O35394 · PRAF1_RAT
- ProteinPrenylated Rab acceptor protein 1
- GeneRabac1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids185 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
General Rab protein regulator required for vesicle formation from the Golgi complex. May control vesicle docking and fusion by mediating the action of Rab GTPases to the SNARE complexes. In addition it inhibits the removal of Rab GTPases from the membrane by GDI1.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | glutamatergic synapse | |
Cellular Component | Golgi apparatus | |
Cellular Component | membrane | |
Cellular Component | plasma membrane | |
Cellular Component | presynapse | |
Cellular Component | synapse | |
Cellular Component | synaptic vesicle | |
Molecular Function | GTPase binding | |
Molecular Function | identical protein binding | |
Molecular Function | proline-rich region binding | |
Molecular Function | protein-macromolecule adaptor activity | |
Biological Process | vesicle-mediated transport |
Names & Taxonomy
Protein names
- Recommended namePrenylated Rab acceptor protein 1
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionO35394
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-78 | Cytoplasmic | ||||
Sequence: MAAQKDQQKDAEVEGLSATTLLPKLIPSGAGREWLERRRATIRPWGTFVDQQRFSRPRNVGELCQRLVRNVEYYQSNY | ||||||
Transmembrane | 79-94 | Helical | ||||
Sequence: VFVFLGLILYCVVTSP | ||||||
Transmembrane | 95-112 | Helical | ||||
Sequence: MLLVALAVFFGACYILYL | ||||||
Topological domain | 113-131 | Cytoplasmic | ||||
Sequence: RTLQSKLVLFGREVSPAHQ | ||||||
Transmembrane | 132-148 | Helical | ||||
Sequence: YALAGGVSFPFFWLAGA | ||||||
Transmembrane | 149-165 | Helical | ||||
Sequence: GSAVFWVLGATLVLIGS | ||||||
Topological domain | 166-185 | Cytoplasmic | ||||
Sequence: HAAFHQIEPADGEELQMEPV |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 70 | Retained in endoplasmic reticulum, no interaction with RAB3A or VAMP2. | ||||
Sequence: N → T | ||||||
Mutagenesis | 73 | Retained in endoplasmic reticulum, Golgi and tubular structures, no interaction with RAB3A. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 76 | Increased interaction with RAB3A or VAMP2, Golgi condensation. | ||||
Sequence: S → A or V | ||||||
Mutagenesis | 77 | No effect. | ||||
Sequence: N → A | ||||||
Mutagenesis | 78 | Retained in endoplasmic reticulum, no interaction with RAB3A or VAMP2. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 154 | No effect. | ||||
Sequence: W → A | ||||||
Mutagenesis | 161 | Increased interaction with RAB3A or VAMP2, Golgi condensation. | ||||
Sequence: V → A | ||||||
Mutagenesis | 166 | Retained in endoplasmic reticulum, Golgi and tubular structures, no interaction with RAB3A. | ||||
Sequence: H → A | ||||||
Mutagenesis | 176 | Retained in endoplasmic reticulum. | ||||
Sequence: D → A | ||||||
Mutagenesis | 178 | Retained in endoplasmic reticulum. | ||||
Sequence: E → A | ||||||
Mutagenesis | 179 | Retained in endoplasmic reticulum. | ||||
Sequence: E → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000220880 | 1-185 | Prenylated Rab acceptor protein 1 | |||
Sequence: MAAQKDQQKDAEVEGLSATTLLPKLIPSGAGREWLERRRATIRPWGTFVDQQRFSRPRNVGELCQRLVRNVEYYQSNYVFVFLGLILYCVVTSPMLLVALAVFFGACYILYLRTLQSKLVLFGREVSPAHQYALAGGVSFPFFWLAGAGSAVFWVLGATLVLIGSHAAFHQIEPADGEELQMEPV |
Proteomic databases
PTM databases
Expression
Interaction
Subunit
Homodimers (By similarity).
Interacts specifically with both prenylated Rab proteins (including RAB3A and RAB1), and VAMP2 (synaptobrevin-2), in an exclusive way. Interacts with NDRG1 (By similarity).
Interacts with free GDI1 in the absence of Rab proteins. Also interacts with PCLO
Interacts specifically with both prenylated Rab proteins (including RAB3A and RAB1), and VAMP2 (synaptobrevin-2), in an exclusive way. Interacts with NDRG1 (By similarity).
Interacts with free GDI1 in the absence of Rab proteins. Also interacts with PCLO
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 30-54 | Required for interaction with prenylated RAB3A and VAMP2 | ||||
Sequence: AGREWLERRRATIRPWGTFVDQQRF | ||||||
Region | 165-185 | Required for interaction with GDI1 | ||||
Sequence: SHAAFHQIEPADGEELQMEPV | ||||||
Region | 175-185 | Homodimerization | ||||
Sequence: ADGEELQMEPV | ||||||
Region | 175-185 | Required for interaction with prenylated RAB3A and VAMP2 | ||||
Sequence: ADGEELQMEPV |
Sequence similarities
Belongs to the PRA1 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length185
- Mass (Da)20,643
- Last updated1998-01-01 v1
- ChecksumDA6341AE66F5C2F0
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF025506 EMBL· GenBank· DDBJ | AAB81721.1 EMBL· GenBank· DDBJ | mRNA | ||
BC086387 EMBL· GenBank· DDBJ | AAH86387.1 EMBL· GenBank· DDBJ | mRNA |