O34680 · YDIP_BACSU

Function

function

A methylase, recognizes the double-stranded sequence 5'-YTCGAR-3', methylates C-3 on both strands, and protects the DNA from cleavage by the BsuMI endonuclease.

Caution

The characterized enzyme was reported to be a monomer of approximately 45 kDa; it is not clear whether this corresponds to YdiO, YdiP or to another activity altogether.

Catalytic activity

Activity regulation

Somewhat inhibited by MgCl2 and spermidine, strongly inhibited by MnCl2.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.6 μMS-adenosyl-L-methionine
3 nMDNA

pH Dependence

Optimum pH is 8.0.

Temperature Dependence

Rapidly loses activity at 37 degrees Celsius in the absence of DNA.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site69

GO annotations

AspectTerm
Molecular FunctionDNA (cytosine-5-)-methyltransferase activity
Molecular Functionsite-specific DNA-methyltransferase (adenine-specific) activity
Biological ProcessDNA restriction-modification system
Biological Processmethylation

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Type II methyltransferase M2.BsuMI
  • EC number
  • Short names
    M2.BsuMI
  • Alternative names
    • BsuMI modification methylase subunit YdiP
    • Cytosine-specific methyltransferase M2.BsuMI

Gene names

    • Name
      ydiP
    • Ordered locus names
      BSU06070

Organism names

  • Taxonomic identifier
  • Strains
    • 168
    • 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / NRRL NRS-744 / VKM B-501
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus

Accessions

  • Primary accession
    O34680
  • Secondary accessions
    • Q797D0

Proteomes

Phenotypes & Variants

Disruption phenotype

Essential for growth, it can be disrupted once one of the components of the corresponding BsuMI restriction endonuclease complex (AC O34303, O34885, O35025, YdjA, YdiS and YdiR respectively) has been disrupted (PubMed:11751814).
Triple deletions ydiO-ydiP-ydiR, ydiO-ydiP-ydiS and ydiO-ydiP-ydjA lead to loss of susceptibility to MspJI, which only digests C-methylated DNA (PubMed:32324221).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003798851-389Type II methyltransferase M2.BsuMI

Proteomic databases

Expression

Induction

Constitutively expressed during exponential growth. Encoded in an operon with ydiO and in a second with groES, groEL, ydiM and ydiN. This second operon is heat-inducible.

Developmental stage

Not expressed during sporulation.

Interaction

Subunit

Monomer (PubMed:3150363).
May form a complex with YdiP, also seems to be active alone (Probable)

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-299SAM-dependent MTase C5-type

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    389
  • Mass (Da)
    43,284
  • Last updated
    1998-01-01 v1
  • Checksum
    16578833225AFE22
MKVVSLFSGIGGIELGLHQSGHTTEIFCEVDPLAKAVLSKNFPGVKIEDDINEIRELPSCDLVAAGFPCQDLSQAGGKEGIDGSRSGLVKKLFELIEKKEHANRPPWILIENVPYMLRLNRGKAMSYLTSVLSELGYTWAYRTVDARCFGLPQRRHRVILLASLFEDPKDVIFSQDHSEPDLDGKPSVVDHSNYYGFYWTEGLRGVGWAREAVPPIKCGSSVGIASPPAVWSPYEDIVGTINIRDAERLQGFPEDWTNITTETGKDIKEGARWRLVGNAVSVRVSKWIGENLSQPKGSISDFEGELVTKTWPSAAWGYGDKKYKVPVSKWVANTEQIAISEFLNHPLKPLSARALNGFLGRAARCTNVNYSDEFINSLERCKDRQLQKV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB007637
EMBL· GenBank· DDBJ
BAA22751.1
EMBL· GenBank· DDBJ
Genomic DNA
AL009126
EMBL· GenBank· DDBJ
CAB12426.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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