O35394 · PRAF1_RAT

  • Protein
    Prenylated Rab acceptor protein 1
  • Gene
    Rabac1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

General Rab protein regulator required for vesicle formation from the Golgi complex. May control vesicle docking and fusion by mediating the action of Rab GTPases to the SNARE complexes. In addition it inhibits the removal of Rab GTPases from the membrane by GDI1.

Caution

In contrast to the mouse ortholog, it does not interact with the Ras-like GTPases RAC1 and RHOA.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentglutamatergic synapse
Cellular ComponentGolgi apparatus
Cellular Componentmembrane
Cellular Componentplasma membrane
Cellular Componentpresynapse
Cellular Componentsynapse
Cellular Componentsynaptic vesicle
Molecular FunctionGTPase binding
Molecular Functionidentical protein binding
Molecular Functionproline-rich region binding
Molecular Functionprotein-macromolecule adaptor activity
Biological Processvesicle-mediated transport

Names & Taxonomy

Protein names

  • Recommended name
    Prenylated Rab acceptor protein 1
  • Alternative names
    • PRA1 family protein 1

Gene names

    • Name
      Rabac1
    • Synonyms
      Pra1, Praf1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    O35394

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Multi-pass membrane protein
Cytoplasm
Golgi apparatus
Note: According to some authors, it is an integral membrane protein, while others showed that it is cytoplasmic and membrane-associated to Golgi (PubMed:10751420, PubMed:12107180, PubMed:11096102) and synaptic vesicles (PubMed:10707984).

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-78Cytoplasmic
Transmembrane79-94Helical
Transmembrane95-112Helical
Topological domain113-131Cytoplasmic
Transmembrane132-148Helical
Transmembrane149-165Helical
Topological domain166-185Cytoplasmic

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis70Retained in endoplasmic reticulum, no interaction with RAB3A or VAMP2.
Mutagenesis73Retained in endoplasmic reticulum, Golgi and tubular structures, no interaction with RAB3A.
Mutagenesis76Increased interaction with RAB3A or VAMP2, Golgi condensation.
Mutagenesis77No effect.
Mutagenesis78Retained in endoplasmic reticulum, no interaction with RAB3A or VAMP2.
Mutagenesis154No effect.
Mutagenesis161Increased interaction with RAB3A or VAMP2, Golgi condensation.
Mutagenesis166Retained in endoplasmic reticulum, Golgi and tubular structures, no interaction with RAB3A.
Mutagenesis176Retained in endoplasmic reticulum.
Mutagenesis178Retained in endoplasmic reticulum.
Mutagenesis179Retained in endoplasmic reticulum.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002208801-185Prenylated Rab acceptor protein 1

Proteomic databases

PTM databases

Expression

Tissue specificity

Ubiquitous.

Gene expression databases

Interaction

Subunit

Homodimers (By similarity).
Interacts specifically with both prenylated Rab proteins (including RAB3A and RAB1), and VAMP2 (synaptobrevin-2), in an exclusive way. Interacts with NDRG1 (By similarity).
Interacts with free GDI1 in the absence of Rab proteins. Also interacts with PCLO

Protein-protein interaction databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region30-54Required for interaction with prenylated RAB3A and VAMP2
Region165-185Required for interaction with GDI1
Region175-185Homodimerization
Region175-185Required for interaction with prenylated RAB3A and VAMP2

Sequence similarities

Belongs to the PRA1 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    185
  • Mass (Da)
    20,643
  • Last updated
    1998-01-01 v1
  • Checksum
    DA6341AE66F5C2F0
MAAQKDQQKDAEVEGLSATTLLPKLIPSGAGREWLERRRATIRPWGTFVDQQRFSRPRNVGELCQRLVRNVEYYQSNYVFVFLGLILYCVVTSPMLLVALAVFFGACYILYLRTLQSKLVLFGREVSPAHQYALAGGVSFPFFWLAGAGSAVFWVLGATLVLIGSHAAFHQIEPADGEELQMEPV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF025506
EMBL· GenBank· DDBJ
AAB81721.1
EMBL· GenBank· DDBJ
mRNA
BC086387
EMBL· GenBank· DDBJ
AAH86387.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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