O30394 · TYSY_BACAT

  • Protein
    Thymidylate synthase
  • Gene
    thyA
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.

Catalytic activity

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Features

Showing features for binding site, active site.

122720406080100120140160180200220
TypeIDPosition(s)Description
Binding site89-90dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Active site109Nucleophile
Binding site129-132dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site132(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site140dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site170-172dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionthymidylate synthase activity
Biological ProcessdTMP biosynthetic process
Biological ProcessdTTP biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Thymidylate synthase
  • EC number
  • Short names
    TS
    ; TSase
  • Alternative names
    • Thymidylate synthase A
      (TS A
      ; TSase A
      )

Gene names

    • Name
      thyA

Organism names

  • Taxonomic identifier
  • Strain
    • S223 / B0099 / 1208
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus

Accessions

  • Primary accession
    O30394

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001409191-227Thymidylate synthase

Interaction

Subunit

Homodimer.

Structure

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    227
  • Mass (Da)
    26,901
  • Last updated
    1998-01-01 v1
  • Checksum
    15B33930C329FAAB
SEVPILTTKKLAWKTAIKELLWIWQLKSNDVNDLNKMSVHIWDQWKQEDGTIGHAYGFQLGKKNRSLNGEKVDQVDYLLHQLKNNPSSRRHITMLWNPDELDAMALTPCVYETQWYVKHGKLHLEVRARSNDMALGNPFNVFQYNVLQRMIAQVTGYELGEYIFNIGDCHVYTRHIDNLKIQMEREQFEAPELWINPEVKDFYDFTIDDFKLINYKHGDKLLFEVRV

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue1

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF004101
EMBL· GenBank· DDBJ
AAC26323.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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