O30477 · DNAB_RHOMR
- ProteinReplicative DNA helicase DnaB
- GenednaB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids941 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
The main replicative DNA helicase, it participates in initiation and elongation during chromosome replication. Travels ahead of the DNA replisome, separating dsDNA into templates for DNA synthesis. A processive ATP-dependent 5'-3' DNA helicase it has DNA-dependent ATPase activity.
The intein is an endonuclease.
Catalytic activity
- Couples ATP hydrolysis with the unwinding of duplex DNA at the replication fork by translocating in the 5'-3' direction. This creates two antiparallel DNA single strands (ssDNA). The leading ssDNA polymer is the template for DNA polymerase III holoenzyme which synthesizes a continuous strand.
- ATP + H2O = ADP + H+ + phosphate
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | primosome complex | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA binding | |
Molecular Function | DNA helicase activity | |
Molecular Function | endonuclease activity | |
Molecular Function | isomerase activity | |
Biological Process | DNA replication, synthesis of primer | |
Biological Process | DNA unwinding involved in DNA replication | |
Biological Process | intein-mediated protein splicing | |
Biological Process | intron homing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameReplicative DNA helicase DnaB
- EC number
- Alternative names
- Cleaved into 1 chains
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Rhodothermota > Rhodothermia > Rhodothermales > Rhodothermaceae > Rhodothermus
Accessions
- Primary accessionO30477
- Secondary accessions
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000013285 | 1-421 | Replicative DNA helicase DnaB, 1st part | |||
Sequence: MAEFEERPRLSIGEEEAPPYPLEKLTGGRRTRAQIHALHQQAGRVPPQAVELEQAVLGAMLIEPEAIPRALEILTPEAFYDGRHQRIFRAIVRLFEQNRGVDLLTVTEELRRTGELEQAGDTIYLSELTTRVASAANVEYHARIIAEKLLRRMIEVMTLLVGRAYDPAADAFELLDEVEAEIFRLSDVHLRKAARSMNEVVKETLERLEAIHGRPGGITGVPSGFHQLDALTGGWQRGDLIIIAARPSMGKTAFALSCRNAALHPHYGTGVAIFSLEMGAEQLAQRLLTAEAASMPRRPAPDGCATRTGVSWPARRPLSDAPIFIDDTPSLGVLELRAKCRRLKAEHDIGLVIVDYLQLMQASHMPRNANREQEIAQISRSLKALAKELNVPVVALSQLSRAVETRGGDKRPQLSDLRESG | ||||||
Chain | PRO_0000013286 | 422-849 | Homing endonuclease PI-Rma43812IP | |||
Sequence: CLAGDTLITLADGRRVPIRELVSQQNFSVWALNPQTYRLERARVSRAFCTGIKPVYRLTTRLGRSIRATANHRFLTPQGWKRVDELQPGDYLALPRRIPTASTPTLTEAELALLGHLIGDGCTLPHHVIQYTSRDADLATLVAHLATKVFGSKVTPQIRKELRWYQVYLRAARPLAPGKRNPISDWLRDLGIFGLRSYEKKVPALLFCQTSEAIATFLRHLWATDGCIQMRRGKKPYPAVYYATSSYQLARDVQSLLLRLGINARLKTVAQGEKGRVQYHVKVSGREDLLRFVEKIGAVGARQRAALASVYDYLSVRTGNPNRDIIPVALWYELVREAMYQRGISHRQLHANLGMAYGGMTLFRQNLSRARALRLAEAAACPELRQLAQSDVYWDPIVSIEPDGVEEVFDLTVPGPHNFVANDIIAHN | ||||||
Chain | PRO_0000013287 | 850-941 | Replicative DNA helicase DnaB, 2nd part | |||
Sequence: SIEQDADVVLFIYRPERYGITVDENGNPTEGIAEIIIGKQRNGPTGTVRLAFINQYARFENLTMYQPEPGTPLPETPDETILPSGPPDEAPF |
Post-translational modification
Upon expression in E.coli this protein undergoes self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.
Keywords
- PTM
Interaction
Subunit
Homohexamer.
Structure
Family & Domains
Features
Showing features for region, domain, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-25 | Disordered | ||||
Sequence: MAEFEERPRLSIGEEEAPPYPLEKL | ||||||
Domain | 214-484 | SF4 helicase; first part | ||||
Sequence: RPGGITGVPSGFHQLDALTGGWQRGDLIIIAARPSMGKTAFALSCRNAALHPHYGTGVAIFSLEMGAEQLAQRLLTAEAASMPRRPAPDGCATRTGVSWPARRPLSDAPIFIDDTPSLGVLELRAKCRRLKAEHDIGLVIVDYLQLMQASHMPRNANREQEIAQISRSLKALAKELNVPVVALSQLSRAVETRGGDKRPQLSDLRESGCLAGDTLITLADGRRVPIRELVSQQNFSVWALNPQTYRLERARVSRAFCTGIKPVYRLTTRLG | ||||||
Domain | 534-683 | DOD-type homing endonuclease | ||||
Sequence: LLGHLIGDGCTLPHHVIQYTSRDADLATLVAHLATKVFGSKVTPQIRKELRWYQVYLRAARPLAPGKRNPISDWLRDLGIFGLRSYEKKVPALLFCQTSEAIATFLRHLWATDGCIQMRRGKKPYPAVYYATSSYQLARDVQSLLLRLGI | ||||||
Domain | 646-915 | SF4 helicase; second part | ||||
Sequence: DGCIQMRRGKKPYPAVYYATSSYQLARDVQSLLLRLGINARLKTVAQGEKGRVQYHVKVSGREDLLRFVEKIGAVGARQRAALASVYDYLSVRTGNPNRDIIPVALWYELVREAMYQRGISHRQLHANLGMAYGGMTLFRQNLSRARALRLAEAAACPELRQLAQSDVYWDPIVSIEPDGVEEVFDLTVPGPHNFVANDIIAHNSIEQDADVVLFIYRPERYGITVDENGNPTEGIAEIIIGKQRNGPTGTVRLAFINQYARFENLTMYQ | ||||||
Region | 914-941 | Disordered | ||||
Sequence: YQPEPGTPLPETPDETILPSGPPDEAPF | ||||||
Compositional bias | 919-941 | Pro residues | ||||
Sequence: GTPLPETPDETILPSGPPDEAPF |
Sequence similarities
Belongs to the helicase family. DnaB subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length941
- Mass (Da)104,883
- Last updated1998-01-01 v1
- Checksum9145C41066152B29
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 30 | in Ref. 2; CAA74140 | ||||
Sequence: R → RR | ||||||
Sequence conflict | 148 | in Ref. 2; CAA74140 | ||||
Sequence: K → KA | ||||||
Sequence conflict | 258 | in Ref. 2; CAA74140 | ||||
Sequence: C → CA | ||||||
Sequence conflict | 293-317 | in Ref. 2; CAA74140 | ||||
Sequence: ASMPRRPAPDGCATRTGVSWPARRP → RVDAQAARTGRLRDEDWRKLARAAGR | ||||||
Compositional bias | 919-941 | Pro residues | ||||
Sequence: GTPLPETPDETILPSGPPDEAPF |