O35240 · ASIC3_RAT

  • Protein
    Acid-sensing ion channel 3
  • Gene
    Asic3
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties.

Miscellaneous

Potentiated by FMRFamide-related neuropeptides (By similarity).
Sensitized and potentiated by NPFF and NPSF. Regulated by lactate and Ca2+. Specifically inhibited by APETx2, a sea anemone toxin. Inhibited by anti-inflammatory drugs like salicylic acid.

Features

Showing features for site.

153350100150200250300350400450500
TypeIDPosition(s)Description
Site26Potassium ion selectivity and permeability

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentperinuclear region of cytoplasm
Cellular Componentplasma membrane
Molecular Functionenterobactin transmembrane transporter activity
Molecular Functionligand-gated sodium channel activity
Molecular Functionmonoatomic cation channel activity
Molecular FunctionpH-gated monoatomic ion channel activity
Biological Processdetection of chemical stimulus involved in sensory perception
Biological Processdetection of chemical stimulus involved in sensory perception of pain
Biological Processdetection of mechanical stimulus involved in sensory perception
Biological Processdetection of mechanical stimulus involved in sensory perception of pain
Biological Processdetection of temperature stimulus involved in sensory perception
Biological Processdetection of temperature stimulus involved in sensory perception of pain
Biological Processestablishment of localization in cell
Biological Processmonoatomic cation transport
Biological Processmonoatomic ion transmembrane transport
Biological Processresponse to acidic pH
Biological Processresponse to heat
Biological Processresponse to mechanical stimulus
Biological Processsensory perception of sour taste
Biological Processsodium ion transmembrane transport
Biological Processsodium ion transport

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Acid-sensing ion channel 3
  • Short names
    ASIC3
  • Alternative names
    • Amiloride-sensitive cation channel 3
    • Dorsal root ASIC (DRASIC)

Gene names

    • Name
      Asic3
    • Synonyms
      Accn3, Drasic

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    O35240

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Cell membrane
; Multi-pass membrane protein
Note: In part cytoplasmic in cochlea cells (By similarity).
Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-19Cytoplasmic
Transmembrane20-40Helical
Topological domain41-435Extracellular
Transmembrane436-456Helical
Topological domain457-533Cytoplasmic

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis20No effect on selectivity or channel function.
Mutagenesis21Loss of channel function.
Mutagenesis26Alters selectivity of the channel for sodium. No effect on channel function.
Mutagenesis40Loss of regulation by PKC through PRKCABP; when associated with G-523.
Mutagenesis523Loss of regulation by PKC through PRKCABP; when associated with G-40.

Chemistry

PTM/Processing

Features

Showing features for chain, modified residue, disulfide bond, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00001813031-533Acid-sensing ion channel 3
Modified residue40Phosphothreonine; by PKC
Disulfide bond93↔187
Disulfide bond165↔172
Glycosylation176N-linked (GlcNAc...) asparagine
Disulfide bond283↔372
Disulfide bond317↔368
Disulfide bond321↔366
Disulfide bond330↔352
Disulfide bond332↔344
Glycosylation400N-linked (GlcNAc...) asparagine
Modified residue523Phosphoserine; by PKC

Post-translational modification

Phosphorylated by PKA (By similarity).
Phosphorylated by PKC. In vitro, PRKCABP/PICK-1 is necessary for PKC phosphorylation and activation of a ASIC3/ACCN3-ASIC2/ASIC2b channel, but does not activate a homomeric ASIC3/ACCN3 channel

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in sciatic nerve and dorsal root ganglion (at protein level). Expressed in sensory neurons of dorsal root ganglion. Expressed in Golgi interneurons in the granular layer. Also found in superior cervical ganglia, spinal cord and brain stem.

Induction

Transcriptionally regulated by the proinflammatory mediators nerve growth factor, serotonin, interleukin-1 and bradykinin. Up-regulation upon tissue inflammation is abolished by anti-inflammatory drugs.

Developmental stage

Expression is first detected at 15.5 dpc. Strongly expressed perinatally.

Gene expression databases

Interaction

Subunit

Homotrimer or heterotrimer with other ASIC proteins (By similarity).
Interacts with LIN7B, MAGI1 and GOPC (By similarity).
Interacts with DLG4 and ASIC2. Interacts with STOM; this regulates channel activity. Homotrimeric ASIC3 and ASIC3-containing heterotrimers interact with the cono-RFamide CNF-Tx1.1, and probably CNF-Tx1.2 and CNF-Tx1.3 (AC P0DL71) (PubMed:28396446).

Binary interactions

Protein-protein interaction databases

Chemistry

Structure

Family & Domains

Features

Showing features for region, motif.

TypeIDPosition(s)Description
Region286-310Disordered
Motif530-533PDZ-binding

Domain

The PDZ domain-binding motif is involved in interaction with LIN7A, GOPC and MAGI1/BAIAP1.

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    533
  • Mass (Da)
    59,227
  • Last updated
    1998-01-01 v1
  • Checksum
    294B57322C74B3DC
MKPRSGLEEAQRRQASDIRVFASSCTMHGLGHIFGPGGLTLRRGLWATAVLLSLAAFLYQVAERVRYYGEFHHKTTLDERESHQLTFPAVTLCNINPLRRSRLTPNDLHWAGTALLGLDPAEHAAYLRALGQPPAPPGFMPSPTFDMAQLYARAGHSLEDMLLDCRYRGQPCGPENFTVIFTRMGQCYTFNSGAHGAELLTTPKGGAGNGLEIMLDVQQEEYLPIWKDMEETPFEVGIRVQIHSQDEPPAIDQLGFGAAPGHQTFVSCQQQQLSFLPPPWGDCNTASLDPDDFDPEPSDPLGSPRPRPSPPYSLIGCRLACESRYVARKCGCRMMHMPGNSPVCSPQQYKDCASPALDAMLRKDTCVCPNPCATTRYAKELSMVRIPSRASARYLARKYNRSESYITENVLVLDIFFEALNYEAVEQKAAYEVSELLGDIGGQMGLFIGASLLTILEILDYLCEVFQDRVLGYFWNRRSAQKRSGNTLLQEELNGHRTHVPHLSLGPRPPTTPCAVTKTLSASHRTCYLVTRL

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8I5ZRU8A0A8I5ZRU8_RATAsic3443

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF013598
EMBL· GenBank· DDBJ
AAB69328.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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