O31718 · RPOY_BACSU
- ProteinDNA-directed RNA polymerase subunit epsilon
- GenerpoY
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
A non-essential component of RNA polymerase (RNAP) (PubMed:18289874, PubMed:21710567, PubMed:25092033, PubMed:6802805).
Has a similar structure to bacteriophage T7 protein Gp2 (AC P03704), which is known to bind to RNAP in the DNA binding-cleft. Unlike Gp2 however, this protein does not inhibit transcription initiation (PubMed:25092033).
In vitro reconstitution experiments show this subunit is dispensible (PubMed:18289874).
Has a similar structure to bacteriophage T7 protein Gp2 (AC P03704), which is known to bind to RNAP in the DNA binding-cleft. Unlike Gp2 however, this protein does not inhibit transcription initiation (PubMed:25092033).
In vitro reconstitution experiments show this subunit is dispensible (PubMed:18289874).
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | DNA-directed RNA polymerase complex | |
Cellular Component | nucleoid | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit epsilon
- EC number
- Short namesRNAP epsilon subunit
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus
Accessions
- Primary accessionO31718
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Colocalizes with RNAP.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
No visible phenotype; growth, bacterial fitness in competition experiments, RNAP distribution, global transcript levels and transcription initiation in vitro are all unaffected.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 34 | No change in subcellular localization. | ||||
Sequence: R → A | ||||||
Mutagenesis | 41-50 | No longer localizes to the nucleoid. | ||||
Sequence: KFNIEFITPV → AAAAAAAAAA | ||||||
Mutagenesis | 46-48 | No change in subcellular localization. | ||||
Sequence: FIT → AAA | ||||||
Mutagenesis | 61-69 | No change in subcellular localization. | ||||
Sequence: SENFKVLEL → AAAAAAAAA |
Expression
Induction
Part of the rpoY-rnjA operon, transcribed constitutively.
Interaction
Subunit
Monomer (By similarity).
RNAP is composed of a core of 2 alpha, a beta and a beta' subunit. The core is associated with a delta subunit, and at least one of epsilon or omega (PubMed:18289874, PubMed:21710567, PubMed:25092033, PubMed:6802805).
When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (PubMed:18289874).
RNAP is composed of a core of 2 alpha, a beta and a beta' subunit. The core is associated with a delta subunit, and at least one of epsilon or omega (PubMed:18289874, PubMed:21710567, PubMed:25092033, PubMed:6802805).
When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (PubMed:18289874).
Protein-protein interaction databases
Structure
Family & Domains
Sequence similarities
Belongs to the RNA polymerase subunit epsilon family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length69
- Mass (Da)8,252
- Last updated1998-01-01 v1
- Checksum4A6F31075B01943D
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL009126 EMBL· GenBank· DDBJ | CAB13327.1 EMBL· GenBank· DDBJ | Genomic DNA |