O32042 · DTD_BACSU
- ProteinInactive D-aminoacyl-tRNA deacylase
- Genedtd
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids132 (go to sequence)
- Protein existenceUncertain
- Annotation score3/5
Function
function
Miscellaneous
Culture in the presence of D-Leu, D-Met, D-Trp or D-Tyr leads to growth inhibition due to their incorporation into protein, as well as inhibition of biofilm formation (PubMed:24097941).
Partially contradictory results are seen in another study where growth occurs in the presence of 20 and 500 mg/ml D-Trp but not in 500 mg/ml D-Asp or D-Ser; whether these are the exact same strains is unknown (PubMed:25441601).
Strains in which this gene is restored by mutagenesis are resistant to D-Leu, D-Met, D-Trp and D-Tyr and form biofilm even in the presence of D-amino acids, while maintaining the ability to incorporate at least D-Trp into peptidoglycan (PubMed:24097941).
Partially contradictory results are seen in another study where growth occurs in the presence of 20 and 500 mg/ml D-Trp but not in 500 mg/ml D-Asp or D-Ser; whether these are the exact same strains is unknown (PubMed:25441601).
Strains in which this gene is restored by mutagenesis are resistant to D-Leu, D-Met, D-Trp and D-Tyr and form biofilm even in the presence of D-amino acids, while maintaining the ability to incorporate at least D-Trp into peptidoglycan (PubMed:24097941).
Biotechnology
The dtd gene from B.amyloliquefaciens strain A50 confers the ability to grow in the presence of 20 mg/ml D-Tyr and 500 mg/ml D-Asp to B.subtilis, showing it can be used as a selective marker in bacteria that do not have a functional copy of this gene. Spontaneous resistance to D-Tyr is very rare, on the order of 10-9 (PubMed:25441601).
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | D-tyrosyl-tRNA(Tyr) deacylase activity | |
Molecular Function | Gly-tRNA(Ala) hydrolase activity | |
Molecular Function | Ser(Gly)-tRNA(Ala) hydrolase activity | |
Molecular Function | tRNA binding | |
Biological Process | D-amino acid catabolic process | |
Biological Process | tRNA metabolic process |
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameInactive D-aminoacyl-tRNA deacylase
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus
Accessions
- Primary accessionO32042
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000164625 | 1-132 | Inactive D-aminoacyl-tRNA deacylase | |||
Sequence: MDEEVVGQIGQGLMVLVGITHDDTEDDAAYLADKVVNLRIFDDSEGKMNLSLVDIGGEILSVSQFTLYGDTKKGRRPNYMNAAKPDKALGLYEKWNDLLREKGIKVETGTFGAMMDVQLTNSGPVTLIMDSK |
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length132
- Mass (Da)14,487
- Last updated2017-09-27 v3
- Checksum61DFB081591E6D7B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL009126 EMBL· GenBank· DDBJ | CAB14718.2 EMBL· GenBank· DDBJ | Genomic DNA |