O27118 · O27118_METTH

Function

function

Component of the wyosine derivatives biosynthesis pathway that catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine (imG-14) on guanosine-37 of tRNA(Phe).

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site31[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site44[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site57[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site67[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site71[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site74[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular FunctiontRNA-4-demethylwyosine synthase activity
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
  • EC number
  • Alternative names
    • tRNA wyosine derivatives biosynthesis protein Taw1

Gene names

    • Name
      taw1
    • Ordered locus names
      MTH_1039

Organism names

Accessions

  • Primary accession
    O27118

Proteomes

Subcellular Location

Keywords

PTM/Processing

Proteomic databases

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain50-291Radical SAM core

Sequence similarities

Belongs to the TYW1 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    305
  • Mass (Da)
    35,059
  • Last updated
    1998-01-01 v1
  • Checksum
    E90091C2F09775DA
MAMYLEESQRKMERMGYRFVGEHGHSAVKTCLWTKKSIVNEGVCYKEKFYGIRSHRCLQMSPSVPFCQQKCLFCWRDLSSTGTAWEGPHDEPADIIDGAIEAQRKLLCGYLGNERADKIKVMESQDPTNAAISLAGEPMLYPDMDGLLREFHRRNFTTFLVTNGLAPMNLEKLSEEPTQLYISLDAPDRDTYKELCRPQIPGAWDLLNSSLELMPSFSCRKVLRITAVRHINMKDPEGFARMIERARPDFVEVKAYMYIGYSRRRLDIENMPLFYEVHEFAEELAAASGMEIVDESRESRVVLLA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE000666
EMBL· GenBank· DDBJ
AAB85535.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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