O25511 · PSEB_HELPY

Function

function

Catalyzes the first step in the biosynthesis of pseudaminic acid, a sialic-acid-like sugar that is used to modify flagellin. Has both C6 dehydratase and C5 epimerase activities that result in the production of both UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose and UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose.

Catalytic activity

Cofactor

NADP+ (UniProtKB | Rhea| CHEBI:58349 )

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
159 μMUDP-GlcNAc

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site19-22NADP+ (UniProtKB | ChEBI)
Binding site43-48NADP+ (UniProtKB | ChEBI)
Binding site67-68NADP+ (UniProtKB | ChEBI)
Binding site87NADP+ (UniProtKB | ChEBI)
Binding site91NADP+ (UniProtKB | ChEBI)
Binding site91substrate
Binding site129-130NADP+ (UniProtKB | ChEBI)
Active site133
Binding site141NADP+ (UniProtKB | ChEBI)
Binding site145NADP+ (UniProtKB | ChEBI)
Binding site173substrate
Binding site174-178NADP+ (UniProtKB | ChEBI)
Binding site181substrate
Binding site199substrate
Binding site258substrate
Binding site261substrate

GO annotations

AspectTerm
Molecular Functionlyase activity
Molecular Functionnucleotide binding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    UDP-N-acetylglucosamine 4,6-dehydratase (inverting)
  • EC number
  • Alternative names
    • Pseudaminic acid biosynthesis protein B
    • UDP-GlcNAc-inverting 4,6-dehydratase

Gene names

    • Name
      pseB
    • Synonyms
      flaA1
    • Ordered locus names
      HP_0840

Organism names

Accessions

  • Primary accession
    O25511

Proteomes

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis133Loss of activity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004189541-333UDP-N-acetylglucosamine 4,6-dehydratase (inverting)

Proteomic databases

Interaction

Subunit

Homohexamer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the polysaccharide synthase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    333
  • Mass (Da)
    37,354
  • Last updated
    1998-01-01 v1
  • Checksum
    8EF0A39F0FBF0516
MPNHQNMLDNQTILITGGTGSFGKCFVRKVLDTTNAKKIIVYSRDELKQSEMAMEFNDPRMRFFIGDVRDLERLNYALEGVDICIHAAALKHVPIAEYNPLECIKTNIMGASNVINACLKNAISQVIALSTDKAANPINLYGATKLCSDKLFVSANNFKGSSQTQFSVVRYGNVVGSRGSVVPFFKKLVQNKASEIPITDIRMTRFWITLDEGVSFVLKSLKRMHGGEIFVPKIPSMKMTDLAKALAPNTPTKIIGIRPGEKLHEVMIPKDESHLALEFEDFFIIQPTISFQTPKDYTLTKLHEKGQKVAPDFEYSSHNNNQWLEPDDLLKLL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE000511
EMBL· GenBank· DDBJ
AAD07887.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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