O34928 · PDAA_BACSU
- ProteinPeptidoglycan-N-acetylmuramic acid deacetylase PdaA
- GenepdaA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids263 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the deacetylation of N-acetylmuramic acid (MurNAc) residues in glycan strands of peptidoglycan, leading to the formation of muramic delta-lactam residues in spore cortex, after transpeptidation of deacetylated muramic acid residues. PdaA probably carries out both deacetylation and lactam ring formation and requires the product of CwlD activity on peptidoglycan as a substrate. Is required for germination. Cannot use chitin oligomer (hexa-N-acetylchitohexaose) as a substrate.
Miscellaneous
CwlD and PdaA are necessary and sufficient for muramic delta-lactam production in B.subtilis spore peptidoglycan.
pH Dependence
Optimum pH is 7.0.
Features
Showing features for active site, binding site, site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | deacetylase activity | |
Molecular Function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds | |
Molecular Function | metal ion binding | |
Biological Process | carbohydrate metabolic process | |
Biological Process | cell wall organization | |
Biological Process | sporulation resulting in formation of a cellular spore |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePeptidoglycan-N-acetylmuramic acid deacetylase PdaA
- EC number
- Short namesPeptidoglycan MurNAc deacetylase
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus
Accessions
- Primary accessionO34928
Proteomes
Phenotypes & Variants
Disruption phenotype
Spores have no muramic delta-lactam structure in the cortex and cannot germinate. Mutant spore peptidoglycan possesses many MurNAc residues lacking peptide side chains.
Chemistry
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-23 | |||||
Sequence: MKWMCSICCAAVLLAGGAAQAEA | ||||||
Chain | PRO_0000024842 | 24-263 | Peptidoglycan-N-acetylmuramic acid deacetylase PdaA | |||
Sequence: VPNEPINWGFKRSVNHQPPDAGKQLNSLIEKYDAFYLGNTKEKTIYLTFDNGYENGYTPKVLDVLKKHRVTGTFFVTGHFVKDQPQLIKRMSDEGHIIGNHSFHHPDLTTKTADQIQDELDSVNEEVYKITGKQDNLYLRPPRGVFSEYVLKETKRLGYQTVFWSVAFVDWKINNQKGKKYAYDHMIKQAHPGAIYLLHTVSRDNAEALDDAITDLKKQGYTFKSIDDLMFEKEMRLPSL |
Proteomic databases
Expression
Induction
Expression is sigma G-dependent.
Developmental stage
Expressed in the forespore, then exported into the developing cortex.
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 66-247 | NodB homology | ||||
Sequence: KTIYLTFDNGYENGYTPKVLDVLKKHRVTGTFFVTGHFVKDQPQLIKRMSDEGHIIGNHSFHHPDLTTKTADQIQDELDSVNEEVYKITGKQDNLYLRPPRGVFSEYVLKETKRLGYQTVFWSVAFVDWKINNQKGKKYAYDHMIKQAHPGAIYLLHTVSRDNAEALDDAITDLKKQGYTFK |
Sequence similarities
Belongs to the polysaccharide deacetylase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length263
- Mass (Da)30,069
- Last updated1998-01-01 v1
- ChecksumFEA2251646D169A0
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D83967 EMBL· GenBank· DDBJ | BAA23389.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL009126 EMBL· GenBank· DDBJ | CAB12627.1 EMBL· GenBank· DDBJ | Genomic DNA |