O35889 · AFAD_RAT

Function

function

Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs) (PubMed:9334353).
Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton (PubMed:9334353).
May play a key role in the organization of epithelial structures of the embryonic ectoderm (By similarity).
Essential for the organization of adherens junctions (By similarity).

Miscellaneous

Isoform 1 increases the viscosity of F-actin in a dose-dependent manner. Isoform 1 causes F-actin to associate into bundles and meshworks.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentadherens junction
Cellular Componentapical junction complex
Cellular Componentapical part of cell
Cellular Componentaxon
Cellular Componentcell junction
Cellular Componentcell-cell contact zone
Cellular Componentcell-cell junction
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentexcitatory synapse
Cellular Componentglutamatergic synapse
Cellular Componenthippocampal mossy fiber to CA3 synapse
Cellular Componentpore complex
Cellular Componentpostsynaptic density membrane
Cellular Componentpresynaptic active zone membrane
Cellular Componentsomatodendritic compartment
Cellular Componenttight junction
Molecular Functionactin filament binding
Molecular Functioncell adhesion molecule binding
Molecular FunctionLIM domain binding
Molecular Functionsmall GTPase binding
Biological Processadherens junction maintenance
Biological Processbicellular tight junction assembly
Biological Processbrain morphogenesis
Biological Processcell-cell adhesion mediated by cadherin
Biological Processcerebral cortex development
Biological Processdendrite arborization
Biological Processestablishment of endothelial intestinal barrier
Biological Processestablishment of protein localization to plasma membrane
Biological Processhomeostasis of number of cells
Biological Processnegative regulation of cell migration
Biological Processneuroepithelial cell differentiation
Biological Processpore complex assembly
Biological Processpositive regulation of cell-cell adhesion
Biological Processpositive regulation of cell-cell adhesion mediated by cadherin
Biological Processpositive regulation of dendrite extension
Biological Processpositive regulation of dendrite morphogenesis
Biological Processpositive regulation of dendritic spine morphogenesis
Biological Processpositive regulation of gene expression
Biological Processpositive regulation of mini excitatory postsynaptic potential
Biological Processpostsynaptic specialization organization
Biological Processprotein localization to cell junction
Biological Processradial glial cell differentiation
Biological Processregulation of oligodendrocyte progenitor proliferation
Biological Processregulation of postsynapse assembly
Biological Processregulation of protein localization
Biological Processsignal transduction
Biological Processtelencephalon development

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Afadin
  • Alternative names
    • Afadin adherens junction formation factor
    • Protein Af-6

Gene names

    • Name
      Afdn
    • Synonyms
      Af6, Mllt4

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    O35889
  • Secondary accessions
    • O35890

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00002159201-1829Afadin
Modified residue216Phosphoserine
Modified residue246Phosphoserine
Modified residue256Phosphoserine
Modified residue391Phosphoserine
Modified residue424Phosphoserine
Modified residue512Phosphoserine
Modified residue557Phosphoserine
Modified residue562Phosphoserine
Modified residue589Phosphoserine
Modified residue655Phosphoserine
Modified residue1090Phosphoserine
Modified residue1114Phosphoserine
Modified residue1133Phosphoserine
Modified residue1147Phosphoserine
Modified residue1150Phosphoserine
Modified residue1179Phosphoserine
Modified residue1180Phosphoserine
Modified residue1189Phosphoserine
Modified residue1206Phosphoserine
Modified residue1218Phosphothreonine
Modified residue1239Phosphothreonine
Modified residue1245Phosphoserine
Modified residue1282Phosphoserine
Modified residue1335Phosphoserine
Modified residue1337Phosphothreonine
Modified residue1506Phosphoserine
Modified residue1517Phosphoserine
Modified residue1701Phosphoserine
Modified residue1726Phosphoserine
Modified residue1779Phosphoserine
Modified residue1804Phosphoserine
Modified residue1812N6-acetyllysine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Isoform 1 is widely expressed, including in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. Isoform 2 is mainly expressed in the brain.

Interaction

Subunit

Homodimer. Interacts with F-actin, nectin and NECTIN3. Essential for the association of nectin and E-cadherin. Isoform 2/s-afadin does not interact with F-actin. Interacts with ZO-1 and occludin, but probably in an indirect manner. Interacts with RIT1, RIT2, NRXN1 and BCR (By similarity).
Interacts with ADAM10; the interaction locks ADAM10 at adherens junctions following ADAM10 recruitment to adherens junctions by TSPAN33 (PubMed:30463011).

