O32799 · PFL_LACLM

Function

Miscellaneous

Several mechanisms have been proposed based on complexes formed with substrate analogs. After activation by the glycine radical, the cysteine radical, Cys-417, can abstract hydrogen atoms from the other active site cysteine, Cys-416, and from coenzyme A, and it can also transfer hydrogen atoms to product radicals. The other active site cysteine can attack the central carbonyl of pyruvate and covalently bind the product acetyl group (By similarity).

Catalytic activity

Pathway

Fermentation; pyruvate fermentation; formate from pyruvate: step 1/1.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site416S-acetylcysteine intermediate
Active site417Cysteine radical intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionformate C-acetyltransferase activity
Biological Processglucose metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Formate acetyltransferase
  • EC number
  • Alternative names
    • Pyruvate formate-lyase

Gene names

    • Name
      pfl
    • Ordered locus names
      llmg_0629

Organism names

Accessions

  • Primary accession
    O32799
  • Secondary accessions
    • A2RIY3

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00001666941-787Formate acetyltransferase
Modified residue749Glycine radical

Keywords

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain8-629PFL
Domain645-774Glycine radical

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    787
  • Mass (Da)
    89,107
  • Last updated
    1998-01-01 v1
  • Checksum
    66537F086BC82E7E
MKTEVTENIFEQAWDGFKGTNWRDKASVTRFVQENYKPYDGDESFLAGPTERTLKVKKIIEDTKNHYEEVGFPFDTDRVTSIDKIPAGYIDANDKELELIYGMQNSELFRLNFMPRGGLRVAEKILTEHGLSVDPGLHDVLSQTMTSVNDGIFRAYTSAIRKARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMKEKAKEWDAITEINDDNIRLKEEINMQYQALQEVVNFGALYGLDVSRPAMNVKEAIQWVNIAYMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTEQEIQEFVDDFILKLRTMKFARAAAYDELYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDTIGNAPEPNLTVLWDSKLPYSFKRYSMSMSHKHSSIQYEGVETMAKDGYGEMSCISCCVSPLDPENEEGRHNLQYFGARVNVLKAMLTGLNGGYDDVHKDYKVFDIEPVRDEILDYDTVMENFDKSLNWLTDTYVDAMNIIHYMTDKYNYEAVQMAFLPTKVRANMGFGICGFANTVDSLSAIKYAKVKTLRDENGYIYDYEVEGDFPRYGEDDDRADDIAKLVMKMYHEKLASHKLYKNAEATVSLLTITSNVAYSKQTGNSPVHKGVFLNEDGTVNKSKLEFFSPGANPSNKAKGGWLQNLRSLAKLEFKDANDGISLTTQVSPRALGKTRDEQVDNLVQILDGYFTPGALINGTEFAGQHVNLNVMDLKDVYDKIMRGEDVIVRISGYCVNTKYLTPEQKQELTERVFHEVLSNDDEEVMHTSNI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AJ000325
EMBL· GenBank· DDBJ
CAA03991.1
EMBL· GenBank· DDBJ
Genomic DNA
AM406671
EMBL· GenBank· DDBJ
CAL97231.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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