O35458 · VIAAT_RAT
- ProteinVesicular inhibitory amino acid transporter
- GeneSlc32a1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids525 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Antiporter that exchanges vesicular protons for cytosolic 4-aminobutanoate or to a lesser extend glycine, thus allowing their secretion from nerve terminals (By similarity) (PubMed:9349821).
The transport is equally dependent on the chemical and electrical components of the proton gradient (PubMed:9349821).
May also transport beta-alanine (PubMed:23919636).
Acidification of GABAergic synaptic vesicles is a prerequisite for 4-aminobutanoate uptake (By similarity).
The transport is equally dependent on the chemical and electrical components of the proton gradient (PubMed:9349821).
May also transport beta-alanine (PubMed:23919636).
Acidification of GABAergic synaptic vesicles is a prerequisite for 4-aminobutanoate uptake (By similarity).
Catalytic activity
- beta-alanine(out) + n H+(in) = beta-alanine(in) + n H+(out)β-alanine (out)CHEBI:57966
+ n H+ (in)CHEBI:15378= β-alanine (in)CHEBI:57966+ n H+ (out)CHEBI:15378 - 4-aminobutanoate(out) + n H+(in) = 4-aminobutanoate(in) + n H+(out)
- glycine(out) + n H+(in) = glycine(in) + n H+(out)
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
5 mM | 4-aminobutanoate | |||||
3.5 mM | beta-alanine | |||||
0.8 mM | 4-aminobutanoate | |||||
2.1 mM | chloride | |||||
2.3 mM | chloride | for 4-aminobutanoate uptake |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
38 nmol/min/mg | toward beta-alanine | ||||
41 nmol/min/mg | toward 4-aminobutanoate | ||||
90.6 nmol/min/mg | toward chloride | ||||
7.4 nmol/min/mg | toward chloride (for 4-aminobutanoate uptake) |
GO annotations
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameVesicular inhibitory amino acid transporter
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionO35458
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Multi-pass membrane protein
Note: Presents in glycine-, GABA- or GABA- and glycine-containing boutons.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-132 | Cytoplasmic | ||||
Sequence: MATLLRSKLTNVATSVSNKSQAKVSGMFARMGFQAATDEEAVGFAHCDDLDFEHRQGLQMDILKSEGEPCGDEGAEPPVEGDIHYQRGGAPLPPSGSKDQAVGAGGEFGGHDKPKITAWEAGWNVTNAIQGM | ||||||
Transmembrane | 133-153 | Helical | ||||
Sequence: FVLGLPYAILHGGYLGLFLII | ||||||
Topological domain | 154-204 | Lumenal, vesicle | ||||
Sequence: FAAVVCCYTGKILIACLYEENEDGEVVRVRDSYVAIANACCAPRFPTLGGR | ||||||
Transmembrane | 205-225 | Helical | ||||
Sequence: VVNVAQIIELVMTCILYVVVS | ||||||
Topological domain | 226-265 | Cytoplasmic | ||||
Sequence: GNLMYNSFPGLPVSQKSWSIIATAVLLPCAFLKNLKAVSK | ||||||
Transmembrane | 266-286 | Helical | ||||
Sequence: FSLLCTLAHFVINILVIAYCL | ||||||
Topological domain | 287-305 | Lumenal, vesicle | ||||
Sequence: SRARDWAWEKVKFYIDVKK | ||||||
Transmembrane | 306-326 | Helical | ||||
Sequence: FPISIGIIVFSYTSQIFLPSL | ||||||
Topological domain | 327-341 | Cytoplasmic | ||||
Sequence: EGNMQQPSEFHCMMN | ||||||
Transmembrane | 342-362 | Helical | ||||
Sequence: WTHIAACVLKGLFALVAYLTW | ||||||
Topological domain | 363-383 | Lumenal, vesicle | ||||
