O35308 · MOT3_MOUSE
- ProteinMonocarboxylate transporter 3
- GeneSlc16a8
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids492 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Probable retinal pigment epithelium (RPE)-specific proton-coupled L-lactate transporter (By similarity).
May facilitate transport of lactate and H+ out of the retina and could therefore play an essential role in maintenance of metabolic and ionic homeostasis of the outer retina (PubMed:18524945).
May facilitate transport of lactate and H+ out of the retina and could therefore play an essential role in maintenance of metabolic and ionic homeostasis of the outer retina (PubMed:18524945).
Catalytic activity
- (S)-lactate(in) + H+(in) = (S)-lactate(out) + H+(out)(S)-lactate (in)CHEBI:16651
+ H+ (in)CHEBI:15378= (S)-lactate (out)CHEBI:16651+ H+ (out)CHEBI:15378
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | apical plasma membrane | |
Cellular Component | basal plasma membrane | |
Cellular Component | basolateral plasma membrane | |
Cellular Component | plasma membrane | |
Molecular Function | lactate transmembrane transporter activity | |
Molecular Function | monocarboxylic acid transmembrane transporter activity | |
Molecular Function | symporter activity | |
Biological Process | monocarboxylic acid transport |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMonocarboxylate transporter 3
- Short namesMCT 3
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionO35308
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Basolateral cell membrane ; Multi-pass membrane protein
Note: Basolateral sorting signals (BLSS) in C-terminal cytoplasmic tail ensure its basolateral expression (By similarity).
Colocalizes with BSG in basolateral cell membrane of the retinal pigment epithelium (PubMed:18524945).
Colocalizes with BSG in basolateral cell membrane of the retinal pigment epithelium (PubMed:18524945).
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-14 | Cytoplasmic | ||||
Sequence: MGAGGPRRGAGPPD | ||||||
Transmembrane | 15-35 | Helical | ||||
Sequence: GGWGWVVLGACFVVTGFAYGF | ||||||
Topological domain | 36-58 | Extracellular | ||||
Sequence: PKAVSVFFRELKRDFGAGYSDTA | ||||||
Transmembrane | 59-79 | Helical | ||||
Sequence: WVSSIMLAMLYGTGPLSSILV | ||||||
Topological domain | 80-85 | Cytoplasmic | ||||
Sequence: TRFGCR | ||||||
Transmembrane | 86-106 | Helical | ||||
Sequence: PVMLAGGLLASAGMILASFAS | ||||||
Topological domain | 107-115 | Extracellular | ||||
Sequence: RLVELYLTA | ||||||
Transmembrane | 116-136 | Helical | ||||
Sequence: GVLTGLGLALNFQPSLIMLGL | ||||||
Topological domain | 137-146 | Cytoplasmic | ||||
Sequence: YFERRRPLAN | ||||||
Transmembrane | 147-167 | Helical | ||||
Sequence: GLAAAGSPVFLSMLSPLGQLL | ||||||
Topological domain | 168-172 | Extracellular | ||||
Sequence: GERFG | ||||||
Transmembrane | 173-193 | Helical | ||||
Sequence: WRGGFLLFGGLLLHCCACGAV | ||||||
Topological domain | 194-228 | Cytoplasmic | ||||
Sequence: MRPPPGPPPRRDPSPHGGPARRRRLLDVAVCTDRA | ||||||
Transmembrane | 229-249 | Helical | ||||
Sequence: FVVYVVTKFLMALGLFVPAIL | ||||||
Topological domain | 250-257 | Extracellular | ||||
Sequence: LVNYAKDA | ||||||