Binary interactions

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region, compositional bias, coiled coil.

TypeIDPosition(s)Description
Domain39-133Ras-associating 1
Region129-196Disordered
Compositional bias143-196Basic and acidic residues
Coiled coil146-186
Domain246-348Ras-associating 2
Region356-377Disordered
Domain441-507FHA
Region539-595Disordered
Compositional bias545-568Polar residues
Compositional bias574-593Basic and acidic residues
Domain668-915Dilute
Domain1014-1100PDZ
Region1114-1230Disordered
Compositional bias1137-1154Polar residues
Compositional bias1161-1176Basic and acidic residues
Compositional bias1178-1216Polar residues
Region1300-1533Disordered
Compositional bias1310-1347Polar residues
Compositional bias1367-1382Pro residues
Compositional bias1412-1448Basic and acidic residues
Coiled coil1417-1454
Compositional bias1449-1497Polar residues
Compositional bias1519-1533Basic and acidic residues
Coiled coil1530-1564
Region1574-1724Disordered
Compositional bias1595-1685Basic and acidic residues
Coiled coil1600-1672
Compositional bias1687-1716Pro residues
Region1742-1829Disordered
Compositional bias1751-1765Polar residues
Compositional bias1811-1829Basic and acidic residues

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

O35889-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Name
    1
  • Synonyms
    l-afadin, p205
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Length
    1,829
  • Mass (Da)
    207,678
  • Last updated
    1998-01-01 v1
  • Checksum
    45C597A82F109D6F
MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKEERPSQGDDSENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAESLEKYGLEKENPKDYCIARVMLPPGAQHSDERGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDYIPKKMKKHVEGKPLKGKDRADGSGYGSALPPEKLPYLVELSPGRRNHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVDGQRISETTMLQSGMRLQFGTSHVFKFVDPIQDHVLSKRSVDGGLMVKGPRHKPGAVQETTFELGGDIHSGTALPASRSTTRLDSDRVSSASSTAERGMVKPMIRLDQEQDYRRRESRTQDAAGPELMLPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQHRPDISPTERTHKAIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHTRSPGTWTIYFEGADYESHLMRENTELTQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPQMPWTEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSPYTSGTAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPVQNQWPNYEEKPHMHTESDHASIAIQRVTRSQEELREEKVYQLERHRVESGMDRKCDSDMWINQSSSVESSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAVLPTPVAVSQPIRTDLPPPPPPPPAHYTSDFDGISMDLPLPPPPANQAAPQSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPASFSPLATQAKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITISKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSKEIHELQNKGDRTAEESDRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREVEDRVRQEEDGRHQEEERVKRDAEEKRRQEEGYYSRLEAERRRQHEEAARRLLEPEEPGLSRPPLPQDYEPPSQSSAPSAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYDDDDEEENYVPAGPNSYSGSAGTTAGTYDAPRDTREKLSRSQDADLPGSSGAPENLTFRERQRLFSQGQDVSDKVKASRKLTELENELNTK

O35889-2

Computationally mapped potential isoform sequences

There are 4 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8I6AL62A0A8I6AL62_RATAfdn1758
A0A8I5ZNL2A0A8I5ZNL2_RATAfdn1772
A0A8I5ZLU6A0A8I5ZLU6_RATAfdn1885
F1LT10F1LT10_RATAfdn1670

Features

Showing features for compositional bias, alternative sequence.

TypeIDPosition(s)Description
Compositional bias143-196Basic and acidic residues
Compositional bias545-568Polar residues
Compositional bias574-593Basic and acidic residues
Alternative sequenceVSP_011726679-685in isoform 2
Compositional bias1137-1154Polar residues
Compositional bias1161-1176Basic and acidic residues
Compositional bias1178-1216Polar residues
Compositional bias1310-1347Polar residues
Compositional bias1367-1382Pro residues
Compositional bias1412-1448Basic and acidic residues
Compositional bias1449-1497Polar residues
Compositional bias1519-1533Basic and acidic residues
Compositional bias1595-1685Basic and acidic residues
Alternative sequenceVSP_0117271609in isoform 2
Alternative sequenceVSP_0117281655-1658in isoform 2
Alternative sequenceVSP_0117291659-1829in isoform 2
Compositional bias1687-1716Pro residues
Compositional bias1751-1765Polar residues
Compositional bias1811-1829Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U83231
EMBL· GenBank· DDBJ
AAC53391.1
EMBL· GenBank· DDBJ
mRNA
U83230
EMBL· GenBank· DDBJ
AAC53390.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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