence: QK | ||||||
Site | 818-819 | Cleavage; by 3C-like protease | ||||
Sequence: EI | ||||||
Site | 901-902 | Cleavage; by 3C-like protease | ||||
Sequence: LS | ||||||
Active site | 994 | For picornain 3C-like protease activity | ||||
Sequence: H | ||||||
Active site | 1054 | For picornain 3C-like protease activity | ||||
Sequence: D | ||||||
Active site | 1152 | For picornain 3C-like protease activity | ||||
Sequence: C | ||||||
Site | 1209-1210 | Cleavage; by 3C-like protease | ||||
Sequence: QF |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | host cell cytosol | |
Cellular Component | host cell nucleus | |
Molecular Function | ATP binding | |
Molecular Function | cysteine-type endopeptidase activity | |
Molecular Function | lncRNA binding | |
Molecular Function | RNA helicase activity | |
Molecular Function | RNA-dependent RNA polymerase activity | |
Biological Process | DNA-templated transcription | |
Biological Process | proteolysis | |
Biological Process | symbiont-mediated suppression of host innate immune response | |
Biological Process | viral RNA genome replication | |
Biological Process | virus-mediated perturbation of host defense response |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameReplicase polyprotein
- Cleaved into 8 chains
Gene names
Organism names
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Picornavirales > Dicistroviridae > Cripavirus > Cripavirus drosophilae
- Virus hosts
Accessions
- Primary accessionO36966
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000399917 | ?-901 | Protein 3B | |||
Sequence: MESDKSMACLNRILMNKMMFVEDKISTLKMVADYYQKEVKYDFDAVESPREAPVFRCTCRFLGYTIMTQGIGKKNPKQEAARQMLLLLSGDVETNPGPVQSRPVYYRYNDPRYTRLEKAIERRDDKIKTLIKELRRQIKNRKIYSQGMFDKLTKQISDGIKDGVGSEQMNGNLTRICDFLENTLPGLQANIQATVIDTTDKYVSLKEDIMKIVLVILLVRLLMVWKKYRASLCVILIFIFKFYGFDQKLIDLIMDLKNKIFSQGALEDTVEEVVYHPWFHTCGKIIFAVMAFLTIKKIPGKQDWDSYITRLDRIPKSIEGAKKITDYCSEYFNIANDQIKMMVLGKTKEELQRANGLYGEIQAWAQEVRQYLELDQRNKIDLDTETANRVEQLWIKGLKFKSEPLLSKEMSALVHTTLLPAKQLYEYVSCSPVKGGGPRMRPICLWLVGESGVGKTEMVYPLCIDVLREMGMIKKDDFHHQVYGRQVETEFWDGYKGQKIVIYDDAFQKKDDKTAANPEIFEVIRSCNTFPQHLHMAALHDKNTFSAAELLLYTTNDYNVKLESITFPDAFFNRMGDMAYKVSPKKEYGIETEKGNSGKTYLKLDKSKLDKTKAIDLSVYEFQKIVRDEKSDAGWIDSGSPLDYEDFAKLVCSKWKEAKQSSMNKLKFLEEYAIRAQVGSEENSEYGDCIDFVDDIAKRLQKGETLEEIEFDYASDPEMFTQYYHFKSTIKPASRWQKYKDRMDICLSDCKTYLAKKYEEIKKILAEHPILTILGMIGVALSALAMYYWFSKSLDPVEAEVAPSGDAKTVRLPRKLVEIGASGDVKTQKIVKPVVETEWHRNNKGEIEISCDECGMHRMSAFNNMTDEEFDNCTYEDLNKDQKRELAQWSTKDSWLGRFFL | ||||||
Chain | PRO_0000399912 | 1-97 | Protein 1A | |||
Sequence: MESDKSMACLNRILMNKMMFVEDKISTLKMVADYYQKEVKYDFDAVESPREAPVFRCTCRFLGYTIMTQGIGKKNPKQEAARQMLLLLSGDVETNPG | ||||||
Chain | PRO_0000399913 | 1-121 | Protein 2A | |||
Sequence: MESDKSMACLNRILMNKMMFVEDKISTLKMVADYYQKEVKYDFDAVESPREAPVFRCTCRFLGYTIMTQGIGKKNPKQEAARQMLLLLSGDVETNPGPVQSRPVYYRYNDPRYTRLEKAIE | ||||||
Chain | PRO_0000399911 | 1-1759 | Replicase polyprotein | |||
Sequence: MESDKSMACLNRILMNKMMFVEDKISTLKMVADYYQKEVKYDFDAVESPREAPVFRCTCRFLGYTIMTQGIGKKNPKQEAARQMLLLLSGDVETNPGPVQSRPVYYRYNDPRYTRLEKAIERRDDKIKTLIKELRRQIKNRKIYSQGMFDKLTKQISDGIKDGVGSEQMNGNLTRICDFLENTLPGLQANIQATVIDTTDKYVSLKEDIMKIVLVILLVRLLMVWKKYRASLCVILIFIFKFYGFDQKLIDLIMDLKNKIFSQGALEDTVEEVVYHPWFHTCGKIIFAVMAFLTIKKIPGKQDWDSYITRLDRIPKSIEGAKKITDYCSEYFNIANDQIKMMVLGKTKEELQRANGLYGEIQAWAQEVRQYLELDQRNKIDLDTETANRVEQLWIKGLKFKSEPLLSKEMSALVHTTLLPAKQLYEYVSCSPVKGGGPRMRPICLWLVGESGVGKTEMVYPLCIDVLREMGMIKKDDFHHQVYGRQVETEFWDGYKGQKIVIYDDAFQKKDDKTAANPEIFEVIRSCNTFPQHLHMAALHDKNTFSAAELLLYTTNDYNVKLESITFPDAFFNRMGDMAYKVSPKKEYGIETEKGNSGKTYLKLDKSKLDKTKAIDLSVYEFQKIVRDEKSDAGWIDSGSPLDYEDFAKLVCSKWKEAKQSSMNKLKFLEEYAIRAQVGSEENSEYGDCIDFVDDIAKRLQKGETLEEIEFDYASDPEMFTQYYHFKSTIKPASRWQKYKDRMDICLSDCKTYLAKKYEEIKKILAEHPILTILGMIGVALSALAMYYWFSKSLDPVEAEVAPSGDAKTVRLPRKLVEIGASGDVKTQKIVKPVVETEWHRNNKGEIEISCDECGMHRMSAFNNMTDEEFDNCTYEDLNKDQKRELAQWSTKDSWLGRFFLSRDRKNKVGIWAEVGQSGDVKTNKAQIKRVEAGAEELVTVALTQGCSDDAAHNLMIDVFQKNTYRMSYFRGDKRYQLGNCTFVRGWSFIMPYHFVQAVFARRLPPNTIISLSQQMSEDLMQIPLSHFFSAGVDNFYLTDNCVRLPFKNGDFRDCVMVNLHSRMCTPHRDLVRHFILTSDQGKLKGSFSGAMATFHVNNMGLYRVYNWLNAVRPCDKKIEIFHPEDGFEYPEESYIQRDCYEYNAPTRTGDCGSIIGLYNKYLERKIIGMHIAGNDAEEHGYACPLTQECLETAFSALVNKNKKNISSQFYYEIPNMVDPLGDSSVPEGKFYALGKSSIRVGQAVNSSIIPSRIYGKLSVPTMKPALLKPTILNNKVHNPLLSGLKKCGVDTAVLSDDEVLSASQDVCRVMLNQYNKNLNKTKYQRILTYEEAIRGTQDDEFMCAINRTTSPGFPYAQMKRNAPGKQQWMGFGEEFDFTSNYALALRKDVEQLIEDCASGKISNVIFVDTLKDERRDIAKVNVGKTRVFSAGPQHFVVAFRQYFLPFAAWLMHNRISNEVAVGTNVYSSDWERIAKRLKTKGSHVIAGDFGNFDGSLVAQILWAIFWEIFVVWLKQFIDIENSEGKRILCICLGLWSHLVHSVHIYEDNVYMWTHSQPSGNPFTVIINCLYNSIIMRLSWIRVMEKFQPRLKSMKWFNEYVALITYGDDNVLNIDAKVVEWFNQINISEVMTEMRHEYTDEAKTGDIVKSRKLEDIFFLKRKFRFSPELQRHVAPLKIEVIYEMLNWSRRSIDPDEILMSNIETAFREVVYHGKEEYDKLRSAVLALKVPQELPENPQILTYNQYLHDIEYLADPLYDF | ||||||
Chain | PRO_0000399914 | 122-263 | Protein 2B | |||
Sequence: RRDDKIKTLIKELRRQIKNRKIYSQGMFDKLTKQISDGIKDGVGSEQMNGNLTRICDFLENTLPGLQANIQATVIDTTDKYVSLKEDIMKIVLVILLVRLLMVWKKYRASLCVILIFIFKFYGFDQKLIDLIMDLKNKIFSQ | ||||||
Chain | PRO_0000399915 | 264-701 | Protein 2C | |||
Sequence: GALEDTVEEVVYHPWFHTCGKIIFAVMAFLTIKKIPGKQDWDSYITRLDRIPKSIEGAKKITDYCSEYFNIANDQIKMMVLGKTKEELQRANGLYGEIQAWAQEVRQYLELDQRNKIDLDTETANRVEQLWIKGLKFKSEPLLSKEMSALVHTTLLPAKQLYEYVSCSPVKGGGPRMRPICLWLVGESGVGKTEMVYPLCIDVLREMGMIKKDDFHHQVYGRQVETEFWDGYKGQKIVIYDDAFQKKDDKTAANPEIFEVIRSCNTFPQHLHMAALHDKNTFSAAELLLYTTNDYNVKLESITFPDAFFNRMGDMAYKVSPKKEYGIETEKGNSGKTYLKLDKSKLDKTKAIDLSVYEFQKIVRDEKSDAGWIDSGSPLDYEDFAKLVCSKWKEAKQSSMNKLKFLEEYAIRAQVGSEENSEYGDCIDFVDDIAKRLQ | ||||||
Chain | PRO_0000399916 | 702-? | Protein 3A | |||
Sequence: MESDKSMACLNRILMNKMMFVEDKISTLKMVADYYQKEVKYDFDAVESPREAPVFRCTCRFLGYTIMTQGIGKKNPKQEAARQMLLLLSGDVETNPGPVQSRPVYYRYNDPRYTRLEKAIERRDDKIKTLIKELRRQIKNRKIYSQGMFDKLTKQISDGIKDGVGSEQMNGNLTRICDFLENTLPGLQANIQATVIDTTDKYVSLKEDIMKIVLVILLVRLLMVWKKYRASLCVILIFIFKFYGFDQKLIDLIMDLKNKIFSQGALEDTVEEVVYHPWFHTCGKIIFAVMAFLTIKKIPGKQDWDSYITRLDRIPKSIEGAKKITDYCSEYFNIANDQIKMMVLGKTKEELQRANGLYGEIQAWAQEVRQYLELDQRNKIDLDTETANRVEQLWIKGLKFKSEPLLSKEMSALVHTTLLPAKQLYEYVSCSPVKGGGPRMRPICLWLVGESGVGKTEMVYPLCIDVLREMGMIKKDDFHHQVYGRQVETEFWDGYKGQKIVIYDDAFQKKDDKTAANPEIFEVIRSCNTFPQHLHMAALHDKNTFSAAELLLYTTNDYNVKLESITFPDAFFNRMGDMAYKVSPKKEYGIETEKGNSGKTYLKLDKSKLDKTKAIDLSVYEFQKIVRDEKSDAGWIDSGSPLDYEDFAKLVCSKWKEAKQSSMNKLKFLEEYAIRAQVGSEENSEYGDCIDFVDDIAKRLQ | ||||||
Chain | PRO_0000399918 | 902-1209 | 3C-like protease | |||
Sequence: SRDRKNKVGIWAEVGQSGDVKTNKAQIKRVEAGAEELVTVALTQGCSDDAAHNLMIDVFQKNTYRMSYFRGDKRYQLGNCTFVRGWSFIMPYHFVQAVFARRLPPNTIISLSQQMSEDLMQIPLSHFFSAGVDNFYLTDNCVRLPFKNGDFRDCVMVNLHSRMCTPHRDLVRHFILTSDQGKLKGSFSGAMATFHVNNMGLYRVYNWLNAVRPCDKKIEIFHPEDGFEYPEESYIQRDCYEYNAPTRTGDCGSIIGLYNKYLERKIIGMHIAGNDAEEHGYACPLTQECLETAFSALVNKNKKNISSQ | ||||||
