O35440 · MOT2_MERUN
- ProteinMonocarboxylate transporter 2
- GeneSLC16A7
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids484 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Proton-coupled monocarboxylate symporter. Catalyzes the rapid transport across the plasma membrane of monocarboxylates such as L-lactate, pyruvate and ketone bodies, acetoacetate, beta-hydroxybutyrate and acetate. Dimerization is functionally required and both subunits work cooperatively in transporting substrate.
Catalytic activity
- 3-methyl-2-oxobutanoate(out) + H+(out) = 3-methyl-2-oxobutanoate(in) + H+(in)3-methyl-2-oxobutanoate (out)CHEBI:11851
+ H+ (out)CHEBI:15378= CHEBI:11851 + H+ (in)CHEBI:15378 - (S)-lactate(in) + H+(in) = (S)-lactate(out) + H+(out)
- acetoacetate(out) + H+(out) = acetoacetate(in) + H+(in)
- (R)-3-hydroxybutanoate(out) + H+(out) = (R)-3-hydroxybutanoate(in) + H+(in)
- 4-methyl-2-oxopentanoate(out) + H+(out) = 4-methyl-2-oxopentanoate(in) + H+(in)
- H+(out) + pyruvate(out) = H+(in) + pyruvate(in)This reaction proceeds in the forward and the backward directions.
- (S)-3-hydroxybutanoate(out) + H+(out) = (S)-3-hydroxybutanoate(in) + H+(in)
Activity regulation
Transport activity exhibits steep dependence on substrate concentration. Substrate concentration sensitivity of SLC16A7 arises from the strong inter-subunit cooperativity of the SLC16A7 dimer during transport. Inhibited by AR-C155858.
Features
Showing features for site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 292 | May be protonated during monocarboxylate transport | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | basolateral plasma membrane | |
Cellular Component | cytoplasm | |
Cellular Component | plasma membrane | |
Molecular Function | identical protein binding | |
Molecular Function | lactate transmembrane transporter activity | |
Molecular Function | pyruvate transmembrane transporter activity | |
Molecular Function | symporter activity | |
Biological Process | lactate transmembrane transport | |
Biological Process | pyruvate transmembrane transport |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameMonocarboxylate transporter 2
- Short namesMCT 2
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Gerbillinae > Meriones
Accessions
- Primary accessionO35440
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Basolateral cell membrane ; Multi-pass membrane protein
Note: Requires the ancillary protein, EMB for plasma membrane localization (By similarity).
Colocalizes with BSG in spermatozoa. Detected in the cytoplasm of Sertoli cells (By similarity).
Colocalizes with BSG in spermatozoa. Detected in the cytoplasm of Sertoli cells (By similarity).
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-16 | Cytoplasmic | ||||
Sequence: MPAPTAVPPPHPLPPD | ||||||
Transmembrane | 17-37 | Helical; Name=1 | ||||
Sequence: GGWGWVVVGASFISIGFSYAF | ||||||
Topological domain | 38-60 | Extracellular | ||||
Sequence: PKSVTVFFKDIQEIFRAGHSKVA | ||||||
Transmembrane | 61-81 | Helical; Name=2 | ||||
Sequence: WISSIMLAVMYAGGPISSVLV | ||||||
Topological domain | 82-87 | Cytoplasmic | ||||
Sequence: NKYGSR | ||||||
Transmembrane | 88-108 | Helical; Name=3 | ||||
Sequence: PVVVIGGLLCCTGMILASFST | ||||||
Topological domain | 109-116 | Extracellular | ||||
