O34928 · PDAA_BACSU

  • Protein
    Peptidoglycan-N-acetylmuramic acid deacetylase PdaA
  • Gene
    pdaA
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Catalyzes the deacetylation of N-acetylmuramic acid (MurNAc) residues in glycan strands of peptidoglycan, leading to the formation of muramic delta-lactam residues in spore cortex, after transpeptidation of deacetylated muramic acid residues. PdaA probably carries out both deacetylation and lactam ring formation and requires the product of CwlD activity on peptidoglycan as a substrate. Is required for germination. Cannot use chitin oligomer (hexa-N-acetylchitohexaose) as a substrate.

Miscellaneous

CwlD and PdaA are necessary and sufficient for muramic delta-lactam production in B.subtilis spore peptidoglycan.

pH Dependence

Optimum pH is 7.0.

Features

Showing features for active site, binding site, site.

126320406080100120140160180200220240260
TypeIDPosition(s)Description
Active site73Proton acceptor
Binding site124a divalent metal cation (UniProtKB | ChEBI)
Binding site128a divalent metal cation (UniProtKB | ChEBI)
Site193Raises pKa of active site His
Active site222Proton donor

GO annotations

AspectTerm
Molecular Functiondeacetylase activity
Molecular Functionhydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
Molecular Functionmetal ion binding
Biological Processcarbohydrate metabolic process
Biological Processcell wall organization
Biological Processsporulation resulting in formation of a cellular spore

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Peptidoglycan-N-acetylmuramic acid deacetylase PdaA
  • EC number
  • Short names
    Peptidoglycan MurNAc deacetylase

Gene names

    • Name
      pdaA
    • Synonyms
      yfjS
    • Ordered locus names
      BSU07980

Organism names

Accessions

  • Primary accession
    O34928

Proteomes

Phenotypes & Variants

Disruption phenotype

Spores have no muramic delta-lactam structure in the cortex and cannot germinate. Mutant spore peptidoglycan possesses many MurNAc residues lacking peptide side chains.

Chemistry

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-23
ChainPRO_000002484224-263Peptidoglycan-N-acetylmuramic acid deacetylase PdaA

Proteomic databases

Expression

Induction

Expression is sigma G-dependent.

Developmental stage

Expressed in the forespore, then exported into the developing cortex.

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain66-247NodB homology

Sequence similarities

Belongs to the polysaccharide deacetylase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    263
  • Mass (Da)
    30,069
  • Last updated
    1998-01-01 v1
  • Checksum
    FEA2251646D169A0
MKWMCSICCAAVLLAGGAAQAEAVPNEPINWGFKRSVNHQPPDAGKQLNSLIEKYDAFYLGNTKEKTIYLTFDNGYENGYTPKVLDVLKKHRVTGTFFVTGHFVKDQPQLIKRMSDEGHIIGNHSFHHPDLTTKTADQIQDELDSVNEEVYKITGKQDNLYLRPPRGVFSEYVLKETKRLGYQTVFWSVAFVDWKINNQKGKKYAYDHMIKQAHPGAIYLLHTVSRDNAEALDDAITDLKKQGYTFKSIDDLMFEKEMRLPSL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D83967
EMBL· GenBank· DDBJ
BAA23389.1
EMBL· GenBank· DDBJ
Genomic DNA
AL009126
EMBL· GenBank· DDBJ
CAB12627.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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