O27118 · O27118_METTH
- ProteinS-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
- Genetaw1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids305 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Component of the wyosine derivatives biosynthesis pathway that catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine (imG-14) on guanosine-37 of tRNA(Phe).
Catalytic activity
- N1-methylguanosine37 in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine37 in tRNA(Phe) + 5'-deoxyadenosine + CO2 + H2O + L-methionine
RHEA-COMP:10165 CHEBI:73542 Position: 37+ CHEBI:15361 + CHEBI:59789 = RHEA-COMP:10164 CHEBI:64315 Position: 37+ CHEBI:17319 + CHEBI:16526 + CHEBI:15377 + CHEBI:57844
Cofactor
Note: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 31 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 44 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 57 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 67 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 71 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 74 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | tRNA-4-demethylwyosine synthase activity | |
Biological Process | tRNA processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameS-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Methanomada group > Methanobacteria > Methanobacteriales > Methanobacteriaceae > Methanothermobacter
Accessions
- Primary accessionO27118
Proteomes
Subcellular Location
PTM/Processing
Proteomic databases
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 50-291 | Radical SAM core | ||||
Sequence: YGIRSHRCLQMSPSVPFCQQKCLFCWRDLSSTGTAWEGPHDEPADIIDGAIEAQRKLLCGYLGNERADKIKVMESQDPTNAAISLAGEPMLYPDMDGLLREFHRRNFTTFLVTNGLAPMNLEKLSEEPTQLYISLDAPDRDTYKELCRPQIPGAWDLLNSSLELMPSFSCRKVLRITAVRHINMKDPEGFARMIERARPDFVEVKAYMYIGYSRRRLDIENMPLFYEVHEFAEELAAASGME |
Sequence similarities
Belongs to the TYW1 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length305
- Mass (Da)35,059
- Last updated1998-01-01 v1
- ChecksumE90091C2F09775DA
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE000666 EMBL· GenBank· DDBJ | AAB85535.1 EMBL· GenBank· DDBJ | Genomic DNA |