Sequence: SMIQLYLT | ||||||
Transmembrane | 117-137 | Helical; Name=4 | ||||
Sequence: IGFISGLGLAFNLQPALTILG | ||||||
Topological domain | 138-144 | Cytoplasmic | ||||
Sequence: KYFYRRR | ||||||
Transmembrane | 145-165 | Helical; Name=5 | ||||
Sequence: PLASGLAMTGSPVFLSSLAPF | ||||||
Topological domain | 166-174 | Extracellular | ||||
Sequence: NQYLFNSYG | ||||||
Transmembrane | 175-195 | Helical; Name=6 | ||||
Sequence: LKGSFLILGGIFLHSCVAGSL | ||||||
Topological domain | 196-245 | Cytoplasmic | ||||
Sequence: MRPVGTSQQSPKSKSKVSSRHDSSTKKAPKLTLAQRINMFLDFSLFKHRG | ||||||
Transmembrane | 246-266 | Helical; Name=7 | ||||
Sequence: FLIYLSGNVIMFLGFFAPVIF | ||||||
Topological domain | 267-281 | Extracellular | ||||
Sequence: LSPYAKNRGVDDYKA | ||||||
Transmembrane | 282-302 | Helical; Name=8 | ||||
Sequence: AYLLSVMAFVDMFSRPCGGLI | ||||||
Topological domain | 303-311 | Cytoplasmic | ||||
Sequence: ANTRLVRPR | ||||||
Transmembrane | 312-332 | Helical; Name=9 | ||||
Sequence: IQYFFSLAIVFTGVCHLLCPL | ||||||
Topological domain | 333-337 | Extracellular | ||||
Sequence: AESYT | ||||||
Transmembrane | 338-358 | Helical; Name=10 | ||||
Sequence: ALVVYAIFFGYGFGSVSSILF | ||||||
Topological domain | 359-372 | Cytoplasmic | ||||
Sequence: ETLMDLVGPARFSS | ||||||
Transmembrane | 373-393 | Helical; Name=11 | ||||
Sequence: AVGLVTIVECCPVLLGPPLAG | ||||||
Topological domain | 394-405 | Extracellular | ||||
Sequence: KLVDETGEHKYL | ||||||
Transmembrane | 406-426 | Helical; Name=12 | ||||
Sequence: FVASGAIVVLAGIWLFIGNAI | ||||||
Topological domain | 427-484 | Cytoplasmic | ||||
Sequence: NYRLLAKERKREKARKKKSPNRHSKELESLSKSNQDDVAVRVPQAHRSPSDKERESNI |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000211387 | 1-484 | Monocarboxylate transporter 2 | |||
Sequence: MPAPTAVPPPHPLPPDGGWGWVVVGASFISIGFSYAFPKSVTVFFKDIQEIFRAGHSKVAWISSIMLAVMYAGGPISSVLVNKYGSRPVVVIGGLLCCTGMILASFSTSMIQLYLTIGFISGLGLAFNLQPALTILGKYFYRRRPLASGLAMTGSPVFLSSLAPFNQYLFNSYGLKGSFLILGGIFLHSCVAGSLMRPVGTSQQSPKSKSKVSSRHDSSTKKAPKLTLAQRINMFLDFSLFKHRGFLIYLSGNVIMFLGFFAPVIFLSPYAKNRGVDDYKAAYLLSVMAFVDMFSRPCGGLIANTRLVRPRIQYFFSLAIVFTGVCHLLCPLAESYTALVVYAIFFGYGFGSVSSILFETLMDLVGPARFSSAVGLVTIVECCPVLLGPPLAGKLVDETGEHKYLFVASGAIVVLAGIWLFIGNAINYRLLAKERKREKARKKKSPNRHSKELESLSKSNQDDVAVRVPQAHRSPSDKERESNI |
Interaction
Subunit
Homodimer (By similarity).
Interacts with GRID2IP (By similarity).
Interacts with EMB; interaction mediates SLC16A7 targeting to the plasma membrane (By similarity).
Interacts with isoform 2 of BSG (By similarity).
Interacts with GRID2IP (By similarity).
Interacts with EMB; interaction mediates SLC16A7 targeting to the plasma membrane (By similarity).
Interacts with isoform 2 of BSG (By similarity).
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 198-223 | Disordered | ||||
Sequence: PVGTSQQSPKSKSKVSSRHDSSTKKA | ||||||
Region | 437-484 | Disordered | ||||
Sequence: REKARKKKSPNRHSKELESLSKSNQDDVAVRVPQAHRSPSDKERESNI |
Sequence similarities
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length484
- Mass (Da)52,758
- Last updated2022-10-12 v2
- Checksum753469FDF9213AFD
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
NHTI01001178 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AF029767 EMBL· GenBank· DDBJ | AAB84219.1 EMBL· GenBank· DDBJ | mRNA |