O34667 · LUXS_BACSU
- ProteinS-ribosylhomocysteine lyase
- GeneluxS
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids157 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD).
Catalytic activity
- S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = (S)-4,5-dihydroxypentane-2,3-dione + L-homocysteine
Cofactor
Note: Binds 1 Fe cation per subunit.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | iron ion binding | |
Molecular Function | S-ribosylhomocysteine lyase activity | |
Biological Process | quorum sensing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameS-ribosylhomocysteine lyase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus
Accessions
- Primary accessionO34667
Proteomes
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 57 | Complete loss of activity. | ||||
Sequence: E → A or Q | ||||||
Mutagenesis | 57 | 220-fold decrease in activity. | ||||
Sequence: E → D | ||||||
Mutagenesis | 84 | Complete loss of activity. | ||||
Sequence: C → A | ||||||
Mutagenesis | 84 | Almost complete loss of activity. | ||||
Sequence: C → D or S |
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000172211 | 1-157 | S-ribosylhomocysteine lyase | |||
Sequence: MPSVESFELDHNAVVAPYVRHCGVHKVGTDGVVNKFDIRFCQPNKQAMKPDTIHTLEHLLAFTIRSHAEKYDHFDIIDISPMGCQTGYYLVVSGEPTSAEIVDLLEDTMKEAVEITEIPAANEKQCGQAKLHDLEGAKRLMRFWLSQDKEELLKVFG |
Proteomic databases
Structure
Sequence
- Sequence statusComplete
- Length157
- Mass (Da)17,714
- Last updated1998-01-01 v1
- ChecksumDD011A2A88BD2FFB
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF008220 EMBL· GenBank· DDBJ | AAC00235.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL009126 EMBL· GenBank· DDBJ | CAB15045.1 EMBL· GenBank· DDBJ | Genomic DNA |