Chain | PRO_0000399919 | 1210-1759 | RNA-directed RNA polymerase 3D-POL | |||
Sequence: FYYEIPNMVDPLGDSSVPEGKFYALGKSSIRVGQAVNSSIIPSRIYGKLSVPTMKPALLKPTILNNKVHNPLLSGLKKCGVDTAVLSDDEVLSASQDVCRVMLNQYNKNLNKTKYQRILTYEEAIRGTQDDEFMCAINRTTSPGFPYAQMKRNAPGKQQWMGFGEEFDFTSNYALALRKDVEQLIEDCASGKISNVIFVDTLKDERRDIAKVNVGKTRVFSAGPQHFVVAFRQYFLPFAAWLMHNRISNEVAVGTNVYSSDWERIAKRLKTKGSHVIAGDFGNFDGSLVAQILWAIFWEIFVVWLKQFIDIENSEGKRILCICLGLWSHLVHSVHIYEDNVYMWTHSQPSGNPFTVIINCLYNSIIMRLSWIRVMEKFQPRLKSMKWFNEYVALITYGDDNVLNIDAKVVEWFNQINISEVMTEMRHEYTDEAKTGDIVKSRKLEDIFFLKRKFRFSPELQRHVAPLKIEVIYEMLNWSRRSIDPDEILMSNIETAFREVVYHGKEEYDKLRSAVLALKVPQELPENPQILTYNQYLHDIEYLADPLYDF |
Post-translational modification
Protein 1A might be expressed through a ribosomal skip from one codon to the next without formation of a peptide bond.
Family & Domains
Features
Showing features for domain, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 23-90 | DRBM | ||||
Sequence: DKISTLKMVADYYQKEVKYDFDAVESPREAPVFRCTCRFLGYTIMTQGIGKKNPKQEAARQMLLLLSG | ||||||
Coiled coil | 113-140 | |||||
Sequence: YTRLEKAIERRDDKIKTLIKELRRQIKN | ||||||
Domain | 421-595 | SF3 helicase | ||||
Sequence: AKQLYEYVSCSPVKGGGPRMRPICLWLVGESGVGKTEMVYPLCIDVLREMGMIKKDDFHHQVYGRQVETEFWDGYKGQKIVIYDDAFQKKDDKTAANPEIFEVIRSCNTFPQHLHMAALHDKNTFSAAELLLYTTNDYNVKLESITFPDAFFNRMGDMAYKVSPKKEYGIETEKG | ||||||
Domain | 950-1191 | Peptidase C3 | ||||
Sequence: DAAHNLMIDVFQKNTYRMSYFRGDKRYQLGNCTFVRGWSFIMPYHFVQAVFARRLPPNTIISLSQQMSEDLMQIPLSHFFSAGVDNFYLTDNCVRLPFKNGDFRDCVMVNLHSRMCTPHRDLVRHFILTSDQGKLKGSFSGAMATFHVNNMGLYRVYNWLNAVRPCDKKIEIFHPEDGFEYPEESYIQRDCYEYNAPTRTGDCGSIIGLYNKYLERKIIGMHIAGNDAEEHGYACPLTQECL | ||||||
Domain | 1483-1622 | RdRp catalytic | ||||
Sequence: SHVIAGDFGNFDGSLVAQILWAIFWEIFVVWLKQFIDIENSEGKRILCICLGLWSHLVHSVHIYEDNVYMWTHSQPSGNPFTVIINCLYNSIIMRLSWIRVMEKFQPRLKSMKWFNEYVALITYGDDNVLNIDAKVVEWF |
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,759
- Mass (Da)202,942
- Last updated1998-01-01 v1
- Checksum49C391F9B0C1AAA9
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF014388 EMBL· GenBank· DDBJ | AAC58807.1 EMBL· GenBank· DDBJ | Genomic RNA |