O32042 · DTD_BACSU

Function

function

A non-functional D-aminoacyl-tRNA deacylase (PubMed:24097941, PubMed:25441601, PubMed:4292198).

Miscellaneous

Culture in the presence of D-Leu, D-Met, D-Trp or D-Tyr leads to growth inhibition due to their incorporation into protein, as well as inhibition of biofilm formation (PubMed:24097941).
Partially contradictory results are seen in another study where growth occurs in the presence of 20 and 500 mg/ml D-Trp but not in 500 mg/ml D-Asp or D-Ser; whether these are the exact same strains is unknown (PubMed:25441601).
Strains in which this gene is restored by mutagenesis are resistant to D-Leu, D-Met, D-Trp and D-Tyr and form biofilm even in the presence of D-amino acids, while maintaining the ability to incorporate at least D-Trp into peptidoglycan (PubMed:24097941).

Caution

Could be the product of a pseudogene. The lack of an initiation codon prevents translation of this gene, in agreement with the observation that D-Tyr-tRNA(Tyr) deacylase activity cannot be detected in some strains of B.subtilis (PubMed:24097941, PubMed:25441601, PubMed:4292198).
Mutagenesis to restore the start codon (in strain NCIB 3610, the parent of the fully sequenced 168) confers resistance to D-Tyr (PubMed:24097941).

Biotechnology

The dtd gene from B.amyloliquefaciens strain A50 confers the ability to grow in the presence of 20 mg/ml D-Tyr and 500 mg/ml D-Asp to B.subtilis, showing it can be used as a selective marker in bacteria that do not have a functional copy of this gene. Spontaneous resistance to D-Tyr is very rare, on the order of 10-9 (PubMed:25441601).

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionD-tyrosyl-tRNA(Tyr) deacylase activity
Molecular FunctionGly-tRNA(Ala) hydrolase activity
Molecular FunctionSer(Gly)-tRNA(Ala) hydrolase activity
Molecular FunctiontRNA binding
Biological ProcessD-amino acid catabolic process
Biological ProcesstRNA metabolic process

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Inactive D-aminoacyl-tRNA deacylase

Gene names

    • Name
      dtd
    • Synonyms
      yrvI
    • Ordered locus names
      BSU27590

Organism names

Accessions

  • Primary accession
    O32042

Proteomes

Subcellular Location

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001646251-132Inactive D-aminoacyl-tRNA deacylase

Proteomic databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the DTD family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    132
  • Mass (Da)
    14,487
  • Last updated
    2017-09-27 v3
  • Checksum
    61DFB081591E6D7B
MDEEVVGQIGQGLMVLVGITHDDTEDDAAYLADKVVNLRIFDDSEGKMNLSLVDIGGEILSVSQFTLYGDTKKGRRPNYMNAAKPDKALGLYEKWNDLLREKGIKVETGTFGAMMDVQLTNSGPVTLIMDSK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL009126
EMBL· GenBank· DDBJ
CAB14718.2
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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