O32224 · AZOR2_BACSU

Function

function

Quinone reductase that provides resistance to thiol-specific stress caused by electrophilic quinones (Probable). Contributes to resistance to 2-methylhydroquinone (2-MHQ) and catechol (PubMed:17725564, PubMed:18208493).
Exhibits NADH-dependent 2,6-dichloroindophenol (DCIP) oxidoreductase activity (PubMed:17284825).
Also exhibits azoreductase activity. Catalyzes the reductive cleavage of the azo bond in aromatic azo compounds to the corresponding amines (PubMed:17284825).
Can reduce methyl red (PubMed:17284825).

Catalytic activity

Cofactor

FMN (UniProtKB | Rhea| CHEBI:58210 )

Note: Binds 1 FMN per subunit.

Activity regulation

Strongly inhibited by Pb2+ and weakly inhibited by Cu2+, Hg2+ and Fe2+. Stable in presence of Ag+.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
8 μM2,6-dichloroondophenol (DCIP)7.525
190 μMNADH7.525

pH Dependence

Optimum pH is 7.5.

Temperature Dependence

Activity increases linearly up to 40 degrees Celsius. Stable up to 55 degrees Celsius after incubation for 30 minutes at pH 7.5. During incubation at 25 degrees Celsius for 16h, the enzyme is stable between pH 5.5 and 9.0, where 80% activity is observed.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site17-19FMN (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionelectron transfer activity
Molecular FunctionFMN binding
Molecular Functionoxidoreductase activity, acting on NAD(P)H as acceptor
Molecular Functionoxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
Biological Processcatabolic process
Biological Processresponse to toxic substance

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    FMN-dependent NADH:quinone oxidoreductase 2
  • EC number
  • Alternative names
    • Azo-dye reductase 2
    • FMN-dependent NADH-azo compound oxidoreductase 2
    • FMN-dependent NADH-azoreductase 2
      (EC:1.7.1.17
      ) . EC:1.7.1.17 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      azoR2
    • Synonyms
      yvaB
    • Ordered locus names
      BSU33540

Organism names

Accessions

  • Primary accession
    O32224

Proteomes

Phenotypes & Variants

Disruption phenotype

Deletion mutant is sensitive to 2-MHQ and catechol.

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00001663292-211FMN-dependent NADH:quinone oxidoreductase 2

Proteomic databases

Expression

Induction

Repressed by MhqR. Induced by thiol specific stress conditions, such as exposure to 2-methylhydroquinone (2-MHQ), catechol or diamide. Not induced by oxidative stress due to hydrogen peroxide or methylglyoxal.

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the azoreductase type 1 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    211
  • Mass (Da)
    23,272
  • Last updated
    1998-01-01 v1
  • Checksum
    DE8C5ABFDCA423C0
MAKVLYITAHPHDEATSYSMATGKAFIESYKEANPNDEVVHIDLYKENIPHIDADVFSGWGKLQSGTGFEELSESEKAKVGRLGELSDQFASADKYVFVTPLWNFSFPPVMKAYLDSVAVAGKSFKYTEQGPVGLLTDKKAIHIQARGGYYSEGPAAEMEMGHRYIGIMMNFFGVPSFDGIFVEGHNAEPDKAQQIKEDAIARAKEAGKTF

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL009126
EMBL· GenBank· DDBJ
CAB15359.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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