Sequence: ADETKEVITDNLPGSIRAVVN | ||||||
Transmembrane | 384-404 | Helical | ||||
Sequence: IFLVAKALLSYPLPFFAAVEV | ||||||
Topological domain | 405-438 | Cytoplasmic | ||||
Sequence: LEKSLFQEGSRAFFPACYGGDGRLKSWGLTLRCA | ||||||
Transmembrane | 439-459 | Helical | ||||
Sequence: LVVFTLLMAIYVPHFALLMGL | ||||||
Topological domain | 460-461 | Lumenal, vesicle | ||||
Sequence: TG | ||||||
Transmembrane | 462-482 | Helical | ||||
Sequence: SLTGAGLCFLLPSLFHLRLLW | ||||||
Topological domain | 483-489 | Cytoplasmic | ||||
Sequence: RKLLWHQ | ||||||
Transmembrane | 490-510 | Helical | ||||
Sequence: VFFDVAIFVIGGICSVSGFVH | ||||||
Topological domain | 511-525 | Lumenal, vesicle | ||||
Sequence: SLEGLIEAYRTNAED |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 213 | Loss of GABA and glycine transport activity. Loss of potential gradient-induced Cl- uptake. Loss of beta-alanine transport activity. | ||||
Sequence: E → A | ||||||
Mutagenesis | 351 | Retains around 80% of the beta-alanine transport activity. | ||||
Sequence: K → A |
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000093823 | 1-525 | Vesicular inhibitory amino acid transporter | |||
Sequence: MATLLRSKLTNVATSVSNKSQAKVSGMFARMGFQAATDEEAVGFAHCDDLDFEHRQGLQMDILKSEGEPCGDEGAEPPVEGDIHYQRGGAPLPPSGSKDQAVGAGGEFGGHDKPKITAWEAGWNVTNAIQGMFVLGLPYAILHGGYLGLFLIIFAAVVCCYTGKILIACLYEENEDGEVVRVRDSYVAIANACCAPRFPTLGGRVVNVAQIIELVMTCILYVVVSGNLMYNSFPGLPVSQKSWSIIATAVLLPCAFLKNLKAVSKFSLLCTLAHFVINILVIAYCLSRARDWAWEKVKFYIDVKKFPISIGIIVFSYTSQIFLPSLEGNMQQPSEFHCMMNWTHIAACVLKGLFALVAYLTWADETKEVITDNLPGSIRAVVNIFLVAKALLSYPLPFFAAVEVLEKSLFQEGSRAFFPACYGGDGRLKSWGLTLRCALVVFTLLMAIYVPHFALLMGLTGSLTGAGLCFLLPSLFHLRLLWRKLLWHQVFFDVAIFVIGGICSVSGFVHSLEGLIEAYRTNAED | ||||||
Modified residue | 186 | 3'-nitrotyrosine | ||||
Sequence: Y |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Brain (PubMed:9349821, PubMed:9822734).
Expressed at high levels within the neocortex, hippocampus, cerebellum, striatum, septal nuclei and the reticular nucleus of the thalamus (PubMed:9349821).
Also expressed in islets where it is more abundant in the peripheral/mantle region (PubMed:12031963).
Highly expressed in the nerve endings of GABA neurons in the brain and spinal cord but also in glycinergic nerve endings (PubMed:9822734).
Expressed in glycine-, GABA- or GABA- and glycine-containing boutons (PubMed:10036231, PubMed:9822734).
Expressed at high levels within the neocortex, hippocampus, cerebellum, striatum, septal nuclei and the reticular nucleus of the thalamus (PubMed:9349821).
Also expressed in islets where it is more abundant in the peripheral/mantle region (PubMed:12031963).
Highly expressed in the nerve endings of GABA neurons in the brain and spinal cord but also in glycinergic nerve endings (PubMed:9822734).
Expressed in glycine-, GABA- or GABA- and glycine-containing boutons (PubMed:10036231, PubMed:9822734).
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Length525
- Mass (Da)57,407
- Last updated1998-01-01 v1
- Checksum33C5E8D31B7BD510
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF030253 EMBL· GenBank· DDBJ | AAB82950.1 EMBL· GenBank· DDBJ | mRNA |