O31612 · NADK1_BACSU
- ProteinNAD kinase 1
- GeneppnKA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids266 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP. It can use ATP and other nucleoside triphosphates (GTP, UTP) as well as inorganic polyphosphate (poly(P)) as a source of phosphorus.
Catalytic activity
- ATP + NAD+ = ADP + H+ + NADP+
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Divalent metal ions. Both Ca2+ and Mn2+ ions are more effective activators than zinc, cobalt, copper and magnesium ions at low concentrations.
Activity regulation
Allosterically inhibited by NADP and activated by quinolinic acid. Strongly inhibited by HgCl2.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.73 mM | NAD | 7.8 | 25 |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
3.82 μmol/min/mg | 7.8 | 25 |
kcat is 8 sec-1 for kinase activity with NAD (at pH 7.8 and 25 degrees Celsius).
pH Dependence
Optimum pH is 9.
Temperature Dependence
Optimum temperature is 35 degrees Celsius. Half of the activity is lost after treatment at 40 degrees Celsius for 15 minutes.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 45 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 45-46 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: DG | ||||||
Binding site | 122-123 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: NE | ||||||
Binding site | 148 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 150 | ATP (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 158 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 161-166 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: TAYNKA |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | NAD binding | |
Molecular Function | NAD+ kinase activity | |
Biological Process | NAD metabolic process | |
Biological Process | NADP biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNAD kinase 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus
Accessions
- Primary accessionO31612
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000120598 | 1-266 | NAD kinase 1 | |||
Sequence: MKFAVSSKGDQVSDTLKSKIQAYLLDFDMELDENEPEIVISVGGDGTLLYAFHRYSDRLDKTAFVGVHTGHLGFYADWVPHEIEKLVLAIAKTPYHTVEYPLLEVIVTYHENEREERYLALNECTIKSIEGSLVADVEIKGQLFETFRGDGLCLSTPSGSTAYNKALGGAIIHPSIRAIQLAEMASINNRVFRTVGSPLLLPSHHDCMIKPRNEVDFQVTIDHLTLLHKDVKSIRCQVASEKVRFARFRPFPFWKRVQDSFIGKGE |
Proteomic databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length266
- Mass (Da)30,012
- Last updated1998-01-01 v1
- Checksum82FA00BC6FE53364
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL009126 EMBL· GenBank· DDBJ | CAB13018.1 EMBL· GenBank· DDBJ | Genomic DNA |