O29370 · FNO_ARCFU
- ProteinF420-dependent NADP reductase
- Genefno
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids212 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the reversible reduction of NADP+ by F420H2. In this reaction the proS hydrogen at C5 of F420 is transferred into the proS position at C4 of NADPH.
Catalytic activity
- NADP+ + reduced coenzyme F420-(gamma-L-Glu)(n) = 2 H+ + NADPH + oxidized coenzyme F420-(gamma-L-Glu)(n)
CHEBI:58349 + reduced coenzyme F420-(γ-L-Glu)(n) RHEA-COMP:14378 = 2 CHEBI:15378 + CHEBI:57783 + oxidized coenzyme F420-(γ-L-Glu)(n) RHEA-COMP:12939
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
20 μM | reduced coenzyme F420 | 8.0 | 65 | |||
40 μM | NADP+ | 8.0 | 65 | |||
10 μM | oxidized coenzyme F420 | 5.5 | 65 | |||
40 μM | NADPH | 5.5 | 65 |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
660 μmol/min/mg | 5.5 | 80 | for NADP reduction with F420H2 |
pH Dependence
Optimum pH is 8.0 for NADP reduction with F420H2. Optimum pH is 5.5 for F420 reduction with NADPH.
Temperature Dependence
Optimum temperature is 80 degrees Celsius. Is highly thermostable.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 9-12 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: TGNL | ||||||
Binding site | 31-32 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: SR | ||||||
Binding site | 36 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 72 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 76 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 98 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: V | ||||||
Binding site | 137 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 207 | coenzyme F420-(gamma-Glu)n (UniProtKB | ChEBI) | ||||
Sequence: L |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | 8-hydroxy-5-deazaflavin:NADPH oxidoreductase activity | |
Molecular Function | coenzyme F420 binding | |
Molecular Function | cupric reductase activity | |
Molecular Function | ferric-chelate reductase (NADPH) activity | |
Molecular Function | NADP binding | |
Molecular Function | reduced coenzyme F420:NADP+ oxidoreductase activity | |
Biological Process | copper ion import | |
Biological Process | NADPH regeneration |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameF420-dependent NADP reductase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Archaeoglobi > Archaeoglobales > Archaeoglobaceae > Archaeoglobus
Accessions
- Primary accessionO29370
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000419113 | 1-212 | F420-dependent NADP reductase | |||
Sequence: MRVALLGGTGNLGKGLALRLATLGHEIVVGSRREEKAEAKAAEYRRIAGDASITGMKNEDAAEACDIAVLTIPWEHAIDTARDLKNILREKIVVSPLVPVSRGAKGFTYSSERSAAEIVAEVLESEKVVSALHTIPAARFANLDEKFDWDVPVCGDDDESKKVVMSLISEIDGLRPLDAGPLSNSRLVESLTPLILNIMRFNGMGELGIKFL |
Proteomic databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length212
- Mass (Da)22,865
- Last updated1998-01-01 v1
- Checksum16E2F079C9F471CE
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE000782 EMBL· GenBank· DDBJ | AAB90348.1 EMBL· GenBank· DDBJ | Genomic DNA |