O27336 · HJC_METTH
- ProteinCrossover junction endodeoxyribonuclease Hjc
- Genehjc
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids136 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are subsequently ligated to produce recombinant products. Cleaves both mobile and immobile junctions. Converts a synthetic Hj to nicked duplex products. Binds a variety of branched DNA structures but linear dsDNA poorly; cleaves only 4-way junctions. Confers UV and mitomycin C resistance to E.coli cells lacking the endogenous Hj machinery RuvABC.
Catalytic activity
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
pH Dependence
Optimum pH is 7.8-9.5.
Temperature Dependence
Optimum temperature is 55 degrees Celsius.
Features
Showing features for binding site, active site, site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | crossover junction DNA endonuclease activity | |
Molecular Function | DNA binding | |
Molecular Function | magnesium ion binding | |
Biological Process | DNA recombination | |
Biological Process | DNA repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCrossover junction endodeoxyribonuclease Hjc
- EC number
- Short namesHjc
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Methanomada group > Methanobacteria > Methanobacteriales > Methanobacteriaceae > Methanothermobacter
Accessions
- Primary accessionO27336
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000429155 | 1-136 | Crossover junction endodeoxyribonuclease Hjc | |||
Sequence: MVKNGTRGERDLVKLLWEKGFAAMRAPASGGATKKPLPDIIAGNGEIYLAIEVKTTARERIYIDSEKIGALLRFSDIFGARPYIGIKFRYRDWIFLSPGDLELTPSSNYRLDLDIALERGRDLDEVTGNHRQTRLR |
Proteomic databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length136
- Mass (Da)15,396
- Last updated1998-01-01 v1
- ChecksumFE5D18497D97CFC3
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE000666 EMBL· GenBank· DDBJ | AAB85757.1 EMBL· GenBank· DDBJ | Genomic DNA |