Transmembrane | 258-278 | Helical | ||||
Sequence: GVPDAEAAFLLSIVGFVDIVA | ||||||
Topological domain | 279-293 | Cytoplasmic | ||||
Sequence: RPACGALAGLGRLRP | ||||||
Transmembrane | 294-314 | Helical | ||||
Sequence: HVPYLFSLALLANGLTDLISA | ||||||
Topological domain | 315-318 | Extracellular | ||||
Sequence: RARS | ||||||
Transmembrane | 319-339 | Helical | ||||
Sequence: YGTLVAFCIAFGLSYGMVGAL | ||||||
Topological domain | 340-352 | Cytoplasmic | ||||
Sequence: QFEVLMATVGAPR | ||||||
Transmembrane | 353-373 | Helical | ||||
Sequence: FPSALGLVLLVEAVAVLIGPP | ||||||
Topological domain | 374-386 | Extracellular | ||||
Sequence: SAGRLVDALKNYE | ||||||
Transmembrane | 387-407 | Helical | ||||
Sequence: IIFYLAGSEVALAGVFMAVTT | ||||||
Topological domain | 408-492 | Cytoplasmic | ||||
Sequence: YCCLRCSKNISSGRSAEGGASDPEDVEAERDSEPMPASTEEPGSLEALEVLSPRAGSPEQEPEEEAVPELDHESIGGHEARGQKA |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Deficient mice exhibit significant differences in lactate concentration and pH in the retina and display reduced visual function.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 28 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000211391 | 1-492 | Monocarboxylate transporter 3 | |||
Sequence: MGAGGPRRGAGPPDGGWGWVVLGACFVVTGFAYGFPKAVSVFFRELKRDFGAGYSDTAWVSSIMLAMLYGTGPLSSILVTRFGCRPVMLAGGLLASAGMILASFASRLVELYLTAGVLTGLGLALNFQPSLIMLGLYFERRRPLANGLAAAGSPVFLSMLSPLGQLLGERFGWRGGFLLFGGLLLHCCACGAVMRPPPGPPPRRDPSPHGGPARRRRLLDVAVCTDRAFVVYVVTKFLMALGLFVPAILLVNYAKDAGVPDAEAAFLLSIVGFVDIVARPACGALAGLGRLRPHVPYLFSLALLANGLTDLISARARSYGTLVAFCIAFGLSYGMVGALQFEVLMATVGAPRFPSALGLVLLVEAVAVLIGPPSAGRLVDALKNYEIIFYLAGSEVALAGVFMAVTTYCCLRCSKNISSGRSAEGGASDPEDVEAERDSEPMPASTEEPGSLEALEVLSPRAGSPEQEPEEEAVPELDHESIGGHEARGQKA |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed exclusively in retinal pigment epithelium and choroid plexus epithelium.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 419-492 | Disordered | ||||
Sequence: SGRSAEGGASDPEDVEAERDSEPMPASTEEPGSLEALEVLSPRAGSPEQEPEEEAVPELDHESIGGHEARGQKA | ||||||
Region | 426-460 | Basolateral sorting signal | ||||
Sequence: GASDPEDVEAERDSEPMPASTEEPGSLEALEVLSP | ||||||
Compositional bias | 427-443 | Basic and acidic residues | ||||
Sequence: ASDPEDVEAERDSEPMP | ||||||
Region | 461-482 | Basolateral sorting signal | ||||
Sequence: RAGSPEQEPEEEAVPELDHESI | ||||||
Compositional bias | 476-492 | Basic and acidic residues | ||||
Sequence: ELDHESIGGHEARGQKA |
Domain
The two basolateral sorting signals (BLSS) in C-terminal cytoplasmic tails direct SLC16A8 to the basolateral membrane.
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length492
- Mass (Da)51,556
- Last updated2000-10-01 v2
- Checksum2B19E362B96D9ACC
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 427-443 | Basic and acidic residues | ||||
Sequence: ASDPEDVEAERDSEPMP | ||||||
Compositional bias | 476-492 | Basic and acidic residues | ||||
Sequence: ELDHESIGGHEARGQKA |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF019111 EMBL· GenBank· DDBJ | AAB70582.2 EMBL· GenBank· DDBJ | mRNA | ||
AF178956 EMBL· GenBank· DDBJ | AAF45042.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC018216 EMBL· GenBank· DDBJ | AAH18216.1 EMBL· GenBank· DDBJ | mRNA |